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Gene Review

Slc9a3r1  -  solute carrier family 9, subfamily A (NHE3...

Rattus norvegicus

Synonyms: EBP50, Ezrin-radixin-moesin-binding phosphoprotein 50, NHERF-1, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Nherf, ...
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Disease relevance of Slc9a3r1


High impact information on Slc9a3r1

  • The present studies evaluated the comparative expression and localization of EBP50 and E3KARP in rat hepatocytes and cholangiocytes [1].
  • Complementary DNAs encoding rat EBP50 and E3KARP were identified by reverse transcription-polymerase chain reaction in both epithelial cell types and subsequently sequenced [1].
  • ERM family members serve to anchor EBP50 and E3KARP to the actin cytoskeleton and sequester protein kinase A (PKA) to these protein complexes [1].
  • Ezrin-radixin-moesin (ERM)-binding phosphoprotein 50 (EBP50) and NHE3 Kinase A regulatory protein (E3KARP) are membrane-cytoskeleton linking proteins that utilize 2 PSD-95/DIg/ZO-1 (PDZ) domains and an ERM binding site to coordinate cyclic adenosine monophosphate (cAMP)-regulated ion transport in a number of distinct epithelia [1].
  • 6. In the presence of NHERF-1, the ASIC3 subcellular localization is modified, and the channel co-localizes with ezrin, a member of the ezrin-radixin-moesin family of actin-binding proteins, providing the first direct link between ASIC3 and the cortical cytoskeleton [2].

Biological context of Slc9a3r1

  • These EBP50 binding interactions have been implicated in sequestering interactive sets of proteins into common microdomains, regulating the activity of interacting proteins, and modulating membrane protein trafficking [3].
  • EBP50 acts via multiple protein binding interactions, including oligomerization through interactions of its PSD95-Dlg-ZO1 (PDZ) domains [4].
  • EBP50 can be phosphorylated on multiple sites and phosphorylation of specific sites modulates the extent of oligomerization [4].
  • In vitro experiments showed that the catalytic subunit of PKC directly phosphorylates EBP50 [4].

Anatomical context of Slc9a3r1

  • Hypothesizing that EBP50 self-associates to amplify its functional capacity, far-Western blotting of cholangiocyte epithelial cell proteins with EBP50 fusion protein revealed that EBP50 binds to a 50-kDa protein [3].
  • Given the importance of the ASIC3 sustained current in nociceptor excitability, it is likely that NHERF-1 participates in channel functions associated with nociception and mechanosensation [2].
  • NHERF-1 enhances the ASIC3 peak current in heterologous cells, including F-11 dorsal root ganglion cells, by increasing the amount of channel at the plasma membrane [2].
  • The effects of microtubule disruption by colchicine on NHE3 trafficking in PT and the potential role of NHERF in this process have not been studied [5].

Associations of Slc9a3r1 with chemical compounds

  • In HEK-293 cells transfected with rat EBP50 cDNA, a treatment with 12 myristate 13-acetate (PMA) induced a translocation of PKCalpha and beta isoforms to the membrane and increased 32P incorporation into EBP50 [4].
  • Replacement of the COOH-terminal leucine with glycine blocked binding of both the proton pump subunits but did not affect binding of NHERF-1 [6].
  • With the use of antibodies specific for NHERF and NHERF2, immunocytochemical analysis of rat kidney was undertaken to determine the nephron distribution of both proteins and their colocalization with other transporters and with ezrin [7].

Other interactions of Slc9a3r1


Analytical, diagnostic and therapeutic context of Slc9a3r1


  1. Ezrin-radixin-moesin-binding phosphoprotein 50 is expressed at the apical membrane of rat liver epithelia. Fouassier, L., Duan, C.Y., Feranchak, A.P., Yun, C.H., Sutherland, E., Simon, F., Fitz, J.G., Doctor, R.B. Hepatology (2001) [Pubmed]
  2. Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1. Deval, E., Friend, V., Thirant, C., Salinas, M., Jodar, M., Lazdunski, M., Lingueglia, E. J. Biol. Chem. (2006) [Pubmed]
  3. Evidence for ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) self-association through PDZ-PDZ interactions. Fouassier, L., Yun, C.C., Fitz, J.G., Doctor, R.B. J. Biol. Chem. (2000) [Pubmed]
  4. Protein kinase C regulates the phosphorylation and oligomerization of ERM binding phosphoprotein 50. Fouassier, L., Nichols, M.T., Gidey, E., McWilliams, R.R., Robin, H., Finnigan, C., Howell, K.E., Housset, C., Doctor, R.B. Exp. Cell Res. (2005) [Pubmed]
  5. NHE3 and NHERF are targeted to the basolateral membrane in proximal tubules of colchicine-treated rats. Sabolić, I., Herak-Kramberger, C.M., Ljubojević, M., Biemesderfer, D., Brown, D. Kidney Int. (2002) [Pubmed]
  6. The COOH termini of NBC3 and the 56-kDa H+-ATPase subunit are PDZ motifs involved in their interaction. Pushkin, A., Abuladze, N., Newman, D., Muronets, V., Sassani, P., Tatishchev, S., Kurtz, I. Am. J. Physiol., Cell Physiol. (2003) [Pubmed]
  7. Differential renal distribution of NHERF isoforms and their colocalization with NHE3, ezrin, and ROMK. Wade, J.B., Welling, P.A., Donowitz, M., Shenolikar, S., Weinman, E.J. Am. J. Physiol., Cell Physiol. (2001) [Pubmed]
  8. Expression of TRPC4 channel protein that interacts with NHERF-2 in rat descending vasa recta. Lee-Kwon, W., Wade, J.B., Zhang, Z., Pallone, T.L., Weinman, E.J. Am. J. Physiol., Cell Physiol. (2005) [Pubmed]
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