Disease relevance of UMOD

• In summary our data give evidence that renal tubular cell THP expression may be relevant in kidney diseases since THP is a potent activator of human granulocytes [1].
• In the present study, we assessed levels of retinol, retinyl esters, retinol-binding protein (RBP) and THP in plasma and urine of dogs with a history of urolithiasis (n = 25) compared with clinically healthy controls (n = 18) [2].
• OBJECTIVES: To study the role of urinary inhibitors on crystallization-nephrocalcin, Tamm-Horsfall protein (THP), and glycosaminoglycans (GAGs)-in hyperuricosuric nephrolithiasis using Dalmatian dogs, a breed well-known for high levels of uric acid excretion, in an in vivo model [3].

High impact information on UMOD

• The relative potency was PCE greater than TCP greater than PCP greater than THP greater than PCDEA greater than ketamine [4].
• Coated COM crystals from male controls contained 3.5-fold more Tamm-Horsfall protein (THP) than SF subjects (P < 0.01) [5].
• A strong binding of THP to human granulocytes was demonstrated by fluorescence-activated cell sorter analysis [1].
• Renal tubular epithelial cell mechanisms inducing granulocytic activation and bacterial killing may include tubular cell expression of Tamm-Horsfall protein (THP), a urinary protein that is known to enhance cytokine expression in monocytes [1].
• Renal tubular cells growing polarized on polycarbonate membranes were used to study apical versus basal THP expression [1].

Biological context of UMOD

• Canine umod has been mapped on the RHDF5000 radiation hybrid panel and positioned on the most recent canine genome map [6].
• The cDNA is 2.3 kb in length and, as expected, comparisons of nucleotide sequences reveal that canine umod is quite similar to umod of other mammals [6].
• Data indicate that umod is linked to the marker CZP2 (canine zona pellucida gene) on an RH group not yet assigned to a canine chromosome [6].
• Urinary THP excretion, glomerular filtration rate, renal blood flow, mean arterial pressure, and renal venous malondialdehyde concentrations were measured before and after administration of contrast medium to each kidney [7].
• Peptides obtained after limited proteolysis with trypsin from the 100 kDa protein were characterised by MALDI-TOF mass spectrometry and showed amino acid sequence homology to the human Tamm-Horsfall Protein (THP) [8].

Anatomical context of UMOD

• By electron microscopy THP immunoreactivity was exclusively found on the apical surfaces of tubular cells and was absent on the basolateral cell membrane [1].
• THP immunoreactivity was seen in the epithelial cells lining the thick ascending limb of the loop of Henle [9].

Associations of UMOD with chemical compounds

• During the postcontrast period, SOD significantly attenuated the increase in urinary THP excretion, accompanied by an attenuated increase in renal venous malondialdehyde concentration [7].
• The use of another contrast medium, iothalamate sodium, similarly increased urinary THP excretion [7].
• Our data point to the possibility that THP functions as a novel carrier for vitamin A in the urine of canines [8].
• OBJECTIVE: To determine plasma and urine concentrations of retinol, retinyl esters, retinol-binding protein (RBP), and Tamm-Horsfall protein (THP) in dogs with chronic renal disease (CRD) [10].
• Excretion of urinary retinol and retinyl esters were tentatively, but not significantly higher in the stone-forming groups, which was accompanied by increased levels of urinary RBP (P < 0.01) and lower excretions in THP (P < 0.01) [2].

Analytical, diagnostic and therapeutic context of UMOD

• Concentrations of RBP and THP were measured by use of sensitive ELISA systems [10].
• Sodium dodecyl sulphate-polyacrylamid gel electrophoresis and subsequent Western blot analysis of urine showed the presence of heavy and light chains of immunoglobulin, transferrin, albumin, vitamin D-binding protein, transthyretin and retinol-binding protein (RBP), but no excretion of Tamm-Horsfall protein (THP) [11].
• The presence of THP, the carrier for vitamin A in canine urine, showed that vitamin A excretion in the urine of dogs is not merely a filtration process but also seems to be a pathway located in the distal part of the nephron [9].

References