The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

SLC9A3  -  solute carrier family 9, subfamily A (NHE3...

Sus scrofa

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of NHE3

  • These findings suggest that the use of NHE3 blockers may decrease the duration of apnea and possibly reduce the pathophysiologic consequences of potentially life-threatening apnea in infants [1].
  • Hence, the use of NHE3 inhibitors may reduce reflexly mediated respiratory depression and duration of apnea in the neonatal period [1].
  • We tested the hypothesis that pharmacological inhibition of NHE-3 reduces albumin-induced NF-kappaB activation - and therefore albumin-induced renal interstitial inflammation and fibrosis - using established proximal tubular cell lines (OK and LLC-PK1) [2].
 

High impact information on NHE3

 

Biological context of NHE3

  • In the present study, a combination of reverse transcription-PCR, 5' RACE, and genomic library screening was used to clone the coding region of the porcine NHE3 gene [4].
  • The exchanger activity at 48 hr after transfection was mostly due to NHE-3 (as shown by inhibition in the presence of PMA) but was significantly lower than in sham transfected cells (1.2 nmoles/mg prot. in NHE-2-transfected and 2.1 in sham-transfected, P < 0.05, n = 4) [5].
  • We conclude that glycosylation of the Na+/H+ exchanger isoform NHE-3 is essential for antiporter activity in LLC-PK, cells [6].
 

Anatomical context of NHE3

 

Associations of NHE3 with chemical compounds

  • NHE-3 immunoblots of whole cell extract from tunicamycin-treated cells showed that in addition to the 95 kd protein, an 87 kd band was also detected [6].
  • Recent studies have shown that NHE-3 is the luminal Na+/H+ exchanger isoform in cultured renal proximal tubule cells LLC-PK1 and OK (J Biol Chem 1994; 269:15613-8) [6].
 

Regulatory relationships of NHE3

  • NHE-2 is predominantly expressed in the inner medulla whereas NHE-3 is highly expressed in the proximal tubule cells [5].
 

Other interactions of NHE3

  • Transient expression of Na+/H+ exchanger isoform NHE-2 in LLC-PK1 cells: inhibition of endogenous NHE-3 and regulation by hypertonicity [5].
 

Analytical, diagnostic and therapeutic context of NHE3

  • We have studied two mammalian isoforms, NHE1 (ubiquitous) and NHE3 (epithelial-specific), by measuring extracellular proton (H+) gradients during whole-cell patch clamp with perfusion of the cell interior [3].
  • In situ hybridization with two different NHE3 probes gave intense labeling of the distal convoluted tubule in pig kidney but (unexpectedly) no detectable labeling of the proximal tubule [4].

References

  1. Inhibition of Na(+)/H(+) exchanger type 3 reduces duration of apnea induced by laryngeal stimulation in piglets. Abu-Shaweesh, J.M., Dreshaj, I.A., Martin, R.J., Wirth, K.J., Heinelt, U., Haxhiu, M.A. Pediatr. Res. (2002) [Pubmed]
  2. Inhibition of Na superset+/H superset+ exchange decreases albumin-induced NF-kappaB activation in renal proximal tubular cell lines (OK and LLC-PK1 cells). Drumm, K., Gassner, B., Silbernagl, S., Gekle, M. Eur. J. Med. Res. (2001) [Pubmed]
  3. Lipid- and mechanosensitivities of sodium/hydrogen exchangers analyzed by electrical methods. Fuster, D., Moe, O.W., Hilgemann, D.W. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  4. Molecular cloning of NHE3 from LLC-PK1 cells and localization in pig kidney. Shugrue, C.A., Obermüller, N., Bachmann, S., Slayman, C.W., Reilly, R.F. J. Am. Soc. Nephrol. (1999) [Pubmed]
  5. Transient expression of Na+/H+ exchanger isoform NHE-2 in LLC-PK1 cells: inhibition of endogenous NHE-3 and regulation by hypertonicity. Singh, G., Orlowski, J., Soleimani, M. J. Membr. Biol. (1996) [Pubmed]
  6. Inhibition of glycosylation decreases Na+/H+ exchange activity, blocks NHE-3 transport to the membrane, and increases NHE-3 mRNA expression in LLC-PK1 cells. Soleimani, M., Singh, G., Bookstein, C., Rao, M.C., Chang, E.B., Dominguez, J.H. J. Lab. Clin. Med. (1996) [Pubmed]
  7. NHE3: a Na+/H+ exchanger isoform of renal brush border. Biemesderfer, D., Pizzonia, J., Abu-Alfa, A., Exner, M., Reilly, R., Igarashi, P., Aronson, P.S. Am. J. Physiol. (1993) [Pubmed]
  8. Effect of long-term hyperosmolality on the Na+/H+ exchanger isoform NHE-3 in LLC-PK1 cells. Soleimani, M., Watts, B.A., Singh, G., Good, D.W. Kidney Int. (1998) [Pubmed]
 
WikiGenes - Universities