Disease relevance of UL21

• The BHV-1 ORFs were arranged colinearly with the prototype sequence of herpes simplex virus 1 in the range of the UL21 to UL4 genes [1].
• Virus-induced proteins in pseudorabies-infected cells. II. Proteins of the virion and nucleocapsid [2].
• The data support a model where virion subassemblies but not complete virions are transported in the axon [3].
• Our results provide new insight into the process of virion assembly and exit from neurons that leads to directional spread of herpesviruses in the nervous system [3].
• Identification of the bovine herpesvirus 1 circ protein, a myristylated and virion-associated polypeptide which is not essential for virus replication in cell culture [4].

High impact information on UL21

• Our data suggest that tyrosine phosphorylation of VP22 plays a role in virion assembly [5].
• Bovine herpesvirus 1 glycoprotein B does not productively interact with cell surface heparan sulfate in a pseudorabies virion background [6].
• In herpes simplex virus (HSV)-infected cells, the transcription of immediate-early (alpha) genes is regulated by a virion component, the alpha gene trans-inducing factor (alpha TIF) [7].
• An electrophoretic analysis of radioactively labeled, purified, "empty" and DNA-containing infectious bovine rhinotracheitis virions revealed the presence of 25 to 33 structural (virion) polypeptides [8].
• However, concatemeric termini exhibited a preponderance of L termini derived from P isomers which was comparable to the preponderance of P genomes found in virion DNA [9].

Biological context of UL21

• Nucleotide sequence analysis of a 30-kb region of the bovine herpesvirus 1 genome which exhibits a colinear gene arrangement with the UL21 to UL4 genes of herpes simplex virus [1].
• Phosphorylation of at least one virion protein is shown to take place in vivo [2].
• Similar to the HSV vhs activity, the putative BHV-1 vhs activity was not abrogated in cells infected in the presence of actinomycin D (ActD) which suggested that no viral gene expression is required for the vhs function and the putative vhs protein is associated with the virion [10].

Anatomical context of UL21

• During virus replication a fraction of the intranuclear DNA of bovine herpesvirus-1 (BHV-1) was present in the nucleosomal structure of infected eukaryotic cells, and virion proteins were associated with the chromatin of virus infected cells [11].

Associations of UL21 with chemical compounds

• Bovine herpesvirus type 1 (BHV-1) glycoprotein H (gH) is a structural component of the virion which forms a complex with glycoprotein gL [12].

Analytical, diagnostic and therapeutic context of UL21

• Using a rabbit antiserum raised against a synthetic oligopeptide representing the carboxy terminus of the predicted circ polypeptide for Western blot (immunoblot) analyses and immunofluorescence assays, we identified a 34-kDa virion-associated protein which accumulated in the cytoplasm of infected cells [4].
• This fixative stabilized nucleic acids, allowing acetone dehydration and plastic embedding without collapse of nucleic acid to the centre of the virion [13].
• Proteins and glycoproteins of virion of VZV were analyzed by immunoprecipitation with antisera against partially purified virion of VZV [14].

References