Disease relevance of LIMS1

• Surgical Pearl: Pinch-punch excisions for scrotal calcinosis [1].
• METHODS AND RESULTS: Pinch samples of the terminal ileum, proximal and distal colon were taken from a healthy 35-year-old, and a 68-year-old subject with mild diverticulosis [2].
• STUDY DESIGN: Pinch motion between the thumb and the index finger in myelopathy hand was analyzed using micro-reflective markers and the VICON system [3].

High impact information on LIMS1

• Consequently, loss of the PINCH-1-ILK complex is disastrous for embryogenesis in vertebrates and invertebrates [4].
• Molecular dissection of PINCH-1 reveals a mechanism of coupling and uncoupling of cell shape modulation and survival [5].
• The C-terminal tail not only regulates PINCH-1 localization to focal adhesions but also functions after it localizes there [5].
• The coordinated down-regulation of PINCH-1, ILK, and alpha-parvin proteins is mediated at least in part by proteasomes [6].
• Fourth, PINCH-1 and ILK function in the survival pathway not only upstream but also downstream (or in parallel) of protein kinase B (PKB)/Akt [6].

Biological context of LIMS1

• We have used RNA interference, a powerful approach of reverse genetics, to investigate the functions of PINCH-1 and ILK in human cells [6].
• Second, PINCH-1 and ILK, like alpha-parvin, are crucial for cell survival [6].
• Both these technologies illustrate the requirement to integrate molecular biology, automation, LIMS and data analysis [7].
• A set of bioinformatics tools, which are implemented in our LIMS, is utilized to analyze each target [8].
• We hope that many research scientists working in the field of proteomics will evaluate our LIMS and suggest ways in which it can be improved [9].

Anatomical context of LIMS1

• This study reviews our experience in less invasive mitral valve surgery (LIMS) during that time [10].

Regulatory relationships of LIMS1

• Treatment with an ILK inhibitor or disruption of the ILK/PINCH-1 interaction by overexpressing a dominant-negative N-terminal ankyrin domain of ILK resulted in reduced fibronectin deposition, indicating that the ability of PINCH-1 to stimulate EMT is ILK-dependent [11].

Other interactions of LIMS1

• These results identify Rac as a downstream target of PINCH-1, ILK, and parvin [12].
• The Pinch Test, and with it, the Bonney Test, are analysed with reference to the 1st and 2nd closure mechanisms presented in the Theory of Female Urinary Incontinence described earlier in this issue [13].

Analytical, diagnostic and therapeutic context of LIMS1

• Currently, storage for trials at 277 K is not automated and for imaging the crystallization plates are fed by hand into an imaging system from which the data enter the LIMS [14].
• The various processes all report electronically to a Laboratory Information Management System (LIMS) to make sure all the parameters of the experiment are recorded in an accessible and 'mineable' form, helping guarantee reproducibility of results [15].
• SUBJECTS AND METHODS: Fifty normal subjects and Twenty-one patients, (Eleven patients treated with early active and resistive goal directed mobilisation and Ten control group) were assessed for pinch strength and grip strength on Pinch Dynamometer and Jamar Dynamometer and work of hand muscles on Ergograph [16].
• Rejuvenation may be reached by simple hardware and software modifications, to allow data exchange with modern PC's and LIMS [17].
• In our laboratory we developed a Laboratory Information Management System (LIMS) controlled workflow architecture, which comprises a robotic DNA extraction- and pipetting-system and a capillary electrophoresis unit [18].

References