Disease relevance of IFT172

• STUDY OBJECTIVES: Presently, surgical (open or thoracoscopic) lung biopsy (SLB) is the gold standard for the diagnosis of new-onset idiopathic pulmonary fibrosis (IPF) and other interstitial lung diseases (ILDs) [1].
• T cells from a patient with malignant melanoma were infected with HTLV I by co-culture with a HTLV I-producing T cell line, SLB I [2].
• Mean maximal binding (Bmax) of the alpha2-adrenoceptor antagonist [3H]rauwolscine to platelet membranes was significantly reduced (P < 0.001) after exposure to chronic hypoxia [SLB: 172.6 (48.5) fmol x mg(-1) protein versus SLA: 136.8 (56.1) fmol x mg(-1) protein] without change in the dissociation constant (K(D)) [3].
• Forty-eight percent of SLB sera contained aAbs directed against the ribosomal proteins of the 60 S subunit compared to 9% for RA, 5% for blood donors and 0% for thyroiditis [4].

High impact information on IFT172

• We demonstrate that SLB specifically binds to Lhx3 and Lhx4 with high affinity both in vitro and in vivo [5].
• SLB is expressed in a tissue-specific manner with the highest concentrations of SLB mRNA in the testis and pituitary cells [5].
• The rate of DNA synthesis by human lymphocytes was studied in vitro by measuring unstimulated thymidine-2-14C incorporation (spontaneous lymphocyte blastogenesis; SLB) [6].
• Finally, perfusion of isolated buccal ganglia with SCPB excites the SLB cells and activates FMPs [7].
• In all species examined, the SLB cells project out the gastroesophageal nerves and appear to innervate the esophagus [7].

Biological context of IFT172

• An expression vector for a fragment of SLB containing the LIM-interaction domain was shown to reduce expression of Lhx3-responsive reporter genes [5].
• The sequence analysis of these fragments and the peptides by conventional methods established the complete amino acid sequence of SLB [8].
• A complement-dependent cytotoxic test showed the presence of antigens of the SLA major histocompatibility complex, the SLB leucocyte system and the A and E blood group systems on the granulocytes [9].

Anatomical context of IFT172

• Diurnal variations in the rate of DNA synthesis by circulating lymphocytes of healthy men were measured in vitro by SLB at 2-hr intervals for 24 hr [6].
• In each species, an apparent rhythmic feeding motor program (FMP) was observed by intracellular recording from both SLB neurons and other neurons in isolated preparations of the buccal ganglia [7].
• The data were consistent with Na+ and Cl- ions initially remaining inside the cell and a cell membrane Lp of 10(-3) cm sec-1 osm-1, which is close to the values determined by Spring and co-workers (K.R. Spring, A. Hope & B.E. Persson, 1981. In: Water Transport Across Epithelia. Alfred Benzon Symposium 15. pp. 190-200. Munskgaard, Copenhagen) [10].
• The system was designated SLB and its product (antigen SLB-A1) was demonstrated in all lymphocytes from the lymph nodes, thymus and peripheral blood, but not in the erythrocytes [11].

References