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Gene Review

Znhit2  -  zinc finger, HIT domain containing 2

Mus musculus

Synonyms: C11orf5, Fon, ORF6, Protein FON, Zinc finger HIT domain-containing protein 2, ...
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Disease relevance of Znhit2

  • A prime candidate is ORF6, which encodes a 63-amino-acid membrane-associated peptide that can dramatically increase the lethality of an otherwise attenuated JHM strain of murine coronavirus (L. Pewe, H. Zhou, J. Netland, C. Tangudu, H. Olivares, L. Shi, D. Look, T. Gallagher, and S. Perlman, J. Virol. 79:11335-11342, 2005) [1].
  • To determine the significance of the SARS-CoV group-specific ORFs in virus replication in vitro and in mice, we systematically deleted five of the eight group-specific ORFs, ORF3a, OF3b, ORF6, ORF7a, and ORF7b, and characterized recombinant virus replication and gene expression in vitro [2].
  • DNA and RNA analysis experiments verified that the mouse mammary tumor virus promoter drives the expression of the ORF6+ORF7 unit and permits its bona fide alternative splicing, generating ORF6/7 mRNA in addition to the ORF6-expressing primary transcript [3].
  • ORF 7 encodes the virion nucleocapsid protein Vp-1, while ORF 6 encodes the nonglycosylated envelope protein Vp2 [4].
  • The ability of these heterodimers to serve as transcriptional regulators is modulated by complex formation with additional proteins such as the products of the retinoblastoma gene and the adenovirus E4 ORF 6/7 [5].

High impact information on Znhit2

  • Antisera directed against ORF4-related sequences did not recognize M. pneumoniae antigens in Western blot analysis, but antisera directed against ORF-6-derived fusion proteins reacted with two M. pneumoniae proteins of 40 kDa and 90 kDa [6].
  • One protein, the product of SARS-CoV ORF6, converted a sublethal infection to a uniformly lethal encephalitis and enhanced virus growth in tissue culture cells, indicating that SARS-CoV proteins function in the context of a heterologous coronavirus infection [7].
  • ORF5 and ORF6 (which encodes the M protein) of EAV were cloned into two different VEE replicon vectors that contained either one or two 26S subgenomic mRNA promoters [8].
  • The mgc3 gene is a homologue of the ORF6 gene encoding 130-kDa protein in the P1 operon of M. pneumoniae and is localized downstream of the mgc1 gene, a homologue of the P1 gene [9].
  • Mutational analysis showed that the marked box contributed to the recognition of E2F family members by the E4 ORF 6/7 protein in vitro and in vivo [5].

Chemical compound and disease context of Znhit2


Biological context of Znhit2

  • Molecular characterization of human and murine C11orf5, a new member of the FAUNA gene cluster [11].

Anatomical context of Znhit2

  • The highest lymphocyte proliferation responses were also observed in mice immunized with pCI-ORF5/ORF6 [12].
  • Analysis of protein expression by mammalian cell lines stably expressing lactate dehydrogenase-elevating virus ORF 5 and ORF 6 proteins [13].

Analytical, diagnostic and therapeutic context of Znhit2

  • Interestingly, significantly enhanced GP5-specific ELISA antibody could be detected in mice immunized with pCI-ORF5/ORF6 compared to mice immunized with pCI-ORF5 [12].


  1. Severe acute respiratory syndrome coronavirus protein 6 accelerates murine coronavirus infections. Tangudu, C., Olivares, H., Netland, J., Perlman, S., Gallagher, T. J. Virol. (2007) [Pubmed]
  2. Severe acute respiratory syndrome coronavirus group-specific open reading frames encode nonessential functions for replication in cell cultures and mice. Yount, B., Roberts, R.S., Sims, A.C., Deming, D., Frieman, M.B., Sparks, J., Denison, M.R., Davis, N., Baric, R.S. J. Virol. (2005) [Pubmed]
  3. Efficient dual transcomplementation of adenovirus E1 and E4 regions from a 293-derived cell line expressing a minimal E4 functional unit. Yeh, P., Dedieu, J.F., Orsini, C., Vigne, E., Denefle, P., Perricaudet, M. J. Virol. (1996) [Pubmed]
  4. Complete genomic sequence and phylogenetic analysis of the lactate dehydrogenase-elevating virus (LDV). Godeny, E.K., Chen, L., Kumar, S.N., Methven, S.L., Koonin, E.V., Brinton, M.A. Virology (1993) [Pubmed]
  5. A conserved region of unknown function participates in the recognition of E2F family members by the adenovirus E4 ORF 6/7 protein. Jost, C.A., Ginsberg, D., Kaelin, W.G. Virology (1996) [Pubmed]
  6. Identification of gene products of the P1 operon of Mycoplasma pneumoniae. Sperker, B., Hu, P., Herrmann, R. Mol. Microbiol. (1991) [Pubmed]
  7. A severe acute respiratory syndrome-associated coronavirus-specific protein enhances virulence of an attenuated murine coronavirus. Pewe, L., Zhou, H., Netland, J., Tangudu, C., Olivares, H., Shi, L., Look, D., Gallagher, T., Perlman, S. J. Virol. (2005) [Pubmed]
  8. Expression of the two major envelope proteins of equine arteritis virus as a heterodimer is necessary for induction of neutralizing antibodies in mice immunized with recombinant Venezuelan equine encephalitis virus replicon particles. Balasuriya, U.B., Heidner, H.W., Hedges, J.F., Williams, J.C., Davis, N.L., Johnston, R.E., MacLachlan, N.J. J. Virol. (2000) [Pubmed]
  9. Identification and expression of a Mycoplasma gallisepticum surface antigen recognized by a monoclonal antibody capable of inhibiting both growth and metabolism. Yoshida, S., Fujisawa, A., Tsuzaki, Y., Saitoh, S. Infect. Immun. (2000) [Pubmed]
  10. Characterization of lactate dehydrogenase-elevating virus ORF6 protein expressed by recombinant baculoviruses. Takahashi-Omoe, H., Omoe, K., Matsushita, S., Inada, T. Comp. Immunol. Microbiol. Infect. Dis. (2004) [Pubmed]
  11. Molecular characterization of human and murine C11orf5, a new member of the FAUNA gene cluster. Lemmens, I.H., Farnebo, F., Piehl, F., Merregaert, J., Van de Ven, W.J., Larsson, C., Kas, K. Mamm. Genome (2000) [Pubmed]
  12. DNA vaccines co-expressing GP5 and M proteins of porcine reproductive and respiratory syndrome virus (PRRSV) display enhanced immunogenicity. Jiang, Y., Xiao, S., Fang, L., Yu, X., Song, Y., Niu, C., Chen, H. Vaccine (2006) [Pubmed]
  13. Analysis of protein expression by mammalian cell lines stably expressing lactate dehydrogenase-elevating virus ORF 5 and ORF 6 proteins. Takahashi-Omoe, H., Omoe, K., Sakaguchi, M., Kameoka, Y., Matsushita, S., Inada, T. Comp. Immunol. Microbiol. Infect. Dis. (2004) [Pubmed]
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