Disease relevance of Slc26a3

• Mutations in the SLC26A3 (DRA (down-regulated in adenoma)) gene constitute the molecular etiology of congenital chloride-losing diarrhea in humans [1].

High impact information on Slc26a3

• The plasma membrane expression of major apical anion exchanger DRA (SLC26A3) was considerably reduced in EPEC-infected cells, corresponding with decreased Cl(-)/OH(-) exchange activity [2].
• Our data demonstrate that EspG and EspG2 play an important role in contributing to EPEC infection-associated inhibition of luminal membrane chloride transport via modulation of surface DRA expression [2].
• The Dra mutation has previously been shown to abrogate phosphorylation as well as T-Ag binding [3].
• Using an RNase protection assay, we found that dra mRNA expression is limited to the small intestine and colon in mouse, therefore identifying Dra as an intestine-specific sulfate transporter. dra also had a unique pattern of expression during intestinal development [4].
• This functional characterization provides the foundation for investigation of the role of Dra in intestinal sulfate transport and in the malignant phenotype [4].

Biological context of Slc26a3

• In conclusion, these results point to the role of Slc26a6 in HCO(3)(-) efflux at the apical membrane and also suggest the presence of a robust Slc26a3 compensatory upregulation, which can replace the function of Slc26a6 in pancreatic ducts [5].
• The down-regulated in adenoma (Dra) gene encoding intestine-specific anion transporter maps to mouse chromosome 12 [6].
• We have established a strain of transgenic mice in which the HLA-DRA gene was integrated into the X-chromosome and the xenogeneic mixed isotype molecule, DR alpha E beta b, was expressed in a cell type-specific manner, although the transgenic DRA gene contained only 268 base pairs of the 5'-flanking region [7].
• SSCP analysis of Dra I-digested PCR product of 393 bp also showed different patterns in FD2 and FD7 samples [8].

Associations of Slc26a3 with chemical compounds

• To investigate the evolutionary dynamics at Mhc class II DR genes of mice (genus Mus), we sequenced the peptide binding regions (PBRs) of 41 DRB (= E beta) genes and eight DRA (= E alpha) genes from 15 strains representing eight species [9].
• Tca and Esa are assigned provisionally to the first short-consensus repeat (SCR), UMC to the second SCR, Dra to the third SCR and Cra, WESa and WESb to the fourth SCR or to the serine/threonine rich region of the DAF protein [10].

Regulatory relationships of Slc26a3

• The mRNA expression of Slc26a3 (downregulated in adenoma) and Slc26a6 (putative anion exchanger-1) was similar between WT and CF duodena [11].

Analytical, diagnostic and therapeutic context of Slc26a3

• Fluorescence-activated cell sorting (FACS) analysis revealed that cultures induced to overexpress either wild-type or Dra mutant Rb proteins were significantly enriched for cells with a G2 DNA content [3].
• PCR of merozoite DNA using primer pairs JNB33/JNB54 and restriction enzyme digestion of the 1100 bp product with Dra I indicated the organism was S. neurona [12].

References