High impact information on poly

• The metabolism of poly (A)+ and poly(A)-hnRNA in cultured Drosophila cells studied with a rapid uridine pulse-chase [1].
• A DNA polymorphism with 62% heterozygosity due to variation of a poly (T) region in the 3' UTR of the hW has been identified and used for the incorporation of this gene to the genetic map of chromosome 21 [2].
• The antibodies that reacted only with poly(dG-dC) and poly (dG-dC)n plasmid inserts did not stain chromosomes fixed by either protocol [3].
• Using in vitro RNA binding assays, we found that Rbp9 binds strongly to poly U sequences [4].
• Transcription was directed by an unusual initiator element that contained a 13 bp poly d(A) tract flanked by palindromic and inverted repeat sequences [5].

Biological context of poly

• One class, with broad specificity, reacted well with all sequences in the Z-form, including poly(dG-dC), poly(dG-dm5C), and poly (dG-dBr5C) in linear polymers and poly(dG-dC)n and poly[(dC-dA)n.(dT-dG)n] sequences in supercoiled plasmids [3].
• The two mosquito elements each have poly A tails and a polyadenylation signal, but the extreme 3' and 5' ends show no other similarity to each other or to any other insertion element [6].
• The lambdagt clones containing fragments of the Drosophila melanogaster genome were prepared and characterized by hybridization of their DNA with (I) lambdagt-cRNA; (2) lambdaC-cRNA; (3) Dm-cRNA; (4) the mRNA of D.melanogaster culture cells and (5) the stable cytoplasmic poly (A) RNA from the same source [7].
• The genome of HiPV consists of 9,275 nucleotides excluding the poly (A) tail, and contains two large open reading frames (ORFs), which were separated by a 176-nucleotide noncoding region [8].
• The LN of poly (A) tracts present in whole embryos, larvae, pupae, and imagos is in each case close to 70 nucleotides [9].

Anatomical context of poly

• These nuclear transcripts are relatively small (7 - 12S), poly A(+) and appear on polysomes [10].
• Glucose is a poly-alcohol and, due to its high hydrophilicity, cannot move across cell membranes [11].
• Synthesis of RNA containing poly A in third instar salivary glands isolated nuclei [12].
• Nucleic acid reassociation methods were used to estimate the number of different polyadenylated RNA [poly (A) + RNA] sequences in the cytoplasm of whole Drosophila melanogaster at different stages of development and in the cytoplasm of cells of the L3 cell line [9].
• There, the gene is detected first when the myoepithelial cells of the polyp dedifferentiate to form the medusa bud and later Twist is found transiently in the entocodon, a mesoderm-like cell layer which differentiates into the smooth muscle and striated muscle of the bell [13].

Associations of poly with chemical compounds

• The inhibitor of poly ADP-ribosylation, 3-aminobenzamide (3AB), caused a pronounced increase of wing spots, induced by alkylating agents [14].
• Reassessment of the base specificity of RNase CL1 found that it is guanylic acid, then uridylic acid-preferential, and not poly U preferential [15].

Other interactions of poly

• The poly A containing ADH-mRNA has been purified by specific precipitation of ADH-polysomes using anti-ADH antibody and S. aureus [16].
• Replication of poly dA and poly rA by a drosophila DNA polymerase [17].
• By studying nos gene expression throughout the Podocoryne biphasic life cycle, we find that the germline differentiates exclusively during medusa development, whereas the polyp does not contribute to the process [18].

Analytical, diagnostic and therapeutic context of poly

• Sequence analysis reveals that Ic is the repeating heptanucleotide [poly d(AATATAG): d(CTATATT)] [19].
• Two loci on the X chromosome have been implicated in choriogenesis by in situ hybridization of poly A-containing RNA from choriogenic eggchambers to Drosophila polytene chromosomes (A.C. Spradling and A.P. Mahowald (1979). Cell 16, 589-598): 7E and 12E [20].

References