High impact information on PTGFR

• Northern blot analysis and in situ hybridization indicated that the PGF2 alpha receptor mRNA is highly expressed and accumulated in corpus luteum [1].
• Distinct mechanisms regulate induction of messenger ribonucleic acid for prostaglandin (PG) G/H synthase-2, PGE (EP3) receptor, and PGF2 alpha receptor in bovine preovulatory follicles [2].
• To examine the mechanisms underlying the effects of PGF2 alpha receptor desensitization on agonist-induced second messenger formation and contraction in bovine iris sphincter [3].
• In the isolated bovine iris sphincter, protein kinase C (PKC) is involved in the activation of adenylate cyclase and the desensitization of prostaglandin F2 alpha receptor-mediated responses supported by these findings [3].
• In this study we characterized LH and PGF2 alpha receptor (r) expression in the various luteal cell types using quantitative reverse transcription-polymerase chain reaction [4].

Biological context of PTGFR

• Assignment of the bovine PTGFR and HSD3B1 genes to bovine chromosome 3 with somatic and radiation hybrid panel mapping [5].
• CL from each of the 4 days of the estrous cycle were not different with respect to PGF2 alpha receptor concentration (number per microgram of plasma membrane protein) and affinity [6].
• Specific high affinity [3H]PGF2 alpha binding sites with Kd values of 18.3-31.1 nmol-1 were observed in the luteal membrane during the oestrous cycle and pregnancy; this is consistent with the expression of PGF2 alpha receptor mRNA [7].
• We have found that incubation of the sphincter with 25 microM PGF2 alpha for 45 min leads to: (a) significant loss in sensitivity of the tissue to PGF2 alpha receptor-stimulated inositol phosphates accumulation, PA production, MLC phosphorylation and contraction, and (b) significant increase in both basal and PGF2 alpha-stimulated cAMP formation [8].

Anatomical context of PTGFR

• Plasma membranes from individual CL were evaluated for PGF2 alpha receptor concentration and affinity by Scatchard analysis [6].
• Expression of mRNA encoding the prostaglandin F2 alpha receptor in bovine corpora lutea throughout the oestrous cycle and pregnancy [7].
• Tritiated prostaglandin F2 alpha ([3H]PGF2 alpha) binding to bovine corpora luteal membranes has been reexamined from the viewpoint of eventual PGF2 alpha receptor purification [9].
• Distribution of PGE and PGF2 alpha receptor proteins in the intracellular organelles of bovine corpora lutea [10].
• The effect of short-term prostaglandin (PG) desensitization on PGF2 alpha receptor-mediated inositol phosphates accumulation, 1,2-diacylglycerol production, measured as phosphatidic acid (PA), myosin light chain (MLC) phosphorylation, cAMP formation and contraction was investigated in bovine iris sphincter smooth muscle [8].

Associations of PTGFR with chemical compounds

• To investigate the regulation of ovine luteal receptors for prostaglandin F2 alpha (PGF2 alpha), reverse transcription-polymerase chain reaction was used to produce a 284-bp partial cDNA that was 98% identical to that reported for the bovine PGF2 alpha receptor (PGF2 alpha-R) [11].
• A prostaglandin F2 alpha receptor localized in plasma membranes of bovine corpus luteum cells was solubilized by treatment with Triton X-100 [12].
• These results demonstrate that the PGF2 alpha receptor is coupled to phosphoinositide metabolism in CHO-PGF2 alpha cells via Gq [13].

Other interactions of PTGFR

• Regulation of steady-state concentrations of messenger ribonucleic acid encoding prostaglandin F2 alpha receptor in ovine corpus luteum [11].

Analytical, diagnostic and therapeutic context of PTGFR

• Molecular cloning and expression of a cDNA of the bovine prostaglandin F2 alpha receptor [1].

References