Disease relevance of HTZ1

• Nonperoxisomal processes, among them chromatin modification by H2AZ, Pol II mediator function, and cell-wall-associated activities, also prevent oleate toxicity [1].

High impact information on HTZ1

• Specific Htz1 deposition requires the SWR1 complex, which largely colocalizes with Htz1 [2].
• Furthermore, Htz1 promotes full gene activation but does not generally impact repression [2].
• Microarray analysis revealed that HTZ1-activated genes cluster near telomeres [3].
• Other Htz1-activated genes flank the silent HMR mating-type locus [3].
• Htz1 is enriched in these euchromatic regions and acts synergistically with a boundary element to prevent the spread of heterochromatin [3].

Biological context of HTZ1

• Importantly, fusion of the H2A.Z C-terminal region to S phase H2A without its corresponding C-terminal region can mediate the variant histone's specialized function in GAL1-10 gene induction, and it restores the slow-growth phenotype of cells with a deletion of HTZ1 [4].
• Using plasmid shuffle experiments, we show that the major H2A genes cannot provide the function of HTZ1 and the HTZ1 gene cannot provide the essential function of the genes encoding the major H2As [5].
• Acetylation of H2AZ Lys 14 is associated with genome-wide gene activity in yeast [6].
• Moreover, like Htz1 and Bdf1, the SWR-C promotes gene expression near silent heterochromatin [7].
• The histone H2A variant H2A.Z (Saccharomyces cerevisiae Htz1) plays roles in transcription, DNA repair, chromosome stability, and limiting telomeric silencing [8].

Associations of HTZ1 with chemical compounds

• We show here that, while the HTZ1 gene encoding H2A.Z is not essential in budding yeast, its disruption results in slow growth and formamide sensitivity [5].

Physical interactions of HTZ1

• We discovered that Swc2 binds directly to and is essential for transfer of H2AZ [9].

Enzymatic interactions of HTZ1

• ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex [10].

Other interactions of HTZ1

• In support of our genome-wide analysis, we found that the acetylatable lysines of Htz1 are required for its full deposition during nucleosome reassembly upon repression of PHO5 [6].
• The data presented here demonstrate that while Htz1 is associated preferentially with the promoters of repressed genes, K14 acetylation is enriched at the promoters of active genes, and requires two known histone acetyltransferases, Gcn5 and Esa1 [6].
• A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1 [7].
• A histone variant, Htz1p, and a Sir1p-like protein, Esc2p, mediate silencing at HMR [11].
• Additionally, the spectra of phenotypes, genes, and telomeres affected in yaf9Delta and htz1Delta strains are significantly similar, further supporting a role for Yaf9 in Htz1 deposition [12].

References