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Gene Review

CYP2F1  -  cytochrome P450, family 2, subfamily F,...

Homo sapiens

Synonyms: C2F1, CYP2F, CYPIIF1, Cytochrome P450 2F1
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Disease relevance of CYP2F1

  • To better understand the mechanism for the unique toxicity profiles for both compounds, a CYP2F ortholog (CYP2F4) was isolated from rat lung and expressed using a baculovirus system [1].
  • Styrene respiratory tract toxicity and mouse lung tumors are mediated by CYP2F-generated metabolites [2].

High impact information on CYP2F1


Biological context of CYP2F1


Anatomical context of CYP2F1

  • CYP2F1, 4B1, 4F8, 11s (11A, 11B1, and 11B2), 19, and 24 mRNA levels were the highest in the lung, lung, prostate, adrenal gland, placenta, and kidney, respectively; however, the mRNA expression of these eight CYP isoforms in the liver was not detected by this method [9].
  • Benzene metabolism in human lung cell lines BEAS-2B and A549 and cells overexpressing CYP2F1 [10].
  • We have developed a method using liquid chromatography/mass spectrometry (LC/MS) to evaluate the kinetics of the rates of production of conjugate [C] by recombinant CYP2E1 and CYP2F enzymes and lung microsomes [11].
  • Immunisation with one of C1, C2F1, C2F2, or C2F3 caused milder arthritis, even though each of the fragments carried T cell epitopes [12].

Associations of CYP2F1 with chemical compounds

  • Remarkable product preferences between the desaturation pathway to form the methyleneindolenine by CYP2F1 and the ring epoxidation pathway to form the oxindole by CYP1A2, were observed [13].
  • Human CYP2F1 bioactivates 3-methylindole (3MI), while mouse CYP2F2 bioactivates naphthalene [7].
  • The enzyme, however, lacked catalytic activity with other common P450 substrates including 7-ethoxycoumarin, a substrate for CYP2F1, indicating that the substrate selectivity of CYP2F3 appears to be high [7].
  • The use of the CYP2E1-selective inhibitor diethyldithiocarbamate and the CYP2F2-selective inhibitor 5-phenyl-1-pentyne demonstrated that both CYP2E1 and CYP2F1 are important in benzene metabolism in the BEAS-2B and A549 human lung cell lines [10].
  • Bioactivation of naphthalene to its pneumotoxic epoxide by CYP2F1 was also demonstrated [14].

Other interactions of CYP2F1

  • CYP1A1 levels were approximately an order of magnitude lower (3.9 x 10(4) molecules/microg total RNA), followed by CYP2F1 and CYP3A levels that were near the detection limit of the assay [15].
  • Using RT-PCR analysis, basal expression of mRNAs coding for CYP2B7, CYP2F1 and CYP2E1 were detected in cultures from several donors [16].
  • Compared to other divisions of the CYP2 gene family, the CYP2F subfamily is unusual in having few genes per species and in being selectively expressed in lung tissues [17].

Analytical, diagnostic and therapeutic context of CYP2F1


  1. Bioactivation of the pulmonary toxicants naphthalene and 1-nitronaphthalene by rat CYP2F4. Baldwin, R.M., Shultz, M.A., Buckpitt, A.R. J. Pharmacol. Exp. Ther. (2005) [Pubmed]
  2. Styrene respiratory tract toxicity and mouse lung tumors are mediated by CYP2F-generated metabolites. Cruzan, G., Carlson, G.P., Johnson, K.A., Andrews, L.S., Banton, M.I., Bevan, C., Cushman, J.R. Regulatory toxicology and pharmacology : RTP. (2002) [Pubmed]
  3. Metabolic activation of 4-ipomeanol by complementary DNA-expressed human cytochromes P-450: evidence for species-specific metabolism. Czerwinski, M., McLemore, T.L., Philpot, R.M., Nhamburo, P.T., Korzekwa, K., Gelboin, H.V., Gonzalez, F.J. Cancer Res. (1991) [Pubmed]
  4. Characterization of the human lung CYP2F1 gene and identification of a novel lung-specific binding motif. Carr, B.A., Wan, J., Hines, R.N., Yost, G.S. J. Biol. Chem. (2003) [Pubmed]
  5. Localization of CYP2F1 by multipoint linkage analysis and pulsed-field gel electrophoresis. Bale, A.E., Mitchell, A.L., Gonzalez, F.J., McBride, O.W. Genomics (1991) [Pubmed]
  6. Selective dehydrogenation/oxygenation of 3-methylindole by cytochrome p450 enzymes. Lanza, D.L., Yost, G.S. Drug Metab. Dispos. (2001) [Pubmed]
  7. Cloning and expression of CYP2F3, a cytochrome P450 that bioactivates the selective pneumotoxins 3-methylindole and naphthalene. Wang, H., Lanza, D.L., Yost, G.S. Arch. Biochem. Biophys. (1998) [Pubmed]
  8. 3-methylindole-induced toxicity to human bronchial epithelial cell lines. Nichols, W.K., Mehta, R., Skordos, K., Macé, K., Pfeifer, A.M., Carr, B.A., Minko, T., Burchiel, S.W., Yost, G.S. Toxicol. Sci. (2003) [Pubmed]
  9. Tissue distribution of mRNA expression of human cytochrome P450 isoforms assessed by high-sensitivity real-time reverse transcription PCR. Nishimura, M., Yaguti, H., Yoshitsugu, H., Naito, S., Satoh, T. Yakugaku Zasshi (2003) [Pubmed]
  10. Benzene metabolism in human lung cell lines BEAS-2B and A549 and cells overexpressing CYP2F1. Sheets, P.L., Yost, G.S., Carlson, G.P. J. Biochem. Mol. Toxicol. (2004) [Pubmed]
  11. Bioactivation of 1,1-dichloroethylene to its epoxide by CYP2E1 and CYP2F enzymes. Simmonds, A.C., Reilly, C.A., Baldwin, R.M., Ghanayem, B.I., Lanza, D.L., Yost, G.S., Collins, K.S., Forkert, P.G. Drug Metab. Dispos. (2004) [Pubmed]
  12. Characterisation of cartilage intermediate layer protein (CILP)-induced arthropathy in mice. Yao, Z., Nakamura, H., Masuko-Hongo, K., Suzuki-Kurokawa, M., Nishioka, K., Kato, T. Ann. Rheum. Dis. (2004) [Pubmed]
  13. Metabolism of 3-methylindole by vaccinia-expressed P450 enzymes: correlation of 3-methyleneindolenine formation and protein-binding. Thornton-Manning, J., Appleton, M.L., Gonzalez, F.J., Yost, G.S. J. Pharmacol. Exp. Ther. (1996) [Pubmed]
  14. Specific dehydrogenation of 3-methylindole and epoxidation of naphthalene by recombinant human CYP2F1 expressed in lymphoblastoid cells. Lanza, D.L., Code, E., Crespi, C.L., Gonzalez, F.J., Yost, G.S. Drug Metab. Dispos. (1999) [Pubmed]
  15. Fingerprinting of cytochrome P450 and microsomal epoxide hydrolase gene expression in human blood cells. Krovat, B.C., Tracy, J.H., Omiecinski, C.J. Toxicol. Sci. (2000) [Pubmed]
  16. Expression of cytochrome P450 2E1 in normal human bronchial epithelial cells and activation by ethanol in culture. Runge, D.M., Stock, T.W., Lehmann, T., Taege, C., Bernauer, U., Stolz, D.B., Hofmann, S., Foth, H. Arch. Toxicol. (2001) [Pubmed]
  17. Identification and cross-species comparisons of CYP2F subfamily genes in mammals. Chen, N., Whitehead, S.E., Caillat, A.W., Gavit, K., Isphording, D.R., Kovacevic, D., McCreary, M.B., Hoffman, S.M. Mutat. Res. (2002) [Pubmed]
  18. The human CYP2F gene subfamily: identification of a cDNA encoding a new cytochrome P450, cDNA-directed expression, and chromosome mapping. Nhamburo, P.T., Kimura, S., McBride, O.W., Kozak, C.A., Gelboin, H.V., Gonzalez, F.J. Biochemistry (1990) [Pubmed]
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