Disease relevance of Fast fuchsine G

• By the combination of tissue section staining with chromotrope 2R with in situ hybridization using the same TGF beta 1 probe, we found that approximately 50% of the eosinophils infiltrating the polyp tissue express the TGF beta 1 gene [1].
• Tissue diagnosis of intestinal microsporidiosis using the chromotrope-2R modified trichrome stain [2].
• The histological features of case 1 resembled membranous glomerulonephritis (MGN) on hematoxylin-eosin stain, but revealed DDD on periodic acid Schiff and chromotrope-2R silver methenamine stains [3].

High impact information on Fast fuchsine G

• In situ hybridization combined with histochemical staining with chromotrope 2R indicate that most of the cells expressing MCP-1 mRNA at these time points are primarily eosinophils [4].
• Counterstaining with chromotrope 2R demonstrated that virtually all cells expressing TNF-alpha message were eosinophils [5].
• Eo/B colonies, especially carbol chromotrope 2R+ cells, selected at Days 10, 14, and 18 from atopic donors contained messenger RNA for GM-CSF by combined in situ reverse transcription-polymerase chain reaction and cytochemistry, and demonstrated time-dependent expression of GM-CSF by immunocytochemistry (P = 0.007) [6].
• Furthermore, RANTES mRNA localized to carbol chromotrope 2R-positive colony cells, as assessed using in situ RT-PCR on day 21 of culture in semisolid media, and was found to gradually decrease (relative to beta2-microglobulin) in rhIL-3- and rhIL-5-treated colony cells (comprising > 90% eosinophil-like cells) up to day 28 [7].
• Approximately 90% of the TGF-beta 2 positive cells detected in the sections taken at day 78 also stained with chromotrope 2R used to detect eosinophils and the morphology of approximately 50% of these cells was characteristic of eosinophils.(ABSTRACT TRUNCATED AT 250 WORDS)[8]

Biological context of Fast fuchsine G

• The diagnosis was made on cytologic examination of the material processed according to the Papanicolaou technique, evaluated by Giemsa, Gram's, Grocott and chromotrope-2R modified trichrome preparations and by fungi-fluor fluorescent mycology stain [9].
• CONCLUSION: Detection of microsporidia in bile can be achieved using several different stains routinely available to cytologists, most optimally with alcohol-fixed Papanicolaou- or Giemsa-stained preparations or with Chromotrope 2R stain, which is available in parasitology laboratories [10].

Anatomical context of Fast fuchsine G

• Accumulation of chromotrope 2R positive cells in equine endometrium during early pregnancy and expression of transforming growth factor-beta 2 (TGF-beta 2) [8].
• A survey utilizing chromotrope 2R stain and fluorescent in situ hybridization techniques for testing feces from 193 captive mammals demonstrated that 3 animals (1.6%) shed Encephalitozoon bieneusi spores [11].

Associations of Fast fuchsine G with other chemical compounds

• Colocalization studies with chromotrope 2R revealed that only 4% of FcepsilonRIalpha+ cells were eosinophils after control challenge and, in contrast, 85 to 95% of FcepsilonRIalpha+ cells were eosinophils after allergen [12].
• Respondents answered comprehensive questionnaires covering clinical and epidemiologic information and provided stool specimens monthly, which were examined unstained as well as stained with trichrome, chromotrope 2R, and with Kinyoun carbol-fuchsin, and with indirect immunofluorescence for Cryptosporidium [13].

Gene context of Fast fuchsine G

• The RRS methods have high sensitivity for the determination of protein, the detection limits for bovine serum albumin (BSA) are 10.0 - 30.2 ng/ml, and the sensitivity order is CALKS > AAI = CPAI > ACBK = CT2R > ACDB > CBSE [14].

Analytical, diagnostic and therapeutic context of Fast fuchsine G

• The IFAT was validated against standard staining methods (Chromotrope 2R and Uvitex 2B) and PCR [15].
• Urine and stool specimens were analyzed by modified chromotrope 2R trichrome and chitin fluorochrome stains and by transmission electron microscopy [16].

References