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Gene Review

IME1  -  Ime1p

Saccharomyces cerevisiae S288c

Synonyms: J1916, Meiosis-inducing protein 1, YJR094C
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High impact information on IME1

  • (1988) found that overexpression of IME1 allowed sporulation in the presence of glucose and nitrogen [1].
  • Thus, while it is clear that IME1 is a necessary positive regulator of meiosis, responding both to mating type and nutritional conditions, it is not clear if it is sufficient [1].
  • We conclude that IME1 is a positive regulator of meiosis that normally is repressed by RME1 [2].
  • Starvation for nitrogen further induced (6- to 8-fold) transcription of IME1, but, as expected, the induction was found only in MATa/MAT alpha or rme1-1/rme1-1 diploids [2].
  • IME1 (Inducer of MEiosis) was cloned due to its high copy number effect: it enabled MAT insufficient strains to undergo meiosis [2].

Biological context of IME1

  • The gene IME1 is also involved in starvation control of meiosis [3].
  • Moreover, the addition of cAMP to a cyr1-2 diploid suppresses IME1 transcription [3].
  • Multicopy IME1 plasmids overcome the meiotic deficiency of bcy1 and of RASval19 diploids [3].
  • This indicates that the pathway downstream of the AC/PK cascade branches to control meiosis through IME1, and to control entry into G0 and cell cycle initiation, independently of IME1 [3].
  • Thus, in wild-type strains, the increased expression of IME1 in starved a/alpha cells can account entirely for cell type control, but only partially for nutritional control, of early sporulation-specific gene expression [4].

Associations of IME1 with chemical compounds

  • The deduced IME1 product is a 360-amino-acid protein with a tyrosine-rich C-terminal region [4].
  • BACKGROUND: IME1, which is required for the initiation of meiosis, is regulated by Cln3:Cdc28 kinase, which activates the G1-to-S transition, and Snf1 kinase, which mediates glucose repression [5].
  • We further show that this nutrient signal is transmitted to Rim11 and consequently to Ime1 by the cyclic AMP/protein kinase A signal transduction pathway [6].
  • On the contrary, the activation of TOR by glutamine induces the relocalization of Ime1 to the cytoplasm [7].
  • Ime1 is a transcriptional activator that is required for transcription of IME2, a serine/threonine protein kinase [8].

Physical interactions of IME1

  • We show that Rme1p binds to two sites in the IME1 upstream region, near nt -1950 and -2030 [9].
  • YHP1 encodes a new homeoprotein that binds to the IME1 promoter in Saccharomyces cerevisiae [10].
  • Here, we characterize mutant Ime1p derivatives that interact with Rim11p but are not phosphorylated in vitro [11].
  • By engineering constructs which express various portions of the Ime1p fused to either the DNA binding or transcriptional activation domains of GAL4, we have conclusively demonstrated that IME1 is a transcription factor, apparently required for sporulation to activate the transcription of meiosis specific genes [12].

Enzymatic interactions of IME1

  • In addition, HA-RIM11 immune complexes phosphorylate a functional IME1 derivative but not the corresponding ime1-L321F derivative [13].
  • These results argue that Ime1p must be phosphorylated to interact with Ume6p [11].

Regulatory relationships of IME1


Other interactions of IME1

  • This observation indicates that MCK1 is required, independently, for both the activation of IME1 and subsequent ascus maturation [17].
  • We report here an analysis of the regulatory region of IME2, an IME1-dependent meiotic gene [18].
  • We propose that IME1 modifies UME6 to convert it from a negulator to a positive Regulor [18].
  • Analysis of RIM11, a yeast protein kinase that phosphorylates the meiotic activator IME1 [13].
  • Two-hybrid interaction assays suggest that Ime1p-Ume6p interaction is diminished in a rim15 mutant [19].

Analytical, diagnostic and therapeutic context of IME1


  1. Dual regulation of meiosis in yeast. Malone, R.E. Cell (1990) [Pubmed]
  2. IME1, a positive regulator gene of meiosis in S. cerevisiae. Kassir, Y., Granot, D., Simchen, G. Cell (1988) [Pubmed]
  3. The adenylate cyclase/protein kinase cascade regulates entry into meiosis in Saccharomyces cerevisiae through the gene IME1. Matsuura, A., Treinin, M., Mitsuzawa, H., Kassir, Y., Uno, I., Simchen, G. EMBO J. (1990) [Pubmed]
  4. Role of IME1 expression in regulation of meiosis in Saccharomyces cerevisiae. Smith, H.E., Su, S.S., Neigeborn, L., Driscoll, S.E., Mitchell, A.P. Mol. Cell. Biol. (1990) [Pubmed]
  5. The CLN3/SWI6/CLN2 pathway and SNF1 act sequentially to regulate meiotic initiation in Saccharomyces cerevisiae. Purnapatre, K., Piccirillo, S., Schneider, B.L., Honigberg, S.M. Genes Cells (2002) [Pubmed]
  6. The in vivo activity of Ime1, the key transcriptional activator of meiosis-specific genes in Saccharomyces cerevisiae, is inhibited by the cyclic AMP/protein kinase A signal pathway through the glycogen synthase kinase 3-beta homolog Rim11. Rubin-Bejerano, I., Sagee, S., Friedman, O., Pnueli, L., Kassir, Y. Mol. Cell. Biol. (2004) [Pubmed]
  7. TOR regulates the subcellular localization of Ime1, a transcriptional activator of meiotic development in budding yeast. Colomina, N., Liu, Y., Aldea, M., Garí, E. Mol. Cell. Biol. (2003) [Pubmed]
  8. Ime2, a meiosis-specific kinase in yeast, is required for destabilization of its transcriptional activator, Ime1. Guttmann-Raviv, N., Martin, S., Kassir, Y. Mol. Cell. Biol. (2002) [Pubmed]
  9. Genomic footprinting of the yeast zinc finger protein Rme1p and its roles in repression of the meiotic activator IME1. Shimizu, M., Li, W., Covitz, P.A., Hara, M., Shindo, H., Mitchell, A.P. Nucleic Acids Res. (1998) [Pubmed]
  10. YHP1 encodes a new homeoprotein that binds to the IME1 promoter in Saccharomyces cerevisiae. Kunoh, T., Kaneko, Y., Harashima, S. Yeast (2000) [Pubmed]
  11. Catalytic roles of yeast GSK3beta/shaggy homolog Rim11p in meiotic activation. Malathi, K., Xiao, Y., Mitchell, A.P. Genetics (1999) [Pubmed]
  12. IME1 gene encodes a transcription factor which is required to induce meiosis in Saccharomyces cerevisiae. Mandel, S., Robzyk, K., Kassir, Y. Dev. Genet. (1994) [Pubmed]
  13. Analysis of RIM11, a yeast protein kinase that phosphorylates the meiotic activator IME1. Bowdish, K.S., Yuan, H.E., Mitchell, A.P. Mol. Cell. Biol. (1994) [Pubmed]
  14. Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast. Benni, M.L., Neigeborn, L. Genetics (1997) [Pubmed]
  15. The Tup1-Ssn6 general repressor is involved in repression of IME1 encoding a transcriptional activator of meiosis in Saccharomyces cerevisiae. Mizuno, T., Nakazawa, N., Remgsamrarn, P., Kunoh, T., Oshima, Y., Harashima, S. Curr. Genet. (1998) [Pubmed]
  16. A transcriptional cascade governs entry into meiosis in Saccharomyces cerevisiae. Smith, H.E., Mitchell, A.P. Mol. Cell. Biol. (1989) [Pubmed]
  17. The yeast MCK1 gene encodes a protein kinase homolog that activates early meiotic gene expression. Neigeborn, L., Mitchell, A.P. Genes Dev. (1991) [Pubmed]
  18. Bipartite structure of an early meiotic upstream activation sequence from Saccharomyces cerevisiae. Bowdish, K.S., Mitchell, A.P. Mol. Cell. Biol. (1993) [Pubmed]
  19. Stimulation of yeast meiotic gene expression by the glucose-repressible protein kinase Rim15p. Vidan, S., Mitchell, A.P. Mol. Cell. Biol. (1997) [Pubmed]
  20. Multiple and distinct activation and repression sequences mediate the regulated transcription of IME1, a transcriptional activator of meiosis-specific genes in Saccharomyces cerevisiae. Sagee, S., Sherman, A., Shenhar, G., Robzyk, K., Ben-Doy, N., Simchen, G., Kassir, Y. Mol. Cell. Biol. (1998) [Pubmed]
  21. Dissection of the DNA binding domain of yeast Zn-finger protein Rme1p, a repressor of meiotic activator IME1. Shimizu, M., Hara, M., Murase, A., Shindo, H., Mitchell, A.P. Nucleic Acids Symp. Ser. (1997) [Pubmed]
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