Disease relevance of IGHE

• The results show that the IGHG3(b/b)-IGHG1(f/f)-IGHG2(n/n) genes are in linkage disequilibrium with allergen-specific high-responding IGHE genes and present the atopic phenotype of bronchial asthma, while the IGHG3(g/g)-IGHG1(a/a)-IGHG2(-n/-n) genes present the nonatopic phenotype of childhood asthma [1].
• Inhibition of the Prausnitz-Küstner reaction by an immunoglobulin epsilon-chain fragment synthesized in E. coli [2].

High impact information on IGHE

• Long terminal repeat-like elements flank a human immunoglobulin epsilon pseudogene that lacks introns [3].
• An immunoglobulin epsilon heavy chain gene was isolated from a DNA library of the human epsilon chain-producing myeloma 266B1 , using a JH gene region probe [4].
• There are at least three immunoglobulin epsilon genes (C epsilon 1, C epsilon 2, and C epsilon 3) in the human genome [3].
• Nucleotide sequences of immunoglobulin epsilon genes of chimpanzee and orangutan: DNA molecular clock and hominoid evolution [5].
• A recombinant human immunoglobulin epsilon-chain gene expression product (rFc epsilon) was compared with a human E myeloma protein in the affinity for epsilon-chain Fc fragment receptors (Fc epsilon R) on cultured human basophils [6].

Biological context of IGHE

• The IGHG genes are situated close to the IGHE gene on chromosome 14q32, 5'mu, delta, gamma3, gamma1, alpha1, gamma2, gamma4, epsilon, alpha2, 3'. They are inherited in a Mendelian fashion and expressed randomly in allelic exclusion [7].
• HindIII/EcoRI RFLP of the human immunoglobulin IGHE gene detected with a C epsilon probe in the French and Tunisian populations [8].
• A truncated immunoglobulin epsilon pseudogene is found in gorilla and man but not in chimpanzee [9].
• Characterization of the human immunoglobulin epsilon mRNAs and their polyadenylation sites [10].
• Multiple transcripts of the murine immunoglobulin epsilon membrane locus are generated by alternative splicing and differential usage of two polyadenylation sites [11].

Anatomical context of IGHE

• We detected the IGHE, IGHEP1 and IGHEP2 genes in all 55 leukocyte DNAs [12].
• Cloning and sequence determination of the gene for the human immunoglobulin epsilon chain expressed in a myeloma cell line [13].
• Treatment of splenic B lymphoid cells with LPS plus IL-4 induces the expression of transcripts from the germ line epsilon transcription unit followed by expression of normal immunoglobulin epsilon heavy-chain mRNA [14].
• One rate-limiting step is the class switch, by which B lymphocytes switch to produce immunoglobulin epsilon heavy chains rather than micro or gamma heavy chains [15].
• Inhibition of mast cell sensitization in vitro by a human immunoglobulin epsilon-chain fragment synthesized in Escherichia coli [16].

Associations of IGHE with chemical compounds

• In other studies, 3,3-dithiobis(sulfosuccinimidyl) propionate was used to cross-link hIgE to 125I surface-labeled 8866 cells [17].

Other interactions of IGHE

• The most common duplication, found in all populations studied, encompasses the IGHA1-IGHE genes [18].
• To determine if atopy could be associated with structural alterations involving the IGHE gene of the immunoglobulin heavy chain constant region, the genomic DNA from five atopic individuals was examined [19].
• Molecular analysis demonstrates that the T17 deletion occurred between highly homologous regions located downstream of IGHEP1 and IGHE, respectively [20].
• Genetic analysis of IgE and the IGHE, IGHEP1 and IGHEP2 genes in atopic families [12].
• We found that the deleted DNA includes the functional epsilon gene, and that the breakpoints are located within a 2 kilobase Bam HI/Sac I region of both the IGHEP1 and IGHE genes [21].

Analytical, diagnostic and therapeutic context of IGHE

• Comparative mapping of the immunoglobulin C epsilon 1 gene (IGHE) in five species of nonhuman primates by fluorescence in situ hybridization [22].
• Molecular cloning and nucleotide sequencing of human immunoglobulin epsilon chain cDNA [23].
• When the HBL cells were surface labeled with 125I and subsequently solubilized with non-ionic detergent, the labeled hIgE receptor could be isolated by either affinity chromatography on IgE-Sepharose (either human or rodent) or by immunoprecipitation with hIgE and anti-IgE [24].
• By site-directed mutagenesis we investigated the contribution of amino acids within the ecFcepsilonRIalpha FG loop (residues Lys154-Leu165) to binding to hIgE and two monoclonal anti-FcepsilonRIalpha antibodies (15/1, 5H5/F8) [25].

References