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Gene Review

ros  -  transcriptional regulator

Agrobacterium fabrum str. C58

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Disease relevance of ros

  • Site-directed mutagenesis of Cys-82 and His-92 in this motif showed that these residues are essential for Zn2+ and DNA binding activities of Ros. The existence of such a regulator in Agrobacterium may be due to horizontal interkingdom retrotransfer of the ros gene from plant to bacteria [1].
  • The ros gene is highly conserved in members of the Rhizobiaceae [2].

High impact information on ros

  • An ipt promoter::cat reporter gene fusion showed a 10-fold increase in ipt promoter activity in A. tumefaciens ros mutant strains when compared with wild type [1].
  • Also, increased levels (10- to 20-fold) of isopentenyl adenosine, the product of the reaction catalyzed by isopentenyl transferase, were detected in ros mutant strains [1].
  • This mutant affected the expression of exoY but not of ros [3].
  • It was shown that the two cysteine residues in the Ros protein were essential for the complementation of the exopolysaccharide synthesis defect of ros mutant strains [3].
  • Pleiotropic effects of regulatory ros mutants of Agrobacterium radiobacter and their interaction with Fe and glucose [4].

Chemical compound and disease context of ros


Biological context of ros

  • The ros mutation is chromosomal in nature and is characterized by a more-than-300-fold increase in the level of expression of bak and a 12-fold increase in the level of expression of an adjacent divergent operon containing the hdv genes, which are involved in some aspect of host specificity [7].
  • Genetic and biochemical evidence indicates that the product of the ros locus is a negative regulator of Ti plasmid genes and is related to undefined chromosomally encoded functions that are involved in the mutant phenotype [7].
  • Reporter fusion to the ros gene indicates that the level of transcription is controlled in part by autoregulation [5].
  • Mapping of the ros virulence regulatory gene of A. tumefaciens [8].
  • Interestingly, the mutation in 4011R is an Arg to Cys conversion at amino acid residue 125 near the C-terminus well outside the zinc finger of Ros. Yet, Ros bearing this mutation is unable to bind to the Ros-box and is unable to complement other ros mutants [9].

Associations of ros with chemical compounds

  • The effects of the ros mutation and acetosyringone were cumulative for all vir promoters examined [6].
  • The pleiotropic characteristics of the ros mutant include the complete absence of the major acidic capsular polysaccharide [6].

Regulatory relationships of ros


Analytical, diagnostic and therapeutic context of ros


  1. Agrobacterium transcriptional regulator Ros is a prokaryotic zinc finger protein that regulates the plant oncogene ipt. Chou, A.Y., Archdeacon, J., Kado, C.I. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  2. Negative transcriptional regulation of virulence and oncogenes of the Ti plasmid by Ros bearing a conserved C2H2-zinc finger motif. Kado, C.I. Plasmid (2002) [Pubmed]
  3. Iron-dependent transcription of the regulatory gene ros of Agrobacterium radiobacter. Hussain, H., Johnston, A.W. Mol. Plant Microbe Interact. (1997) [Pubmed]
  4. Pleiotropic effects of regulatory ros mutants of Agrobacterium radiobacter and their interaction with Fe and glucose. Brightwell, G., Hussain, H., Tiburtius, A., Yeoman, K.H., Johnston, A.W. Mol. Plant Microbe Interact. (1995) [Pubmed]
  5. The virC and virD operons of the Agrobacterium Ti plasmid are regulated by the ros chromosomal gene: analysis of the cloned ros gene. Cooley, M.B., D'Souza, M.R., Kado, C.I. J. Bacteriol. (1991) [Pubmed]
  6. Dual control of Agrobacterium tumefaciens Ti plasmid virulence genes. Close, T.J., Rogowsky, P.M., Kado, C.I., Winans, S.C., Yanofsky, M.F., Nester, E.W. J. Bacteriol. (1987) [Pubmed]
  7. Regulation of Ti plasmid virulence genes by a chromosomal locus of Agrobacterium tumefaciens. Close, T.J., Tait, R.C., Kado, C.I. J. Bacteriol. (1985) [Pubmed]
  8. Mapping of the ros virulence regulatory gene of A. tumefaciens. Cooley, M.B., Kado, C.I. Mol. Gen. Genet. (1991) [Pubmed]
  9. A single amino acid substitution beyond the C2H2-zinc finger in Ros derepresses virulence and T-DNA genes in Agrobacterium tumefaciens. Archdeacon, J., Bouhouche, N., O'Connell, F., Kado, C.I. FEMS Microbiol. Lett. (2000) [Pubmed]
  10. Molecular characterization of the virC genes of the Ti plasmid. Close, T.J., Tait, R.C., Rempel, H.C., Hirooka, T., Kim, L., Kado, C.I. J. Bacteriol. (1987) [Pubmed]
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