High impact information on h4-A

• Stable minichromosomes are also assembled on a cloned histone H4 gene; again, intragenic DNA rearrangements affect the efficiency of assembly of active chromatin and differential gene expression occurs after coinjection of two or more H4 DNA constructs [1].
• Methylation of histone H4 at arginine 3 facilitating transcriptional activation by nuclear hormone receptor [2].
• Histone H4 acetylation and transcription in amphibian chromatin [3].
• Injection of 19- or 20-mers complementary to 70-kd heat shock protein, histone H4 and vegetally localized Veg 1 coding sequences causes rapid cleavage and degradation of up to 96% of the target transcripts present in stage VI oocytes [4].
• The purified S6 protein kinase did not phosphorylate at a significant rate ribosomal protein S10, histone H1, histone H4, mixed histones, casein, or phosvitin, indicating a high degree of substrate specificity [5].

Biological context of h4-A

• Histone H4 mRNA synthesized from plasmid templates microinjected into oocyte nuclei is translationally silenced (masked) [6].
• Transcriptionally active Xenopus laevis somatic 5 S ribosomal RNA genes are packaged with hyperacetylated histone H4, whereas transcriptionally silent oocyte genes are not [7].
• The histone H4 acetyltransferase MOF uses a C2HC zinc finger for substrate recognition [8].
• Chromatin transitions during early Xenopus embryogenesis: changes in histone H4 acetylation and in linker histone type [9].
• In addition, we show that, unlike histone H4, Xenopus maternal histone H3 is stored in the oocyte as a hypoacetylated form and is rapidly acetylated when the oocyte exits meiosis [10].

Anatomical context of h4-A

• In oocytes and embryos up to the tailbud stage, four types of mRNAs complementary to histone H2B DNA and two complementary to histone H4 DNA can be discriminated by their different electrophoretic mobilities on polyacrylamide gels [11].
• Chromatin fragments from a X. laevis kidney cell line were immunoprecipitated with an antibody specific for hyperacetylated histone H4 [7].
• Level of histone H4 mRNA in Xenopus laevis embryonic cells cultured in the absence of cell adhesion [12].
• Symmetrical dimethyl arginine 3 of histone H4, a modification catalyzed by PRMT7, accumulates in germ cells during this developmental period [13].

Associations of h4-A with chemical compounds

• We examine the translational regulation of histone H4 mRNA when Xenopus laevis oocytes are induced to mature with progesterone [6].
• Addition of T3 results in histone H4 acetylation specifically on T3-response genes [14].

Regulatory relationships of h4-A

• We found the conserved N termini (the N-terminal tails) of histone H4 essential to stimulate ISWI ATPase activity, in contrast to other histone tails [15].
• The Y-box protein FRGY2 inhibits translation of histone H4 mRNA in vitro [6].

Other interactions of h4-A

• We have shown that these proteins are indeed nuclear basic proteins: the 14 kd is the histone H4, the 19 kd is the histone H3, and the 25 kd is the sperm-specific protein SP2 [16].
• Critical role for the histone H4 N terminus in nucleosome remodeling by ISWI [15].

Analytical, diagnostic and therapeutic context of h4-A

• They were located by in situ hybridization of a 3H-labelled histone H4 anti-sense cRNA probe applied to lampbrush preparations in which transcript RNA had been retained, and likewise to preparations in which transcripts were absent but whose DNA had been denatured prior to hybridization [17].
• The amount of histone H4 mRNA per embryo was followed during early development of Xenopus laevis by Northern blot analyses using a cloned histone H4 cDNA as the probe [12].

References