Disease relevance of DptB

• The dptlp gene was rapidly induced by bacterial infections and showed different time-dependent gene expression patterns from those of diptericin [1].
• The promoter of the single-copy, intronless diptericin gene of Drosophila contains several nucleotide sequences homologous to mammalian cis-regulatory motifs involved in the control of acute phase response genes [2].
• In this work we used the haemocyte-like mbn-2 cell line to investigate how expression of the antimicrobial peptide diptericin is affected after a high dose bacterial challenge with diaminopimelic acid (DAP)-peptidoglycan Gram-positive and Gram-negative bacteria [3].

High impact information on DptB

• Extending our previous studies on the expression of the diptericin gene, we now report a quantitative analysis of the contribution of various putative regulatory elements to the bacterial inducibility of this gene, based on the generation of 60 transgenic fly lines carrying different elements fused to a reporter gene [2].
• We propose that this motif cooperatively interacts with the other response elements in the regulation of the diptericin gene expression [4].
• Using a transfection assay of Drosophila tumorous blood cells, we show that the GAAANN sequence positively regulates the activity of the diptericin promoter [4].
• In addition, the dptlp gene expression was almost completely abolished in the imd mutant, which implicates that its expression is regulated by the anti-bacterial arm of the Drosophila innate immune regulatory pathways [1].
• Diptericin-like protein: an immune response gene regulated by the anti-bacterial gene induction pathway in Drosophila [1].

Biological context of DptB

• In support of this, we found GATA, interferon consensus responding element, and kappa B binding sites, which is known to be important for the proper expression of anti-bacterial genes, in the proximal promoter region of the dptlp gene [1].
• Insect immunity: the diptericin promoter contains multiple functional regulatory sequences homologous to mammalian acute-phase response elements [5].
• The activation of P. aeruginosa pexsCBA, the regulatory operon of the type III system, and the activation of the Drosophila gene diptericin, showed the host-pathogen recognition during infection process [6].
• Diptericin expression in bacteria infected Drosophila mbn-2 cells - effect of infection dose and phagocytosis [3].
• We report that diptericin expression is negatively affected by high infection dose and rapid bacterial growth regardless of the type of infection and bacterial virulence and occurs in the absence of mbn-2 cell death [3].

Anatomical context of DptB

• Like diptericin, dptlp was specifically produced from the fat body, and its expression was strictly dependent on bacterial infections [1].

Associations of DptB with chemical compounds

• Furthermore, isolated tubules bind and respond to lipopolysaccharide (LPS), by upregulating anti-microbial peptide (diptericin) gene expression and increased bacterial killing [7].

Analytical, diagnostic and therapeutic context of DptB

• Expression of the gene encoding diptericin, an antibacterial peptide in various D. melanogaster strains parasitized by several species of parasitoid wasps, was studied by Northern blot [8].

References