Disease relevance of Nhcp1

• This protein, which comigrates with a nonhistone chromosomal protein present in the human cell line used to produce this hybrid cell and which is also present in two additional human cells lines studied, could not be detected in the mouse erythroleukemia parent before fusion [1].
• A functional relationship between changes in the nonhistone chromosomal proteins and variations in the transcriptional activity accompanying differentiation of neuroblastoma cells may exist [2].
• Metabolic stability of nonhistone chromosomal proteins in Ehrlich ascites tumour cells [3].

High impact information on Nhcp1

• The class of nonhistone chromosomal proteins that remains bound to DNA in chromatin in the presence of 2.5 M NaCl-5 M urea has proven refractile to biochemical analysis [4].
• The results verify the hypothesis that a specific nonhistone chromosomal protein is preferentially associated with the set of loci that includes both active sites and those scheduled to be active at some time in this developmental program [5].
• The nonhistone chromosomal proteins of a series of hybrid mouse erythroleukemia cell lines containing human chromosome 16 were investigated by two-dimensional gel electrophoresis to determine if such cells contained nonhistone chromosomal proteins of both human and mouse origin [1].
• In contrast, there were significant differences in the nonhistone chromosomal proteins synthesized and associated with the genome of proliferating and differentiating neuroblastoma cells [2].
• The high-mobility group I (HMGI) nonhistone chromosomal proteins HMGI(Y) and HMGI-C have been implicated in defining chromatin structure and in regulating the transcription of several genes [6].

Biological context of Nhcp1

• The two-dimensional gel electrophoretogram of a cloned hybrid mouse erythroleukemia cell line that retained a tetraploid complement of mouse chromosomes and human chromosome 16 (as the only human chromosome) displayed a nonhistone chromosomal protein of pI 6.2 and Mr 65,000 [1].
• A search of a data base revealed that the T160 protein has significant sequence homology (56%) to the nonhistone chromosomal protein HMG1 within the C-terminal region of 80 amino acids [7].
• The high mobility group (HMG) proteins I and Y are well characterized nonhistone chromosomal proteins which bind to A.T-rich regions of DNA, and may regulate gene expression and/or DNA replication [8].
• Nucleotide sequence of a mouse cDNA encoding the nonhistone chromosomal high mobility group protein-1 (HMG1) [9].
• Elevated levels of high mobility group (HMG) nonhistone chromosomal proteins I and Y, alternatively spliced members of the HMG-I(Y) family of architectural transcription factors, have been linked with human cancer and with neo-plastic and metastatic phenotypes in model systems [10].

Anatomical context of Nhcp1

• Comparison of the two-dimensional gel electrophoretograms of the nonhistone chromosomal proteins of mouse and human cell lines showed 400 and 280 chromosomal proteins, respectively, of which about 75% were electrophoretically identical [1].

Associations of Nhcp1 with chemical compounds

• Inhibition of the phosphorylation of nonhistone chromosomal proteins by cordycepin and xylosyladenine in L1210 cells in vitro [11].
• A nonhistone chromosomal protein of 54,000 mol wt (T4) was found in chromatin preparations from androgen-stimulated A/J mice [12].
• This soluble (S) fraction was relatively depleted in H1 histones and methylated DNA (5-methylcytosine) but highly enriched in RNA, single-stranded DNA, and nonhistone chromosomal proteins, particularly two species of the high mobility group identified as HMG 1 and HMG 2 [13].
• Fractionation of nonhistone chromosomal proteins utilizing hydroxyapatite chromatography [14].

Physical interactions of Nhcp1

• Identification of nonhistone chromosomal proteins binding specifically to cloned alpha-fetoprotein DNA sequences [15].

Other interactions of Nhcp1

• HMG-17 is an abundant, nonhistone chromosomal protein that binds preferentially to nucleosomal core particles of mammalian chromatin [16].
• Nuclear estrogen receptor and nonhistone chromosomal proteins in hormonal independency of murine breast cancers [17].

Analytical, diagnostic and therapeutic context of Nhcp1

• Of the 400+ NHCP spots detected in RI 2D-PAGE maps, 172 were common to progenitors and all RIs [18].
• Induction of early transcription in one-cell mouse embryos by microinjection of the nonhistone chromosomal protein HMG-I [19].
• We have examined, by protein binding assays, thermal denaturation, and circular dichroism, the possible effects of histones on nonhistone chromosomal protein (NHCP) interactions with DNA [20].
• Fractionation of nonhistone chromosomal proteins utilizing isoelectric focusing techniques [21].

References