High impact information on Dnm2

• We report here that dynamin 2, a ubiquitously expressed dynamin isoform, has a role in phagocytosis in macrophages [1].
• Dynamin 2 is required for phagocytosis in macrophages [1].
• Dynamin 2 is enriched on early phagosomes, and expression of a dominant-negative mutant of dynamin 2 significantly inhibits particle internalization at the stage of membrane extension around the particle [1].
• These studies show that Arc/Arg3.1 regulates endophilin 3 and dynamin 2, two components of the endocytosis machinery [2].
• We now report that this internalization is mediated by dynamin-2 and caveolin-1 [3].

Biological context of Dnm2

• Taken together, these results suggest that etorphine-induced down-regulation may depend upon mu-opioid receptor degradation and changes in dynamin-2-mediated receptor trafficking [4].
• Antagonist-induced micro-opioid receptor up-regulation decreases G-protein receptor kinase-2 and dynamin-2 abundance in mouse spinal cord [5].
• Its co-localization and interaction with complexin I suggest that dynamin 2 may play a role during acrosome formation and/or acrosomal exocytosis [6].
• Mu-opioid receptor up-regulation has been associated with decreases in the trafficking protein dynamin-2, whereas mu-opioid receptor down-regulation produces an increase in dynamin-2 abundance [4].

Anatomical context of Dnm2

• To define how Dyn2 might participate in actin dynamics in livings cells we have expressed green fluorescent protein (GFP)-tagged Dyn2 in cultured cells and observed labeling of comet-like vesicles and macropinosomes [7].
• To determine the role of caveolae internalization in the mechanism of NO production, we expressed dominant-negative dynamin-2 mutant (K44A) or treated cells with methyl-beta-cyclodextrin [8].
• Therefore, we examined etorphine and morphine effects on regulation of GRK-2 and DYN-2 in mouse spinal cord [9].
• Dynamin 2 protein was detected in mouse testis and was most abundant in or around the developing acrosome of spermatids [6].

Associations of Dnm2 with chemical compounds

• Delivery of the same daily dose of etorphine or naloxone using intermittent (every 24 h for 7 days) s.c. administration had no effect on immunoreactive mu-opioid receptor, [3H] DAMGO binding or dynamin-2 abundance [4].

Other interactions of Dnm2

• Thus, dynamin 2 may be the constitutive counterpart of dynamin 1 in growing neurons [10].
• The large GTPase dynamin (Dyn2) has been demonstrated by us and others to interact with several different actin-binding proteins [7].
• Etorphine increased ( approximately 70%) DYN-2 protein abundance and decreased its mRNA (31%), whereas it had no effect on GRK-2 protein and mRNA abundance [9].

References