Disease relevance of Slc40a1

• Interleukin-1beta up-regulates iron efflux in rat C6 glioma cells through modulation of ceruloplasmin and ferroportin-1 synthesis [1].

High impact information on Slc40a1

• Kupffer cells, which process senescent red blood cells and recycle the iron, had high levels of ferroportin 1, ceruloplasmin, and hephaestin mRNA [2].
• To investigate this possibility, the effects of L-DOPA on the expression of iron influx proteins [transferrin receptor (TfR) and divalent metal transporter 1 (DMT1)], iron efflux protein (ferroportin 1), and iron uptake in C6 glioma cells were determined in this study using Northern blot and Western blot analysis and the calcein method [3].
• Whether intestinal iron transporters, divalent metal transporter 1 (DMT1) and ferroportin 1 (FPN1), are present in the mammary gland and are involved in iron transfer into milk are unknown [4].
• x-Irradiation in Rat Liver: Consequent Upregulation of Hepcidin and Downregulation of Hemojuvelin and Ferroportin-1 Gene Expression [5].
• Purpose: To prospectively analyze hepcidin, hemojuvelin, and ferroportin-1 expression after x-irradiation of rat liver and isolated rat hepatocytes [5].

Biological context of Slc40a1

• By using RT-PCR, we demonstrate that FP and CP gene expression is up-regulated by treatment with the pro-inflammatory cytokine IL-1beta in rat C6 cells, taken as a glial cellular model [1].
• Taken together, these data suggest that hepcidin mediates LPS-induced downregulation of intestinal FPT1 expression and that the hepcidin signaling pathway involves a PDTC-sensitive step [6].
• Ferroportin may function as a modulator of DMT1 activity and play a role in Fe(III) uptake, possibly by affecting the number or affinity of citrate binding sites [7].
• CONCLUSIONS: The currently described CAR1 and CAR2 isoforms are not expressed in rat uterine tissue; therefore, they do not appear to participate in parturition in the pregnant rat [8].
• Ferroportin in the postnatal rat brain: implications for axonal transport and neuronal export of iron [9].

Anatomical context of Slc40a1

• Ferroportin mRNA localised to enterocytes of the villus only [10].
• The finding of ferroportin in iron deficient goblet cells also suggests an additional role to iron excretion [10].
• Ferroportin gene and protein expression in rat duodenum was studied by in situ hybridisation and immunohistochemistry, respectively in rats with different efficiencies of iron absorption [10].
• In addition to expression on the basolateral membrane of enterocytes, ferroportin is also seen on the microvillus membrane [10].
• Augmented internalisation of ferroportin to late endosomes impairs iron uptake by enterocyte-like IEC-6 cells [11].

Associations of Slc40a1 with chemical compounds

• In PDTC-treated rats, LPS stimulated no additional hepatic hepcidin expression but did increase intestinal FPT1 expression [6].
• Secondly, blocking ferroportin partly and totally reduced Fe(II) and Fe(III) uptake, respectively [7].
• Effects on plasma, milk, liver and mammary gland Fe and vitamin A concentrations, and divalent metal transporter-1 (DMT1), ferroportin (FPN), ferritin (Ft), and transferrin receptor (TfR) expression were determined [12].

Other interactions of Slc40a1

• Following stimulation with IL-1beta, a higher expression level of CP and FP was also confirmed by Western blotting [1].
• At mid- (PN10) and late (PN20) infancy, tissue Fe distribution, Fe absorption, intestine DMT1, ferroportin-1 (FPN) and hephaestin expression, and localization and liver hepcidin expression were measured [13].

Analytical, diagnostic and therapeutic context of Slc40a1

• Ferroportin and DMT1 distribution were assessed by immunofluorescence microscopy [11].

References