Disease relevance of LOC395926

• An H. pylori-positive 53-year-old female gastritis patient administered anti-HpU IgY plus lansoprazole for 8 weeks showed a decrease in serum pepsinogen (PG) I and UBT values as well as an increase in the PG I/II ratio [1].

High impact information on LOC395926

• For this we produced constructs containing various portions of the 5'-flanking region of the embryonic chick pepsinogen gene, driving reporter sequences (beta-galactocidase or luciferase), and these constructs were transfected into dissociated epithelial cells either from the proventriculus or gizzard [2].
• There is some considerable evidence in favour of epithelial-mesenchymal interactions being important during the development of the gastrointestinal system; for example, pepsinogen expression is induced in proventricular and gizzard (muscular stomach) epithelial by the proventricular mesenchyme but is suppressed by the gizzard mesenchyme [2].
• Mesenchymal regulation of epithelial gene expression in developing avian stomach: 5'-flanking region of pepsinogen gene can mediate mesenchymal influence on its expression [2].
• However, 6-day small intestinal or 3.5-day allantoic endoderm did not produce pepsinogen mRNA under the same conditions, though the recombinates formed complex glands [3].
• Pepsinogen gene transcription induced in heterologous epithelial-mesenchymal recombinations of chicken endoderms and glandular stomach mesenchyme [3].

Biological context of LOC395926

• The almost complete nucleotide sequence of embryonic chicken pepsinogen-cDNA was determined by sequencing longer cDNAs obtained by screening the same library with the 200-base pair cDNA and primer extension with a synthetic primer [4].
• To learn the phylogeny of this pepsinogen, we isolated a cDNA clone by screening a lambda gt11 library of embryonic proventricular cDNAs with an antiserum to the embryonic chicken pepsinogen [4].
• The complete amino acid sequence of monkey pepsinogen A [5].
• Embryonic chicken pepsinogen gene was isolated from a chicken genomic library [6].
• Developmental changes of DNA methylation pattern of embryonic chick pepsinogen gene [7].

Anatomical context of LOC395926

• In the development of chicken embryonic stomach, the epithelium invaginates into the mesenchyme and forms glands and gland epithelial cells then begin to express embryonic chicken pepsinogen (ECPg) gene [8].
• Embryonic chicken pepsinogen is an aspartyl proteinase that is specifically secreted during the embryonic period in the chicken proventriculus (glandular stomach) [4].
• The glandular epithelial cells produce pepsinogen, while the non-glandular (luminal) epithelial cells secrete mucus [9].
• Cytodifferentiation of the oxyntic cells was established by ultrastructural methods, while the expression of pepsinogen, mitochondrial enzyme markers and apical secretory membranes was determined by histochemical and biochemical procedures [10].
• Presence of pepsinogens immunoreactive to anti-embryonic chicken pepsinogen antiserum in fish stomachs: possible ancestor molecules of chymosin of higher vertebrates [11].

Associations of LOC395926 with chemical compounds

• Three disulfide bridges and 1 phosphoserine residue were found to be present in the pepsinogen molecule [5].
• After converting the pepsinogen to pepsin by activation, the pepsin moiety was reduced and carboxymethylated, cleaved by cyanogen bromide, and the amino acid sequences of the major fragments determined [5].
• Chicken pepsinogen was incubated at pH2.5 with pepstatin [12].
• Chicken pepsinogen is a glycoprotein consisting of a single polypeptide chain and containing the following 367 amino acid residues: Asp23, Asn16, Thr26, Ser41, Glu14, Gln11, Pro18, Gly31, Ala17, Cys7, Val25, Met9, Ile23, Leu28, Tyr22, Phe20, His8, Lys17, Arg7, Trp4 [13].
• An embryo-specific pepsinogen was isolated from the proventriculi of 15-day-old chicken embryos and purified by means of fractionation with ammonium sulfate, filtration on Sephadex G-100, and chromatography on DEAE-Sepharose CL-6B and hydroxyapatite [14].

Analytical, diagnostic and therapeutic context of LOC395926

• Molecular cloning and the nucleotide sequence of cDNA for embryonic chicken pepsinogen: phylogenetic relationship with prochymosin [4].
• Stomachs of 14 species selected from five classes of vertebrate were surveyed concerning the reactivity to an anti-adult chicken pepsinogen antiserum (anti-ACPg) with indirect immunofluorescence method [15].

References