High impact information on alpha-Est5

• Esterase-5 allozyme classes based on electrophoresis in 5% acrylamide gels are therefore highly heterogeneous [1].
• Molecular evolution of the Sex-Ratio inversion complex in Drosophila pseudoobscura: analysis of the Esterase-5 gene region [2].
• A 504-bp fragment of noncoding, intergenic DNA from the esterase-5 gene region was amplified and sequenced from 14 Sex-Ratio and 14 Standard X chromosomes of D. pseudoobscura, and from 9 X chromosomes of its two sibling species, Drosophila persimilis and Drosophila miranda [2].
• A clone of the esterase-5 (Est-5) gene from Drosophila pseudoobscura has been isolated by hybridization to the cloned Est-6 gene of D. melanogaster [3].
• The extremely close similarity in frequency distributions of the alleles between populations for both the xanthine dehydrogenase and esterase-5 loci, despite differences in allele frequency distribution between loci, strongly emphasizes the importance of migration in influencing genic diversity in these populations [4].

Biological context of alpha-Est5

• Genetic diversity was depressed within esterase 5 within Sex-ratio chromosomes, while the four other genes failed to show a reduction in heterozygosity in the Sex-ratio background [5].
• Electrophoretic phenotypes of the esterase-5 locus were examined in the sibling species D. pseudoobscura, D. persimilis and D. miranda [6].
• This article presents a nucleotide sequence analysis of 500 bp determined in each of five X-linked genes, runt, sisterlessA, period, esterase 5, and Heat-shock protein 83, in 40 Drosophila pseudoobscura strains collected from two populations [5].
• Interphase but not mitotic phosphorylation was found on an internal CNBr fragment (derived from the end of the central rod domain and the first part of the C-terminal lamin tail; amino acid residues 385-548; fragment D) [7].

Anatomical context of alpha-Est5

• Disc gel electrophoresis of dissected adults of Drosophila pseudoobscura showed that most of the esterase-5 activity was in the head (36%) and thorax (51%), with little activity in the abdomen (13%) [8].

Other interactions of alpha-Est5

• Esterase-4 and esterase-5 are the products of a duplicated gene [9].
• Final purification was achieved by anion-exchange h.p.l.c., in the case of esterase-5 followed by gel-filtration h.p.l.c. The resultant esterase preparations were homogeneous, as judged by gel-filtration h.p.l.c., SDS/polyacrylamide-gel electrophoresis and non-denaturing gel electrophoresis [9].
• A number of separate strains of Drosophila pseudoobscura were inbred for 38 generations of brother-sister mating with forced heterozygosity for two alleles of either the octanol dehydrogenase or esterase-5 locus [10].

Analytical, diagnostic and therapeutic context of alpha-Est5

• Study of the charge-state model for electrophoretic variation using isoelectric focusing of esterase-5 from Drosophila pseudoobscura [11].

References