Disease relevance of MUC3B

• The role of MUC1 and MUC3 in the biology and prognosis of colorectal cancer [1].
• In exploration of the mechanism of hypoxic regulation of mucin 3 expression, we demonstrated by luciferase assay that the full-length promoter for mouse Mucin 3 (Muc3) is hypoxia-responsive with a 5.08 +/- 1.76-fold induction following 24 h of hypoxia [2].

High impact information on MUC3B

• Cysteine-rich domains of muc3 intestinal mucin promote cell migration, inhibit apoptosis, and accelerate wound healing [3].
• Endogenous MUC3A in LoVo was inhibited by specific small interfering RNA transfection [3].
• In contrast, MUC2 mRNA was expressed at only low levels and MUC3 was not detected in the pancreatic tumor cell lines [4].
• No alteration in MUC3 or MUC4 gene expression was detectable in UC compared with normal colorectal mucosa [5].
• ITF not only colocalizes with MUC3, by confocal microscopy, to the apical surface of T84 cells following exposure to hypoxia, but is also found, by co-immunoprecipitation, to be physically associated with MUC3, following 24 h of hypoxia [2].

Anatomical context of MUC3B

• Concomitantly MUC3 protein precursor (approximately 550 kDa) was detected in Caco-2 cells [6].
• Furthermore, MUC3 mRNA is expressed in a differentiation dependent manner, as is the case for enterocytes [6].
• However, MUC3 mRNA expression increased in the ileum and colon of these rats [7].
• We demonstrate by PCR confirmation of microarray data and by avidin blotting of immunoprecipitated human Mucin 3 (MUC3), that surface MUC3 expression is induced in T84 intestinal epithelial cells following exposure to hypoxia [2].

Associations of MUC3B with chemical compounds

• MUC2 mRNA levels were decreased in cells grown in galactose medium compared to glucose medium whereas MUC3 mRNA levels were increased when cells were grown in galactose medium compared to those grown in galactose-free, glucose medium [8].

Analytical, diagnostic and therapeutic context of MUC3B

• We also utilized 5'-GeneRacer PCR to determine the sequence of the 5'-terminus of the MUC3B message [9].
• Northern blot analyses using cDNA probes of MUC2 and MUC3 were performed [8].
• Nuclear factor (NF)-kappaB activation, interleukin (IL)-8 secretion, pathogen adherence to IECs, and mucin-3 (MUC3) and E-cadherin gene expression were assayed by TransAM assay, enzyme-linked immunosorbent assay (ELISA), fluorescence, and real-time reverse transcriptase-polymerase chain reaction (RT-PCR), respectively [10].

References