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Gene Review

Aagab  -  alpha- and gamma-adaptin binding protein

Rattus norvegicus

Synonyms: Alpha-and gamma-adaptin-binding protein p34, P34
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Disease relevance of P34

  • However, proliferative activation of baby rat kidney cells with adenovirus caused rapid induction of p34 synthesis [1].

High impact information on P34

  • At P40, following FB injections into the pyramidal decussation on P34, pyramidal tract neurons are labeled within the O----R transplants, but none can be labeled within R----O transplants, although in the same R----O cases transplanted neurons are labeled by an injection of DY in the superior colliculus [2].
  • In a second series of host rats, on P34 FB was injected in the pyramidal decussation of the O----R cases, or in the superior colliculus of the R----O cases, and in both groups DY was injected into the region of contralateral cortex homotopic for the new location of the transplant [2].
  • The major phosphoproteins pp80ac, pp46, and pp40 (Katz et al., 1985), as well as p34 partition into the oil phase of Triton X-114 extracts, suggesting that they are integral membrane proteins, at least in our experimental conditions [3].
  • These data are consistent with the possibility that p34 plays a role in cell division in higher vertebrates [1].
  • Here we have studied the distribution and synthesis of this 34 kd protein in rat tissues and cell lines. p34 was found in a variety of organs, including some such as brain that are not highly active in cell division [1].

Biological context of P34

  • METHODS: Postnatal day 20 (P20) rats were injected with lithium and pilocarpine to induce SE, and then with bromodeoxyuridine (BrdU) 4, 6, and 8 days later (P24, 26, and 28), and killed 1 day (P29), 1 week (P34), and 3 weeks (P50) after the last dose of BrdU for cell counts [4].
  • The cytoplasmic domain of p34 has several noteworthy structural features, including a region of 4.5 tandem repeats of 23-24 amino acids [5].
  • In the present study we isolated cDNA encoding p34 from a rat liver cDNA library and determined its complete amino acid sequence. p34 mRNA is 3.2 kb long and encodes a polypeptide of 307 amino acids with a molecular mass of about 34.9 kDa [5].
  • Here, antibodies directed against p34 were examined as to their inhibitory effect on ribosome binding to and protein translocation across the microsomal membrane [6].
  • We previously demonstrated that Mos translation in rat oocytes is negatively regulated by a PKA-mediated cAMP action, which inhibits c-mos mRNA polyadenylation and is associated with the suppression of p34 cdc2 kinase [7].

Anatomical context of P34

  • In sharp contrast, adipose tissue expression was highest between P3 and P5 but declined to a nadir at P20 before rebounding at P34 [8].
  • The p34 protein is a non-glycosylated, integral membrane protein characteristic of rough microsomes and is believed to play a role in the ribosome-membrane association [6].
  • These results suggest that p34 is involved in the binding of ribosomes to the microsomal membrane and that it is in close proximity to the protein translocation site in the microsomal membrane [6].
  • Immunochemical analyses using antibodies directed against p34 suggested that it is a membrane-embedded RM surface protein, which is specifically localized in ribosome-attached organelles and widely distributed among mammalian tissues [9].
  • The p38 and p34 polypeptides of growth cone particle membranes are the alpha- and beta-subunits of G proteins [10].

Associations of P34 with chemical compounds

  • At P26 and P34, in 50/10 rats that no longer had NV, retinal deltaPO(2)s during carbogen breathing were significantly (P < 0.05) lower than that of age-matched control pups [11].
  • The expression of sensitization, however, was partially overshadowed by an overall reduced sensitivity to cocaine seen in testing during the periadolescent period (i.e., at P34 and P41) [12].
  • Male offspring [postnatal day 29 (P29)-32] were tested for their ability to find a platform in a water maze before and after administration of a convulsant dose of pilocarpine at P34 [13].
  • The isolated 34 kDa protein (p34), when incorporated into a liposome model membrane, exhibited significant binding activity toward ribosomes, its binding properties being similar to those observed with intact RM [9].
  • ICI 182,780 administered to estradiol-treated ovariectomized rats blocked the induction of cdc2 p34 and the c-fos 28 kDa protein in the uterus [14].

Analytical, diagnostic and therapeutic context of P34

  • Primary sequence analysis, coupled with biochemical studies on the topology, suggested that p34 is a type II signal-anchor protein; it is composed of a large cytoplasmic domain, a membrane-spanning segment and a 38-amino-acid-long luminally disposed C-terminus [5].
  • Growth cone particle (GCP) membranes prepared from fetal day 17 rat brain are comprised of 5 major polypeptides as analyzed by SDS-PAGE: tubulin (p52), actin (p42), pp46/GAP-43 and two unidentified species, p38 and p34 [10].


  1. Synthesis of p34, the mammalian homolog of the yeast cdc2+/CDC28 protein kinase, is stimulated during adenovirus-induced proliferation of primary baby rat kidney cells. Draetta, G., Beach, D., Moran, E. Oncogene (1988) [Pubmed]
  2. Selective elimination of axons extended by developing cortical neurons is dependent on regional locale: experiments utilizing fetal cortical transplants. O'Leary, D.D., Stanfield, B.B. J. Neurosci. (1989) [Pubmed]
  3. Membrane proteins of the nerve growth cone and their developmental regulation. Simkowitz, P., Ellis, L., Pfenninger, K.H. J. Neurosci. (1989) [Pubmed]
  4. Fate of newborn dentate granule cells after early life status epilepticus. Porter, B.E., Maronski, M., Brooks-Kayal, A.R. Epilepsia (2004) [Pubmed]
  5. Ribosome-binding protein p34 is a member of the leucine-rich-repeat-protein superfamily. Ohsumi, T., Ichimura, T., Sugano, H., Omata, S., Isobe, T., Kuwano, R. Biochem. J. (1993) [Pubmed]
  6. Anti-(p34 protein) antibodies inhibit ribosome binding to and protein translocation across the rough microsomal membrane. Ichimura, T., Shindo, Y., Uda, Y., Ohsumi, T., Omata, S., Sugano, H. FEBS Lett. (1993) [Pubmed]
  7. Selective degradation of cyclin B1 mRNA in rat oocytes by RNA interference (RNAi). Lazar, S., Gershon, E., Dekel, N. J. Mol. Endocrinol. (2004) [Pubmed]
  8. Developmental expression of insulin-regulatable glucose transporter GLUT-4. Studelska, D.R., Campbell, C., Pang, S., Rodnick, K.J., James, D.E. Am. J. Physiol. (1992) [Pubmed]
  9. Isolation and some properties of a 34-kDa-membrane protein that may be responsible for ribosome binding in rat liver rough microsomes. Ichimura, T., Ohsumi, T., Shindo, Y., Ohwada, T., Yagame, H., Momose, Y., Omata, S., Sugano, H. FEBS Lett. (1992) [Pubmed]
  10. The p38 and p34 polypeptides of growth cone particle membranes are the alpha- and beta-subunits of G proteins. Edmonds, B.T., Moomaw, C.R., Hsu, J.T., Slaughter, C., Ellis, L. Brain Res. Dev. Brain Res. (1990) [Pubmed]
  11. Specificity of subnormal deltaPO2 for retinal neovascularization in experimental retinopathy of prematurity. Zhang, W., Ito, Y., Berlin, E., Roberts, R., Luan, H., Berkowitz, B.A. Invest. Ophthalmol. Vis. Sci. (2003) [Pubmed]
  12. Longevity of the expression of behavioral sensitization to cocaine in preweanling rats. Snyder, K.J., Katovic, N.M., Spear, L.P. Pharmacol. Biochem. Behav. (1998) [Pubmed]
  13. Protective effects of prenatal choline supplementation on seizure-induced memory impairment. Yang, Y., Liu, Z., Cermak, J.M., Tandon, P., Sarkisian, M.R., Stafstrom, C.E., Neill, J.C., Blusztajn, J.K., Holmes, G.L. J. Neurosci. (2000) [Pubmed]
  14. Differential effect of estrogen on the production of cyclin B1, cdc2 p34 and c-fos protein in rat uterus. Yang, L.S., Marshall, A., Koide, S.S. Endocr. Res. (1994) [Pubmed]
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