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Gene Review

MANBA  -  mannosidase, beta A, lysosomal

Homo sapiens

Synonyms: Beta-mannosidase, Lysosomal beta A mannosidase, MANB1, Mannanase, Mannase
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Disease relevance of MANBA


High impact information on MANBA

  • This MANB-GPI linkage and confirming chromosome studies demonstrate assignment of the alpha-mannosidase B structural gene to chromosome 19 in man [6].
  • Chromosome assignment of the gene coding for human alpha-mannosidase B (MANB) has been determined in human-mouse and human-Chinese hamster somatic cell hybrids [6].
  • Two cDNAs, designated xynA and manA, encoding xylanase A (XYLA) and mannanase A (MANA), respectively, were isolated from a cDNA library derived from mRNA extracted from the anaerobic fungus, Piromyces [7].
  • On the basis of conserved protein secondary structure, we describe here the identification and experimental characterization of novel type of mannanase-associated mannan-binding module and also characterization of two CBM family 4 laminarinase-associated beta-glucan-binding modules [8].
  • Both of the CBDs exhibited sequence similarity to family IIIb CBDs, while the mannanase catalytic domain exhibited homology to the family 5 glycosyl hydrolases [9].

Biological context of MANBA

  • These data demonstrate that, like other lysosomal enzyme genes such as those for beta-glucuronidase or beta-hexosaminidase, the human MANB gene is controlled by a short 5' flanking sequence located near the initiation codon [10].
  • The human MANB gene spans approximately 22 kb and consists of 24 exons [10].
  • Different mannanase preparations obtained from the filamentous fungus Sclerotium rolfsii were used for the hydrolysis of coffee mannan, thus reducing significantly the viscosity of coffee extracts [11].
  • Using this method, the DsRed fluorescence protein and mannanase genes have been cloned in the baculovirus genome and expressed in the Sf9 cells [12].

Anatomical context of MANBA

  • Promoter analysis using deletion constructs of the 5' flanking region fused to the bacterial CAT gene showed that 150 bp of 5' sequence could drive the expression of MANB in COS 7 cells [10].

Associations of MANBA with chemical compounds

  • Previously, we found that none of the galactomannan-degrading enzymes were extracellular, and we characterized an outer membrane mannanase which hydrolyzes the backbone of guar gum to produce large fragments [2].
  • Enhanced mannanase production only commenced when glucose was depleted in the medium [13].
  • A total of 31 strains were tested for alpha-amylase, alpha-glucosidase and glucoamylase, beta-glucosidase, lichenase, cellulase, xylanase and xylosidase, mannanase and mannosidase production during growth of microorganisms on respective meshed polysaccharide gels [14].

Other interactions of MANBA


Analytical, diagnostic and therapeutic context of MANBA

  • Using ion-exchange chromatography, gel filtration, and chromatofocusing steps, we have purified the soluble mannanase to apparent homogeneity [2].
  • Together with a specific primer previously derived from the N-terminus, 95.7% of the mannanase gene sequence was obtained from genomic T. fusca DNA by PCR [16].


  1. X4 modules represent a new family of carbohydrate-binding modules that display novel properties. Bolam, D.N., Xie, H., Pell, G., Hogg, D., Galbraith, G., Henrissat, B., Gilbert, H.J. J. Biol. Chem. (2004) [Pubmed]
  2. Purification and characterization of a cell-associated, soluble mannanase from Bacteroides ovatus. Gherardini, F.C., Salyers, A.A. J. Bacteriol. (1987) [Pubmed]
  3. Sequence of the gene for a high-alkaline mannanase from an alkaliphilic Bacillus sp. strain JAMB-750, its expression in Bacillus subtilis and characterization of the recombinant enzyme. Hatada, Y., Takeda, N., Hirasawa, K., Ohta, Y., Usami, R., Yoshida, Y., Grant, W.D., Ito, S., Horikoshi, K. Extremophiles (2005) [Pubmed]
  4. Tumour necrosis factor-alpha production in human alveolar macrophages: modulation by inhaled corticosteroid. Marshall, B.G., Wangoo, A., Harrison, L.I., Young, D.B., Shaw, R.J. Eur. Respir. J. (2000) [Pubmed]
  5. Morphological and biochemical studies of human beta-mannosidosis: identification of a novel beta-mannosidase gene mutation. Uchino, Y., Fukushige, T., Yotsumoto, S., Hashiguchi, T., Taguchi, H., Suzuki, N., Konohana, I., Kanzaki, T. Br. J. Dermatol. (2003) [Pubmed]
  6. Mannosidosis: assignment of the lysosomal alpha-mannosidase B gene to chromosome 19 in man. Champion, M.J., Shows, T.B. Proc. Natl. Acad. Sci. U.S.A. (1977) [Pubmed]
  7. The conserved noncatalytic 40-residue sequence in cellulases and hemicellulases from anaerobic fungi functions as a protein docking domain. Fanutti, C., Ponyi, T., Black, G.W., Hazlewood, G.P., Gilbert, H.J. J. Biol. Chem. (1995) [Pubmed]
  8. Identification of novel beta-mannan- and beta-glucan-binding modules: evidence for a superfamily of carbohydrate-binding modules. Sunna, A., Gibbs, M.D., Bergquist, P.L. Biochem. J. (2001) [Pubmed]
  9. A gene encoding a novel multidomain beta-1,4-mannanase from Caldibacillus cellulovorans and action of the recombinant enzyme on kraft pulp. Sunna, A., Gibbs, M.D., Chin, C.W., Nelson, P.J., Bergquist, P.L. Appl. Environ. Microbiol. (2000) [Pubmed]
  10. Characterization of the human MANB gene encoding lysosomal alpha-D-mannosidase. Wakamatsu, N., Gotoda, Y., Saito, S., Kawai, H. Gene (1997) [Pubmed]
  11. Hydrolysis of isolated coffee mannan and coffee extract by mannanases of Sclerotium rolfsii. Sachslehner, A., Foidl, G., Foidl, N., Gübitz, G., Haltrich, D. J. Biotechnol. (2000) [Pubmed]
  12. Directional and direct cloning strategy for high-throughput generation of recombinant baculoviruses. Ma, Q., Zhou, L., Ma, L., Huo, K. BioTechniques (2006) [Pubmed]
  13. Efficient production of mannan-degrading enzymes by the basidiomycete Sclerotium rolfsii. Sachslehner, A., Haltrich, D., Gübitz, G., Nidetzky, B., Kulbe, K.D. Appl. Biochem. Biotechnol. (1998) [Pubmed]
  14. Polysaccharide hydrolases of Aureobasidium pullulans. Augustín, J. Folia Microbiol. (Praha) (2000) [Pubmed]
  15. Enzymatic activity of endophytic fungi of six native seedling species from Doi Suthep-Pui National Park, Thailand. Lumyong, S., Lumyong, P., McKenzie, E.H., Hyde, K.D. Can. J. Microbiol. (2002) [Pubmed]
  16. Structure elucidation of beta-mannanase: from the electron-density map to the DNA sequence. Hilge, M., Perrakis, A., Abrahams, J.P., Winterhalter, K., Piontek, K., Gloor, S.M. Acta Crystallogr. D Biol. Crystallogr. (2001) [Pubmed]
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