The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

tsf  -  elongation factor Ts

Escherichia coli CFT073

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of tsf


High impact information on tsf


Biological context of tsf


Anatomical context of tsf


Associations of tsf with chemical compounds

  • The role of cysteinyl residues in the activity of bacterial elongation factor Ts, a guanosine nucleotide dissociation protein [14].
  • Replacement of Cys-22 by Ser or Gly only partially impairs the binding or catalytic properties of EF-Ts while it completely protects EF-Ts from the inactivation by NEM [14].
  • The present results also indicate that the deletion alters the competition between EF-Ts and kirromycin for the binding to EF-Tu [12].

Regulatory relationships of tsf

  • The effects are attributed to the interference of the mutations with the EF-Ts-induced movements of the P-loop of EF-Tu and changes at the domain 1/3 interface, leading to the release of the beta-phosphate group of GTP/GDP [15].

Other interactions of tsf

  • The kinetic results suggest that the initial contact of EF-Ts with helix D of EF-Tu weakens binding interactions around the guanine base, whereas contacts of EF-Ts with the phosphate binding side that promotes the release of the phosphate moiety of GDP appear to take place later [16].

Analytical, diagnostic and therapeutic context of tsf


  1. Elongation factor Ts of Chlamydia trachomatis: structure of the gene and properties of the protein. Zhang, Y., Tao, J., Zhou, M., Meng, Q., Zhang, L., Shen, L., Klein, R., Miller, D.L. Arch. Biochem. Biophys. (1997) [Pubmed]
  2. Qbeta-phage resistance by deletion of the coiled-coil motif in elongation factor Ts. Karring, H., Mathu, S.G., van Duin, J., Clark, B.F., Kraal, B., Knudsen, C.R. J. Biol. Chem. (2004) [Pubmed]
  3. Structure and importance of the dimerization domain in elongation factor Ts from Thermus thermophilus. Jiang, Y., Nock, S., Nesper, M., Sprinzl, M., Sigler, P.B. Biochemistry (1996) [Pubmed]
  4. Cloning and partial characterization of the gene encoding the putative elongation factor Ts of Streptococcus suis serotype 2. Martinez, G., Harel, J., Lacouture, S., Gottschalk, M. Arch. Microbiol. (2003) [Pubmed]
  5. Inhibition of RNA polymerase activity by the Escherichia coli protein biosynthesis elongation factor Ts. Biebricher, C.K., Druminski, M. Proc. Natl. Acad. Sci. U.S.A. (1980) [Pubmed]
  6. mRNA-dependent in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu. Chu, F., Caldwell, P., Weissbach, H., Brot, N. Proc. Natl. Acad. Sci. U.S.A. (1977) [Pubmed]
  7. Mutagenesis of bacterial elongation factor Tu at lysine 136. A conserved amino acid in GTP regulatory proteins. Hwang, Y.W., Sanchez, A., Miller, D.L. J. Biol. Chem. (1989) [Pubmed]
  8. A kinetic analysis of the interaction of elongation factor Tu with guanosine nucleotides and elongation factor Ts. Eccleston, J.F. J. Biol. Chem. (1984) [Pubmed]
  9. Inactivation of Escherichia coli elongation factor Ts by the arginine-specific reagent butanedione. MarSchel, A.H., Bodley, J.W. J. Biol. Chem. (1979) [Pubmed]
  10. Large ATP synthase operon of the red alga Antithamnion sp. resembles the corresponding operon in cyanobacteria. Kostrzewa, M., Zetsche, K. J. Mol. Biol. (1992) [Pubmed]
  11. Kinetic mechanism of elongation factor Ts-catalyzed nucleotide exchange in elongation factor Tu. Gromadski, K.B., Wieden, H.J., Rodnina, M.V. Biochemistry (2002) [Pubmed]
  12. Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts. Karring, H., Björnsson, A., Thirup, S., Clark, B.F., Knudsen, C.R. Eur. J. Biochem. (2003) [Pubmed]
  13. cDNA sequence of a translational elongation factor Ts homologue from Caenorhabditis elegans: mitochondrial factor-specific features found in the nematode homologue peptide. Watanabe, Y., Kita, K., Ueda, T., Watanabe, K. Biochim. Biophys. Acta (1997) [Pubmed]
  14. The role of cysteinyl residues in the activity of bacterial elongation factor Ts, a guanosine nucleotide dissociation protein. Hwang, Y.W., Sanchez, A., Hwang, M.C., Miller, D.L. Arch. Biochem. Biophys. (1997) [Pubmed]
  15. The importance of P-loop and domain movements in EF-Tu for guanine nucleotide exchange. Dahl, L.D., Wieden, H.J., Rodnina, M.V., Knudsen, C.R. J. Biol. Chem. (2006) [Pubmed]
  16. Mechanism of elongation factor (EF)-Ts-catalyzed nucleotide exchange in EF-Tu. Contribution of contacts at the guanine base. Wieden, H.J., Gromadski, K., Rodnin, D., Rodnina, M.V. J. Biol. Chem. (2002) [Pubmed]
WikiGenes - Universities