Disease relevance of nusG

• Escherichia coli nusG and rho mutants, which are defective in transcription termination, are killed following transformation with several ColE1-like plasmids that lack the plasmid-encoded copy-number regulator gene rom because of uncontrolled plasmid replication within the cells [1].
• The nusG gene of Streptomyces griseus: cloning of the gene and analysis of the A-factor binding properties of the gene product [2].
• The nusG gene of Thermus thermophilus HB8 was cloned and sequenced [3].
• As these mutations block the transcription antitermination activity of bacteriophage lambda N gene product, they paradoxically seem to enhance transcription termination at phage termination sites [4].
• Crystal structure of AmiC: the controller of transcription antitermination in the amidase operon of Pseudomonas aeruginosa [5].

High impact information on nusG

• A bacterial gene involved in transcription antitermination: regulation at a rho-independent terminator in the bgl operon of E. coli [6].
• Transcription antitermination by phage lambda gene Q protein requires a DNA segment spanning the RNA start site [7].
• In addition, transcription antitermination activity was demonstrated for WCSP1 by using an E. coli strain that has a hairpin loop upstream of a chloramphenicol resistance gene [8].
• The N protein from bacteriophage lambda is a key regulator of transcription antitermination [9].
• tRNA-mediated transcription antitermination in vitro: codon-anticodon pairing independent of the ribosome [10].

Chemical compound and disease context of nusG

• Expression of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and tryptophan-induced transcription antitermination [11].
• The metIC operon involved in methionine biosynthesis in Bacillus subtilis is controlled by transcription antitermination [12].

Biological context of nusG

• Rif(r) mutations that changed residue Q469 to a basic residue (Q469K and Q469R) enhanced autoregulation of nusG [13].
• The nusG gene of Streptomyces griseus was cloned and the nucleotide sequence determined [2].
• The nucleic acid melting activity of Escherichia coli CspE is critical for transcription antitermination and cold acclimation of cells [14].
• Regulation of the Escherichia coli K5 capsule gene cluster by transcription antitermination [15].
• Gene expression in lambdoid phages in part is controlled by transcription antitermination [16].

Associations of nusG with chemical compounds

• The nusG gene of S. griseus does not seem to encode the A-factor-binding protein [2].
• We conclude that the tna operon of P. vulgaris is also regulated by tryptophan-induced transcription antitermination [17].

Physical interactions of nusG

• This site acts in cis in the form of RNA to assemble a transcription antitermination complex which is composed of lambda N protein and at least four host factors [18].

Other interactions of nusG

• To locate the site required for transcription antitermination by the gene Q product, we constructed a plasmid containing the p'R promoter, the t'R1 terminator, and gene galK [19].

Analytical, diagnostic and therapeutic context of nusG

• We propose that an increased occurrence of chromosomal R-loops in the rho and nusG mutants leads to titration of a cyloplasmic host factor(s) that negatively modulates the stability of plasmid R-loop replication intermediates and consequently to runaway plasmid replication [20].

References