High impact information on LEPR

• Using immunofluorescence microscopy, we demonstrated a polarized expression pattern of leptin receptor protein on the surface of mononuclear cells and constitutive expression of GH in PBMCs [1].
• We have previously shown that murine recombinant leptin directly stimulates catecholamine synthesis through the long form of the leptin receptor (Ob-Rb) expressed in cultured porcine chromaffin cells [2].
• The long form leptin receptor (Ob-Rl) is localized within the hypothalamus along with several neuropeptides that are involved in regulation of the neuroendocrine axis [3].
• Developmental changes in the long form leptin receptor and related neuropeptide gene expression in the pig brain [3].
• In addition, embryonic Lepr-L mRNA levels were higher in GT than in YL sows, and B(9) + glycine dietary supplement decreased the mRNA expression levels of Lep in backfat and of Lepr in embryonic tissues [4].

Biological context of LEPR

• The leptin receptor gene (LEPR) maps to porcine chromosome 6 [5].
• The aims of this work were: (i) to fine map these QTL on a larger sample of animals and generations (F3 and backcross) of an Iberian x Landrace intercross and (ii) to examine the effects of LEPR alleles on body composition traits [6].
• Eleven single nucleotide polymorphisms (SNPs) were detected by sequencing LEPR coding regions in Iberian and Landrace pig samples [6].
• Missense mutations in exon 4 of the porcine LEPR gene encoding extracellular domain and their association with fatness traits [7].
• The QTL mapping analysis was repeated with a regression method using genotypes for seven microsatellites and two PCR-RFLPs in the H-FABP and LEPR genes [8].

Anatomical context of LEPR

• Long form leptin receptor mRNA expression in the hypothalamus and pituitary during early pregnancy in the pig [9].
• Leptin receptor immunoreactivity (OBR-IR) was observed in both the preoptic area and anterior hypothalamic area [10].
• One of its targets in the central nervous system appears to be the epithelial cells of the choroid plexus where leptin receptor (OB-R) expression is particularly high [11].
• The expression of long-form leptin receptor mRNA was detected in fetal adipose tissue [12].
• The expression of the functional long form leptin receptor (Ob-Rb) mRNA was examined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) in follicular cells cultured with GH or IGF-I [13].

Associations of LEPR with chemical compounds

• The objectives of this study were to characterize the Lep, Lepr, and long Lepr-L isoform mRNA expression according to the breed and parity of gestating sows or to specific folic acid (B(9)) + glycine dietary treatments [4].
• During pubertal development serum leptin levels, hypothalamic leptin receptor mRNA and estrogen-induced leptin gene expression in fat increased with age and adiposity in the pig and this occurred at the time of expected puberty [14].
• Gh and IGF-I increase leptin receptor expression in prepubertal pig ovaries: the role of leptin in steroid secretion and cell apoptosis [13].

Other interactions of LEPR

• When an animal model was fitted, both genes showed significant effects on fatness traits, the H-FABP polymorphism showed significant effects on IMF and MA, and the LEPR polymorphism on BF and IMF [8].
• Leptin and long form of leptin receptor genes expression in the hypothalamus and pituitary during the luteal phase and early pregnancy in pigs [15].

Analytical, diagnostic and therapeutic context of LEPR

• HpaII and RsaI PCR-RFLPs within an intron of the porcine leptin receptor gene (LEPR) and its linkage mapping [16].
• The expression of short from of leptin receptor gene during early pregnancy in the pig examined by quantitative real time RT-PCR [17].

References