The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

MET14  -  adenylyl-sulfate kinase

Saccharomyces cerevisiae S288c

Synonyms: APS kinase, ATP adenosine-5'-phosphosulfate 3'-phosphotransferase, Adenosine-5'-phosphosulfate kinase, Adenylyl-sulfate kinase, YKL001C
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of MET14


High impact information on MET14


Biological context of MET14

  • Two strains with low sulphite production were transformed with high-copy plasmids containing either or both MET14 and MET16 [6].
  • Large (10.5-13.5 kbp) circular minichromosomes containing the centromere of chromosome 11 (CEN11) and the MET14 gene of Saccharomyces cerevisiae in the YRp7 vector are considerably more stable during mitosis than smaller ones containing only the 1.6 kbp CEN11 SalI-fragment [7].
  • The cumulative results suggest that (a) the allosteric PAPS binding site of P. chrysogenum ATP sulfurylase is located in the C-terminal domain of the protein and (b) that this domain may have evolved from APS kinase [1].
  • Cloning, nucleotide sequence, and regulation of MET14, the gene encoding the APS kinase of Saccharomyces cerevisiae [8].
  • Using a yeast two-hybrid system with AtAkn1 as bait, an interacting clone was detected from a cDNA library of A. thaliana cv. Columbia that codes for an APS-kinase iso-form (Atakn2) [9].

Associations of MET14 with chemical compounds

  • The level of MET14- and MET16-mRNA varied with sulphite production, whereas the level of MET3-mRNA was very weak in almost all strains [6].
  • Furthermore, in the 270 bp upstream of the MET14 coding region there are several matches with a methionine-specific upstream negative (URSMet) control element [8].
  • The addition of glucose can also increase the sulphite formation in strains overexpressing MET14 and/or SSU1 under oxygen-limiting conditions, while the addition of glucose has no significant effect under aerobic conditions [6].
  • We report here that not only the glutathione synthesis gene (GSH1) but also almost all transcripts of the enzymes involved in the sulfur amino acid metabolism, especially MET14 and MET17, were greatly induced after exposure to cadmium [10].
  • MET14, a gene important for sulfite formation, was overexpressed in wort, using the HSP26 promoter during the stationary phase [11].

Other interactions of MET14


Analytical, diagnostic and therapeutic context of MET14


  1. Cloning and sequencing of ATP sulfurylase from Penicillium chrysogenum. Identification of a likely allosteric domain. Foster, B.A., Thomas, S.M., Mahr, J.A., Renosto, F., Patel, H.C., Segel, I.H. J. Biol. Chem. (1994) [Pubmed]
  2. Molecular genetics of sulfur assimilation in filamentous fungi and yeast. Marzluf, G.A. Annu. Rev. Microbiol. (1997) [Pubmed]
  3. Direct selection procedure for the isolation of functional centromeric DNA. Hsiao, C.L., Carbon, J. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  4. Isolation and subcloning analysis of functional centromere DNA (CEN11) from Saccharomyces cerevisiae chromosome XI. Fitzgerald-Hayes, M., Buhler, J.M., Cooper, T.G., Carbon, J. Mol. Cell. Biol. (1982) [Pubmed]
  5. Nuclear localization of PAPS synthetase 1: a sulfate activation pathway in the nucleus of eukaryotic cells. Besset, S., Vincourt, J.B., Amalric, F., Girard, J.P. FASEB J. (2000) [Pubmed]
  6. Increasing sulphite formation in Saccharomyces cerevisiae by overexpression of MET14 and SSU1. Donalies, U.E., Stahl, U. Yeast (2002) [Pubmed]
  7. Structure and mitotic stability of minichromosomes originating in yeast cells transformed with tandem dimers of CEN11 plasmids. Oertel, W., Mayer, M. Mol. Gen. Genet. (1984) [Pubmed]
  8. Cloning, nucleotide sequence, and regulation of MET14, the gene encoding the APS kinase of Saccharomyces cerevisiae. Korch, C., Mountain, H.A., Byström, A.S. Mol. Gen. Genet. (1991) [Pubmed]
  9. Molecular and catalytic properties of Arabidopsis thaliana adenylyl sulfate (APS)-kinase. Lillig, C.H., Schiffmann, S., Berndt, C., Berken, A., Tischka, R., Schwenn, J.D. Arch. Biochem. Biophys. (2001) [Pubmed]
  10. Bioassay of cadmium using a DNA microarray: genome-wide expression patterns of Saccharomyces cerevisiae response to cadmium. Momose, Y., Iwahashi, H. Environ. Toxicol. Chem. (2001) [Pubmed]
  11. Phase-specific gene expression in Saccharomyces cerevisiae, using maltose as carbon source under oxygen-limiting conditions. Donalies, U.E., Stahl, U. Curr. Genet. (2001) [Pubmed]
  12. Direct photoaffinity labeling of proteins with adenosine 3'-[32P]phosphate 5'-phosphosulfate. Atractyloside inhibits labeling of a Mr = 34,000 protein in an adrenal medullary Golgi fraction. Lee, R.W., Suchanek, C., Huttner, W.B. J. Biol. Chem. (1984) [Pubmed]
WikiGenes - Universities