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Gene Review

IDP2  -  isocitrate dehydrogenase (NADP(+)) IDP2

Saccharomyces cerevisiae S288c

Synonyms: IDH, IDP, L9470.12, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, ...
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High impact information on IDP2

  • To compare kinetic properties of homologous isozymes of NADP+-specific isocitrate dehydrogenase, histidine-tagged forms of yeast mitochondrial (IDP1) and cytosolic (IDP2) enzymes were expressed and purified [1].
  • Similar Km values for substrates and cofactors in decarboxylation and carboxylation reactions were obtained for IDP2, suggesting a capacity for bidirectional catalysis in vivo [1].
  • For mutant strains lacking IDP1, IDP2, and/or the mitochondrial NAD+-specific isocitrate dehydrogenase (IDH), metabolite measurements indicated that major cellular flux is through the IDH-catalyzed reaction in glucose-grown cells and through the IDP2-catalyzed reaction in cells grown with a nonfermentable carbon source (glycerol and lactate) [1].
  • However, we have found that a zwf1Delta ald6Delta mutant can be constructed by mating when tetrads are dissected on plates with a nonfermentable carbon source (lactate), a condition associated with expression of another enzymatic source of NADPH, cytosolic NADP+-specific isocitrate dehydrogenase (Idp2p) [2].
  • This mutation, an insertion of a Ty delta element into the 5' region of IDP2, markedly elevates expression of Idp2p in glucose media [3].

Biological context of IDP2


Associations of IDP2 with chemical compounds

  • These include reduced growth in the absence of glutamate, as well as an increase in expression of Idp2p (the cytosolic NADP+-dependent enzyme) under some growth conditions [8].
  • Cat8p, the activator of gluconeogenic genes in Saccharomyces cerevisiae, regulates carbon source-dependent expression of NADP-dependent cytosolic isocitrate dehydrogenase (Idp2p) and lactate permease (Jen1p) [9].
  • Instead, we propose that the common function shared by IDP2 and ZWF1 is maintenance of significant levels of NADPH for enzymatic removal of the hydrogen peroxide generated in the first step of peroxisomal beta-oxidation in yeast and that inadequate levels of the reduced form of the cofactor can produce lethality [10].
  • The mammalian cytosolic isozyme was found to partition between cytosolic and organellar compartments and to replace functionally Idp2p for production of alpha-ketoglutarate or for growth on fatty acids in a mutant lacking Zwf1p [11].

Other interactions of IDP2

  • Loss of Idp1p (the mitochondrial NADP+-dependent enzyme) and Idp2p contributes to the loss of functional mtDNA, but only in an IDH dysfunctional background [8].
  • Collectively, these results suggest that IDP2, when expressed, and ZWF1 have critical overlapping functions in provision of reducing equivalents for defense against endogenous or exogenous sources of H2O2 [10].


  1. Kinetic properties and metabolic contributions of yeast mitochondrial and cytosolic NADP+-specific isocitrate dehydrogenases. Contreras-Shannon, V., Lin, A.P., McCammon, M.T., McAlister-Henn, L. J. Biol. Chem. (2005) [Pubmed]
  2. Sources of NADPH in yeast vary with carbon source. Minard, K.I., McAlister-Henn, L. J. Biol. Chem. (2005) [Pubmed]
  3. The aconitase function of iron regulatory protein 1. Genetic studies in yeast implicate its role in iron-mediated redox regulation. Narahari, J., Ma, R., Wang, M., Walden, W.E. J. Biol. Chem. (2000) [Pubmed]
  4. Influence of compartmental localization on the function of yeast NADP+-specific isocitrate dehydrogenases. Contreras-Shannon, V., McAlister-Henn, L. Arch. Biochem. Biophys. (2004) [Pubmed]
  5. Function and expression of yeast mitochondrial NAD- and NADP-specific isocitrate dehydrogenases. Haselbeck, R.J., McAlister-Henn, L. J. Biol. Chem. (1993) [Pubmed]
  6. Antioxidant function of cytosolic sources of NADPH in yeast. Minard, K.I., McAlister-Henn, L. Free Radic. Biol. Med. (2001) [Pubmed]
  7. Isolation, characterization, and disruption of the yeast gene encoding cytosolic NADP-specific isocitrate dehydrogenase. Loftus, T.M., Hall, L.V., Anderson, S.L., McAlister-Henn, L. Biochemistry (1994) [Pubmed]
  8. Multiple cellular consequences of isocitrate dehydrogenase isozyme dysfunction. McCammon, M.T., McAlister-Henn, L. Arch. Biochem. Biophys. (2003) [Pubmed]
  9. Cat8p, the activator of gluconeogenic genes in Saccharomyces cerevisiae, regulates carbon source-dependent expression of NADP-dependent cytosolic isocitrate dehydrogenase (Idp2p) and lactate permease (Jen1p). Bojunga, N., Entian, K.D. Mol. Gen. Genet. (1999) [Pubmed]
  10. Dependence of peroxisomal beta-oxidation on cytosolic sources of NADPH. Minard, K.I., McAlister-Henn, L. J. Biol. Chem. (1999) [Pubmed]
  11. Sources of NADPH and expression of mammalian NADP+-specific isocitrate dehydrogenases in Saccharomyces cerevisiae. Minard, K.I., Jennings, G.T., Loftus, T.M., Xuan, D., McAlister-Henn, L. J. Biol. Chem. (1998) [Pubmed]
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