The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

ILK  -  integrin-linked kinase

Homo sapiens

Synonyms: 59 kDa serine/threonine-protein kinase, HEL-S-28, ILK-1, ILK-2, ILK1, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of ILK

  • Western blot analyses indicated very low levels of ParvB protein in MDA-MB-231 and MCF7 breast cancer cells, facilitating functional studies of the effects of ParvB on ILK signaling [1].
  • Our initial results indicate that therapeutic strategies targeting ILK may be beneficial in the treatment of glioblastomas [2].
  • To determine the role of ILK in the cellular radiation response, stably transfected A549 lung cancer cells overexpressing either wild-type (ILK-wk) or hyperactive ILK (ILK-hk) were studied for survival, signaling, proliferation, and examined in immunofluorescence and adhesion assays [3].
  • These results demonstrate that alpha(v) integrins regulate ovarian cancer cell proliferation through ILK [4].
  • In addition, ILK activity and expression are elevated in adenomatous polyposis and colon carcinomas [5].

High impact information on ILK


Chemical compound and disease context of ILK


Biological context of ILK

  • Furthermore, dominant-negative ILK or WT PTEN induces G(1) phase cycle arrest and enhanced apoptosis [13].
  • Transfection of dominant-negative ILK and WT PTEN into these cells also results in the inhibition of PKB/Akt kinase activity [13].
  • Inhibition of ILK suppressed this LPS-stimulated IkappaB phosphorylation and degradation [14].
  • Here we demonstrate, using several different approaches, that inhibiting ILK kinase activity, or expression, results in the inhibition of cell attachment, cell migration, F-actin organization, and the specific cytoskeletal localization of CH-ILKBP and paxillin in human cells [15].
  • These data demonstrate that the kinase and adaptor properties of ILK function together, in a Pi3 kinase-dependent manner, to regulate integrin-mediated cell attachment and signal transduction [15].

Anatomical context of ILK


Associations of ILK with chemical compounds

  • We recently have demonstrated that the integrin-linked kinase (ILK) can phosphorylate PKB/Akt on Ser-473 in a phosphoinositide phospholipid-dependent manner [13].
  • Genetic studies in Drosophila, Caenorhabditis elegans, and mice point to an essential role of ILK as an adaptor protein in mediating integrin-dependent cell attachment and cytoskeletal organization [15].
  • The amino acid residue serine 343 of ILK within the activation loop is required for kinase activity as well as for its interaction with PKB/Akt [21].
  • Mutational analysis of ILK further shows a crucial role for arginine 211 of ILK within the phosphoinositide phospholipid binding domain in the regulation of PKB- serine 473 phosphorylation [21].
  • These results indicated that ParvB inhibits ILK signaling downstream of receptor tyrosine kinases [1].
  • The PINCH LIM1-2 fragment inhibited the recruitment of endogenous ILK and PINCH to integrin adhesion sites and prevented their association of ILK with beta-integrins, paxillin, and vinculin [22].

Physical interactions of ILK


Enzymatic interactions of ILK


Regulatory relationships of ILK


Other interactions of ILK


Analytical, diagnostic and therapeutic context of ILK


  1. Beta-parvin inhibits integrin-linked kinase signaling and is downregulated in breast cancer. Mongroo, P.S., Johnstone, C.N., Naruszewicz, I., Leung-Hagesteijn, C., Sung, R.K., Carnio, L., Rustgi, A.K., Hannigan, G.E. Oncogene (2004) [Pubmed]
  2. Inhibition of ILK in PTEN-mutant human glioblastomas inhibits PKB/Akt activation, induces apoptosis, and delays tumor growth. Edwards, L.A., Thiessen, B., Dragowska, W.H., Daynard, T., Bally, M.B., Dedhar, S. Oncogene (2005) [Pubmed]
  3. Overexpression of hyperactive integrin-linked kinase leads to increased cellular radiosensitivity. Cordes, N. Cancer Res. (2004) [Pubmed]
  4. alpha(v) integrins regulate cell proliferation through integrin-linked kinase (ILK) in ovarian cancer cells. Cruet-Hennequart, S., Maubant, S., Luis, J., Gauduchon, P., Staedel, C., Dedhar, S. Oncogene (2003) [Pubmed]
  5. Inhibition of integrin linked kinase (ILK) suppresses beta-catenin-Lef/Tcf-dependent transcription and expression of the E-cadherin repressor, snail, in APC-/- human colon carcinoma cells. Tan, C., Costello, P., Sanghera, J., Dominguez, D., Baulida, J., de Herreros, A.G., Dedhar, S. Oncogene (2001) [Pubmed]
  6. Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase. Hannigan, G.E., Leung-Hagesteijn, C., Fitz-Gibbon, L., Coppolino, M.G., Radeva, G., Filmus, J., Bell, J.C., Dedhar, S. Nature (1996) [Pubmed]
  7. Integrin-linked kinase: integrin's mysterious partner. Grashoff, C., Thievessen, I., Lorenz, K., Ussar, S., Fässler, R. Curr. Opin. Cell Biol. (2004) [Pubmed]
  8. RNA interference demonstrates a novel role for integrin-linked kinase as a determinant of pancreatic adenocarcinoma cell gemcitabine chemoresistance. Duxbury, M.S., Ito, H., Benoit, E., Waseem, T., Ashley, S.W., Whang, E.E. Clin. Cancer Res. (2005) [Pubmed]
  9. ILK (beta1-integrin-linked protein kinase): a novel immunohistochemical marker for Ewing's sarcoma and primitive neuroectodermal tumour. Chung, D.H., Lee, J.I., Kook, M.C., Kim, J.R., Kim, S.H., Choi, E.Y., Park, S.H., Song, H.G. Virchows Arch. (1998) [Pubmed]
  10. Involvement of integrin-linked kinase in carbon tetrachloride-induced hepatic fibrosis in rats. Zhang, Y., Ikegami, T., Honda, A., Miyazaki, T., Bouscarel, B., Rojkind, M., Hyodo, I., Matsuzaki, Y. Hepatology (2006) [Pubmed]
  11. Integrin-linked kinase is an essential link between integrins and uptake of bacterial pathogens by epithelial cells. Wang, B., Yurecko, R.S., Dedhar, S., Cleary, P.P. Cell. Microbiol. (2006) [Pubmed]
  12. Integrin-linked kinase functions as a downstream mediator of endothelin-1 to promote invasive behavior in ovarian carcinoma. Rosanò, L., Spinella, F., Di Castro, V., Dedhar, S., Nicotra, M.R., Natali, P.G., Bagnato, A. Mol. Cancer Ther. (2006) [Pubmed]
  13. Inhibition of integrin-linked kinase (ILK) suppresses activation of protein kinase B/Akt and induces cell cycle arrest and apoptosis of PTEN-mutant prostate cancer cells. Persad, S., Attwell, S., Gray, V., Delcommenne, M., Troussard, A., Sanghera, J., Dedhar, S. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  14. Integrin-linked kinase regulates inducible nitric oxide synthase and cyclooxygenase-2 expression in an NF-kappa B-dependent manner. Tan, C., Mui, A., Dedhar, S. J. Biol. Chem. (2002) [Pubmed]
  15. Integration of cell attachment, cytoskeletal localization, and signaling by integrin-linked kinase (ILK), CH-ILKBP, and the tumor suppressor PTEN. Attwell, S., Mills, J., Troussard, A., Wu, C., Dedhar, S. Mol. Biol. Cell (2003) [Pubmed]
  16. Integrin-linked kinase regulates vascular morphogenesis induced by vascular endothelial growth factor. Kaneko, Y., Kitazato, K., Basaki, Y. J. Cell. Sci. (2004) [Pubmed]
  17. Integrin-linked kinase phosphorylates the myosin phosphatase target subunit at the inhibitory site in platelet cytoskeleton. Kiss, E., Murányi, A., Csortos, C., Gergely, P., Ito, M., Hartshorne, D.J., Erdodi, F. Biochem. J. (2002) [Pubmed]
  18. Gene microarray analysis reveals a novel hypoxia signal transduction pathway in human hepatocellular carcinoma cells. Scandurro, A.B., Weldon, C.W., Figueroa, Y.G., Alam, J., Beckman, B.S. Int. J. Oncol. (2001) [Pubmed]
  19. The gamma-parvin-integrin-linked kinase complex is critically involved in leukocyte-substrate interaction. Yoshimi, R., Yamaji, S., Suzuki, A., Mishima, W., Okamura, M., Obana, T., Matsuda, C., Miwa, Y., Ohno, S., Ishigatsubo, Y. J. Immunol. (2006) [Pubmed]
  20. Integrin-linked kinase (ILK) binding to paxillin LD1 motif regulates ILK localization to focal adhesions. Nikolopoulos, S.N., Turner, C.E. J. Biol. Chem. (2001) [Pubmed]
  21. Regulation of protein kinase B/Akt-serine 473 phosphorylation by integrin-linked kinase: critical roles for kinase activity and amino acids arginine 211 and serine 343. Persad, S., Attwell, S., Gray, V., Mawji, N., Deng, J.T., Leung, D., Yan, J., Sanghera, J., Walsh, M.P., Dedhar, S. J. Biol. Chem. (2001) [Pubmed]
  22. Integrin-linked kinase regulates N-WASp-mediated actin polymerization and tension development in tracheal smooth muscle. Zhang, W., Wu, Y., Wu, C., Gunst, S.J. J. Biol. Chem. (2007) [Pubmed]
  23. The role of integrin-linked kinase (ILK) in cancer progression. Persad, S., Dedhar, S. Cancer Metastasis Rev. (2003) [Pubmed]
  24. A new focal adhesion protein that interacts with integrin-linked kinase and regulates cell adhesion and spreading. Tu, Y., Huang, Y., Zhang, Y., Hua, Y., Wu, C. J. Cell Biol. (2001) [Pubmed]
  25. The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells. Tu, Y., Li, F., Goicoechea, S., Wu, C. Mol. Cell. Biol. (1999) [Pubmed]
  26. PP2C family members play key roles in regulation of cell survival and apoptosis. Tamura, S., Toriumi, S., Saito, J., Awano, K., Kudo, T.A., Kobayashi, T. Cancer Sci. (2006) [Pubmed]
  27. Integrin-linked kinase (ILK): a "hot" therapeutic target. Yoganathan, T.N., Costello, P., Chen, X., Jabali, M., Yan, J., Leung, D., Zhang, Z., Yee, A., Dedhar, S., Sanghera, J. Biochem. Pharmacol. (2000) [Pubmed]
  28. Signaling through beta-catenin and Lef/Tcf. Novak, A., Dedhar, S. Cell. Mol. Life Sci. (1999) [Pubmed]
  29. The parvins. Sepulveda, J.L., Wu, C. Cell. Mol. Life Sci. (2006) [Pubmed]
  30. Promoter characterization and genomic organization of the gene encoding integrin-linked kinase 1. Melchior, C., Kreis, S., Janji, B., Kieffer, N. Biochim. Biophys. Acta (2002) [Pubmed]
  31. ILKAP regulates ILK signaling and inhibits anchorage-independent growth. Kumar, A.S., Naruszewicz, I., Wang, P., Leung-Hagesteijn, C., Hannigan, G.E. Oncogene (2004) [Pubmed]
  32. Integrin-linked kinase interacts with caspase-9 and -8 in an adhesion-dependent manner for promoting radiation-induced apoptosis in human leukemia cells. Hess, F., Estrugo, D., Fischer, A., Belka, C., Cordes, N. Oncogene (2007) [Pubmed]
  33. Conditional knock-out of integrin-linked kinase demonstrates an essential role in protein kinase B/Akt activation. Troussard, A.A., Mawji, N.M., Ong, C., Mui, A., St -Arnaud, R., Dedhar, S. J. Biol. Chem. (2003) [Pubmed]
  34. Distinct roles of two structurally closely related focal adhesion proteins, alpha-parvins and beta-parvins, in regulation of cell morphology and survival. Zhang, Y., Chen, K., Tu, Y., Wu, C. J. Biol. Chem. (2004) [Pubmed]
  35. Molecular dissection of actopaxin-integrin-linked kinase-Paxillin interactions and their role in subcellular localization. Nikolopoulos, S.N., Turner, C.E. J. Biol. Chem. (2002) [Pubmed]
  36. Cloning of an isoform of integrin-linked kinase (ILK) that is upregulated in HT-144 melanoma cells following TGF-beta1 stimulation. Janji, B., Melchior, C., Vallar, L., Kieffer, N. Oncogene (2000) [Pubmed]
  37. Integrin-linked kinase acts as a pro-survival factor against high glucose-associated osmotic stress in human mesangial cells. Ohnishi, M., Hasegawa, G., Yamasaki, M., Obayashi, H., Fukui, M., Nakajima, T., Ichida, Y., Ohse, H., Mogami, S., Yoshikawa, T., Nakamura, N. Nephrol. Dial. Transplant. (2006) [Pubmed]
WikiGenes - Universities