The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

umuD  -  translesion error-prone DNA polymerase V...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1171, JW1172
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of umuD

  • Remarkably, the umuC122::Tn5 allele of umuC, dinB, and certain forms of umuD gene products endow E. coli with the ability to withstand HU treatment (HUR) [1].
  • The umuD and umuC genes were placed under the control of a regulated phage lambda PL promoter and a synthetic ribosome-binding site, and the distance to the UmuD start was adjusted to maximize gene expression [2].
  • The left arm contains a long cluster of structural genes that are closely related to those of the lambda-like phages, but also includes homologs of umuD', which encodes a DNA polymerase accessory protein, and the plasmid partition genes, sopA and sopB [3].
  • The DNA from none of the vibrio species examined including marine vibrios hybridized to E. coli umuC and umuD gene sequences [4].
 

High impact information on umuD

  • Posttranslational modification of the umuD-encoded subunit of Escherichia coli DNA polymerase V regulates its interactions with the beta processivity clamp [5].
  • To initiate a biochemical characterization of the role of these proteins, we have developed a plasmid system that gives efficient expression of the umuD and umuC genes [2].
  • Furthermore, the search identified an SOS gene within the genome of P1 which encodes a protein that is homologous to UmuD', the RecA-promoted cleavage product of the umuD gene [6].
  • The umuD open reading frame is intact, but it is 1.5 times the size of other umuD genes and has an extra 5' region that lacks homology to known umuD genes [7].
  • The umuD mutation did not affect the growth rate or survival after UV-induced DNA damage [7].
 

Biological context of umuD

 

Associations of umuD with chemical compounds

 

Other interactions of umuD

  • From these and the previous results of genetic and biochemical studies on the cloned genes, we conclude that the former is the umuD and the latter is the umuC gene [14].
 

Analytical, diagnostic and therapeutic context of umuD

References

  1. Y-family DNA polymerases respond to DNA damage-independent inhibition of replication fork progression. Godoy, V.G., Jarosz, D.F., Walker, F.L., Simmons, L.A., Walker, G.C. EMBO J. (2006) [Pubmed]
  2. UmuD mutagenesis protein of Escherichia coli: overproduction, purification, and cleavage by RecA. Burckhardt, S.E., Woodgate, R., Scheuermann, R.H., Echols, H. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  3. Genomic sequence and analysis of the atypical temperate bacteriophage N15. Ravin, V., Ravin, N., Casjens, S., Ford, M.E., Hatfull, G.F., Hendrix, R.W. J. Mol. Biol. (2000) [Pubmed]
  4. Lack of umuDC gene functions in Vibrio cholerae cells. Ghosh, S.K., Panda, D.K., Das, J. Mutat. Res. (1989) [Pubmed]
  5. Posttranslational modification of the umuD-encoded subunit of Escherichia coli DNA polymerase V regulates its interactions with the beta processivity clamp. Sutton, M.D., Narumi, I., Walker, G.C. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  6. Identification of high affinity binding sites for LexA which define new DNA damage-inducible genes in Escherichia coli. Lewis, L.K., Harlow, G.R., Gregg-Jolly, L.A., Mount, D.W. J. Mol. Biol. (1994) [Pubmed]
  7. A constitutively expressed, truncated umuDC operon regulates the recA-dependent DNA damage induction of a gene in Acinetobacter baylyi strain ADP1. Hare, J.M., Perkins, S.N., Gregg-Jolly, L.A. Appl. Environ. Microbiol. (2006) [Pubmed]
  8. Mutation and killing of Escherichia coli expressing a cloned Bacillus subtilis gene whose product alters DNA conformation. Setlow, J.K., Randesi, M., Adams, J.G., Setlow, B., Setlow, P. J. Bacteriol. (1992) [Pubmed]
  9. Cloning and characterization of the umu operon responsible for inducible mutagenesis in Escherichia coli. Shinagawa, H., Kato, T., Ise, T., Makino, K., Nakata, A. Gene (1983) [Pubmed]
  10. Structural characterization of the Salmonella typhimurium LT2 umu operon. Thomas, S.M., Crowne, H.M., Pidsley, S.C., Sedgwick, S.G. J. Bacteriol. (1990) [Pubmed]
  11. Processing of MucA protein is required for spontaneous and benzo[a]pyrene-induced reversion of the Escherichia coli trpA23 missense mutation by G.C-T.A transversions: effect of a deficiency in the MutY DNA glycosylase. Urios, A., Herrera, G., Aleixandre, V., Blanco, M. Mutat. Res. (1994) [Pubmed]
  12. Induction of the SOS gene (umuC) by 4-quinolone antibacterial drugs. Power, E.G., Phillips, I. J. Med. Microbiol. (1992) [Pubmed]
  13. Novel Escherichia coli umuD' mutants: structure-function insights into SOS mutagenesis. McLenigan, M., Peat, T.S., Frank, E.G., McDonald, J.P., Gonzalez, M., Levine, A.S., Hendrickson, W.A., Woodgate, R. J. Bacteriol. (1998) [Pubmed]
  14. Structural analysis of the umu operon required for inducible mutagenesis in Escherichia coli. Kitagawa, Y., Akaboshi, E., Shinagawa, H., Horii, T., Ogawa, H., Kato, T. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  15. Identification of a umuDC locus in Salmonella typhimurium LT2. Smith, C.M., Eisenstadt, E. J. Bacteriol. (1989) [Pubmed]
 
WikiGenes - Universities