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Gene Review

Clcnka  -  chloride channel, voltage-sensitive Ka

Rattus norvegicus

Synonyms: Chloride channel Ka, Chloride channel protein ClC-Ka, ClC-K1, Clcnk1
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Disease relevance of Clcnk1


High impact information on Clcnk1

  • The localization and the functional characteristics described here indicate that ClC-K1 is responsible for the transepithelial chloride transport in tAL [2].
  • Localization and functional characterization of rat kidney-specific chloride channel, ClC-K1 [2].
  • Immunoelectron microscopy confirmed that the staining of ClC-K1 in tAL was observed in the region of both apical and basolateral plasma membranes [2].
  • These results suggest that ClC-K2L and -K2S are chloride channels in the thick ascending limb and collecting ducts and may be important routes for transcellular chloride transport like ClC-K1 [3].
  • We conclude that the descending thin limbs shift from expressing AQP1 to expressing ClC-K1 some distance before the point where they turn and begin to ascend [4].

Biological context of Clcnk1


Anatomical context of Clcnk1

  • To investigate the physiological role of a kidney-specific chloride channel (ClC-K1), we sought to determine its exact localization by immunohistochemistry and its functional regulation using Xenopus oocyte expression system [2].
  • mRNA encoding 'ClC-K1, a kidney Cl(-)- channel' is expressed in marginal cells of the stria vascularis of rat cochlea: its possible contribution to Cl(-) currents [7].
  • Thus these results demonstrate that rat kidney ClC-K channels are predominantly located in the basolateral membranes from cells of the late segments of the renal tubule where most of chloride reabsorption takes place [1].

Associations of Clcnk1 with chemical compounds


Regulatory relationships of Clcnk1


Other interactions of Clcnk1

  • Localisation and regulation of ClC-K1 and barttin mRNA was analysed by RNase protection and in situ hybridisation [5].
  • The 687-amino acid protein encoded by ClC-K2L is about 80% identical to rat ClC-K1 and about 40% identical to ClC-0, -1, and -2 [3].
  • At each level sampled in the inner medulla, many more thin limbs were labeled by ClC-K1 than AQP1 [4].
  • We have isolated protein from the gerbil kidney, stria vascularis and vestibular labyrinth and found by Western blot analysis a 60 kDa band, a 48 kDa band and 54 and 70 kDa bands, respectively, specifically labeled by ClC-K antibody [8].

Analytical, diagnostic and therapeutic context of Clcnk1


  1. Localization and induction by dehydration of ClC-K chloride channels in the rat kidney. Vandewalle, A., Cluzeaud, F., Bens, M., Kieferle, S., Steinmeyer, K., Jentsch, T.J. Am. J. Physiol. (1997) [Pubmed]
  2. Localization and functional characterization of rat kidney-specific chloride channel, ClC-K1. Uchida, S., Sasaki, S., Nitta, K., Uchida, K., Horita, S., Nihei, H., Marumo, F. J. Clin. Invest. (1995) [Pubmed]
  3. Two isoforms of a chloride channel predominantly expressed in thick ascending limb of Henle's loop and collecting ducts of rat kidney. Adachi, S., Uchida, S., Ito, H., Hata, M., Hiroe, M., Marumo, F., Sasaki, S. J. Biol. Chem. (1994) [Pubmed]
  4. Immunomorphometric study of rat renal inner medulla. Mejia, R., Wade, J.B. Am. J. Physiol. Renal Physiol. (2002) [Pubmed]
  5. Parallel down-regulation of chloride channel CLC-K1 and barttin mRNA in the thin ascending limb of the rat nephron by furosemide. Wolf, K., Meier-Meitinger, M., Bergler, T., Castrop, H., Vitzthum, H., Riegger, G.A., Kurtz, A., Krämer, B.K. Pflugers Arch. (2003) [Pubmed]
  6. Barttin increases surface expression and changes current properties of ClC-K channels. Waldegger, S., Jeck, N., Barth, P., Peters, M., Vitzthum, H., Wolf, K., Kurtz, A., Konrad, M., Seyberth, H.W. Pflugers Arch. (2002) [Pubmed]
  7. mRNA encoding 'ClC-K1, a kidney Cl(-)- channel' is expressed in marginal cells of the stria vascularis of rat cochlea: its possible contribution to Cl(-) currents. Ando, M., Takeuchi, S. Neurosci. Lett. (2000) [Pubmed]
  8. Immunolocalization of ClC-K chloride channel in strial marginal cells and vestibular dark cells. Sage, C.L., Marcus, D.C. Hear. Res. (2001) [Pubmed]
  9. ClC family in the kidney. Sasaki, S., Uchida, S., Kawasaki, M., Adachi, S., Marumo, F. Jpn. J. Physiol. (1994) [Pubmed]
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