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Gene Review

RpII140  -  RNA polymerase II 140kD subunit

Drosophila melanogaster

Synonyms: CG3180, DNA-directed RNA polymerase II 140 kDa polypeptide, DNA-directed RNA polymerase II subunit RPB2, DmRP140, Dmel\CG3180, ...
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Disease relevance of RpII140


High impact information on RpII140

  • We conclude that C4 is an allele of the L5 locus and that this locus is most probably a structural gene for a subunit of RNA polymerase II [2].
  • Transcription factor IID, possibly with the cooperation of TFIIA, binds to the TATA element of the promoter, forming a complex that can bind TFIIB (refs 6, 7) followed by RNA polymerase II (refs 6, 8) and other factors [3].
  • In Drosophila and human cells, the TATA binding protein (TBP) of the transcription factor IID (TFIID) complex is tightly associated with multiple subunits termed TBP-associated factors (TAFs) that are essential for mediating regulation of RNA polymerase II transcription [4].
  • Sequence-specific antirepression of histone H1-mediated inhibition of basal RNA polymerase II transcription [5].
  • To understand the principles of control and selectivity in gene expression, the biochemical mechanisms by which promoter- and enhancer-binding factors regulate transcription by RNA polymerase II were analyzed [5].

Biological context of RpII140

  • Here, we show that upon heat shock the Pol II-free form of Mediator is rapidly recruited to HSF binding sites [6].
  • We have mapped a number of mutations at the DNA sequence level in genes encoding the largest (RpII215) and second-largest (RpII140) subunits of Drosophila melanogaster RNA polymerase II [7].
  • We show that Pcf11 is directly involved in termination in Drosophila. dPcf11 is concentrated at the 3' end of the hsp70 gene in cells, and depletion of dPcf11 with RNAi causes Pol II to readthrough the normal region of termination. dPcf11 also localizes to most transcribed loci on polytene chromosomes [8].
  • Core promoters for RNA polymerase II frequently contain either (or both) of two consensus sequence elements, a TATA box and/or an initiator (Inr) [9].
  • DNA sequence analysis of RpII215, the gene that encodes the Mr215,000 subunit of RNA polymerase II (EC in Drosophila melanogaster, reveals that the 3'-terminal exon includes a region encoding a C-terminal domain composed of 42 repeats of a seven-residue amino acid consensus sequence, Tyr-Ser-Pro-Thr-Ser-Pro-Ser [10].

Anatomical context of RpII140


Associations of RpII140 with chemical compounds


Other interactions of RpII140


Analytical, diagnostic and therapeutic context of RpII140

  • Focusing on RpII140, we used in situ hybridization to map this gene to a small subinterval defined by the endpoints of a series of deficiencies impinging on the 88A/B region and showed that it does not represent a previously known genetic locus [14].
  • The chromatin immunoprecipitation assays revealed that HDI treatments induced the hyperacetylation of histone H3 at the promoter and the transcribing regions of hsp70 gene, increased the accessibility of heat-shock factor to target heat-shock element, and promoted the RNA polymerase II-mediated transcription [17].


  1. RNA polymerase II of Drosophila. Relation of its 140,000 Mr subunit to the beta subunit of Escherichia coli RNA polymerase. Falkenburg, D., Dworniczak, B., Faust, D.M., Bautz, E.K. J. Mol. Biol. (1987) [Pubmed]
  2. Genetic and biochemical characterization of mutants at an RNA polymerase II locus in D. melanogaster. Greenleaf, A.L., Weeks, J.R., Voelker, R.A., Ohnishi, S., Dickson, B. Cell (1980) [Pubmed]
  3. Interaction between a transcriptional activator and transcription factor IIB in vivo. Colgan, J., Wampler, S., Manley, J.L. Nature (1993) [Pubmed]
  4. Drosophila TAFII150: similarity to yeast gene TSM-1 and specific binding to core promoter DNA. Verrijzer, C.P., Yokomori, K., Chen, J.L., Tjian, R. Science (1994) [Pubmed]
  5. Sequence-specific antirepression of histone H1-mediated inhibition of basal RNA polymerase II transcription. Croston, G.E., Kerrigan, L.A., Lira, L.M., Marshak, D.R., Kadonaga, J.T. Science (1991) [Pubmed]
  6. Mediator, not holoenzyme, is directly recruited to the heat shock promoter by HSF upon heat shock. Park, J.M., Werner, J., Kim, J.M., Lis, J.T., Kim, Y.J. Mol. Cell (2001) [Pubmed]
  7. Mapping mutations in genes encoding the two large subunits of Drosophila RNA polymerase II defines domains essential for basic transcription functions and for proper expression of developmental genes. Chen, Y., Weeks, J., Mortin, M.A., Greenleaf, A.L. Mol. Cell. Biol. (1993) [Pubmed]
  8. Pcf11 is a termination factor in Drosophila that dismantles the elongation complex by bridging the CTD of RNA polymerase II to the nascent transcript. Zhang, Z., Gilmour, D.S. Mol. Cell (2006) [Pubmed]
  9. Cooperation between core promoter elements influences transcriptional activity in vivo. Colgan, J., Manley, J.L. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  10. The C-terminal repeat domain of RNA polymerase II largest subunit is essential in vivo but is not required for accurate transcription initiation in vitro. Zehring, W.A., Lee, J.M., Weeks, J.R., Jokerst, R.S., Greenleaf, A.L. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  11. TATA box-binding protein (TBP)-related factor 2 (TRF2), a third member of the TBP family. Rabenstein, M.D., Zhou, S., Lis, J.T., Tjian, R. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  12. Identification and purification of a yeast protein that affects elongation by RNA polymerase II. Chafin, D.R., Claussen, T.J., Price, D.H. J. Biol. Chem. (1991) [Pubmed]
  13. Stability of Drosophila RNA polymerase II elongation complexes in vitro. Kephart, D.D., Marshall, N.F., Price, D.H. Mol. Cell. Biol. (1992) [Pubmed]
  14. Reverse genetics of Drosophila RNA polymerase II: identification and characterization of RpII140, the genomic locus for the second-largest subunit. Hamilton, B.J., Mortin, M.A., Greenleaf, A.L. Genetics (1993) [Pubmed]
  15. Structure of the genes encoding transcription factor IIB and TATA box-binding protein from Drosophila melanogaster. Lira-DeVito, L.M., Burke, T.W., Kadonaga, J.T. Gene (1995) [Pubmed]
  16. Even-skipped represses transcription by binding TATA binding protein and blocking the TFIID-TATA box interaction. Li, C., Manley, J.L. Mol. Cell. Biol. (1998) [Pubmed]
  17. Effects of histone deacetylase inhibitors on transcriptional regulation of the hsp70 gene in Drosophila. Zhao, Y.M., Chen, X., Sun, H., Yuan, Z.G., Ren, G.L., Li, X.X., Lu, J., Huang, B.Q. Cell Res. (2006) [Pubmed]
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