Disease relevance of CTA_0078

• Among 435 boys who agreed to undergo urethral culture, the esterase was positive in 66 (15%), Chlamydia trachomatis was isolated from 39 (9%), and Neisseria gonorrhoeae was isolated from 14 (3%) [1].
• Using the esterase test to screen asymptomatic males for urethritis, we identified 38 culture-verified infections that otherwise would have remained undetected [1].
• Dipstick leukocyte esterase activity in first-catch urine specimens. A useful screening test for detecting sexually transmitted disease in the adolescent male [2].
• Urinalysis is also of great value in symptomatic patients; the presence of pyuria (and possibly indirect quantitation of pyuria by the leukocyte esterase test) is a reliable indicator of treatable infection, and its absence indicates infection is not present [3].
• RESULTS: Of 4757 arrestees screened, 82 (1.7%) had untreated syphilis, and, of 4174 male arrestees screened, 541 (13%) had a positive leukocyte esterase test [4].

High impact information on CTA_0078

• Urinary leukocyte esterase screening test for asymptomatic chlamydial and gonococcal infections in males [1].
• This should be analysed by dipsticks incorporating nitrite and leucocyte esterase tests [5].
• When compare to positive culture results for either Chlamydia or gonorrhea, urinary leukocyte esterase activity had a sensitivity of 78%, specificity of 91%, and positive predictive value of 44% [6].
• The usefulness of screening for chlamydial trachomatis infection with cervical mucus leukocyte esterase [7].
• Leukocyte esterase dipstick tests lack both sensitivity and specificity for the detection of inflammatory changes in semen [8].

Chemical compound and disease context of CTA_0078

• Urethritis associated with Chlamydia trachomatis: comparison of leukocyte esterase dipstick test of first-voided urine and methylene blue-stained urethral smear as predictors of chlamydial infection [9].
• DESIGN--EIA for detection of Chlamydia trachomatis antigen and confirmation with immunofluorescence test (DFA) in urine, cervical and urethral chlamydia culture, nitrite and granulocyte esterase test, urine sediment, chamber count, dipslide and conventional urine culture were performed [10].

Biological context of CTA_0078

• RESULTS: The numbers of men positive by testing of FVU with nucleic acid amplification (LCx chlamydia), nucleic acid hybridization (PACE 2), enzyme immunoassay (Chlamydiazyme), and a leukocyte esterase dipstick were 26, 7, 14, and 11, respectively; urethral culture identified 6 of the infected men [11].

Analytical, diagnostic and therapeutic context of CTA_0078

• OBJECTIVE: To define the prevalence of C. trachomatis among teenagers by using the ligase chain reaction assay on urine specimens and to evaluate leukocyte esterase testing of urine specimens as an indicator of infection [12].
• We collected first-voided urine specimens from 659 males attending a sexually transmitted disease clinic and performed both enzyme immunoassay (EIA) for detection of chlamydial antigen and leukocyte esterase testing on these urine samples [13].
• Predicting Neisseria gonorrhoeae and Chlamydia trachomatis infection using risk scores, physical examination, microscopy, and leukocyte esterase urine dipsticks among asymptomatic women attending a family planning clinic in Kenya [14].
• Compared with direct immunofluorescence test of first-void urine, the urine leukocyte esterase test had a sensitivity of 93.6% and a specificity of 94.3% [15].
• Non-invasive sampling for detection of genital infection with Chlamydia trachomatis in males utilising urinary leukocyte esterase tests and immunoassays [15].

References