Disease relevance of TAF6

• RNA-stimulated NTPase activity associated with the p80 protein of the pestivirus bovine viral diarrhea virus [1].
• The p80 gene sequence was also integrated into a baculovirus genome for its expression in Spodoptera frugiperda insect cells [2].
• The National Animal Disease Laboratory (NADL) vaccine strain of bovine viral diarrhea virus (BVDV) genes for gp48 and p80 were expressed in Escherichia coli [3].
• A panel of monoclonal antibodies (mAbs) has been produced to the p125/p80 non-structural polypeptide of border disease virus (BDV) and bovine virus diarrhoea virus (BVDV) [4].
• It is apparent, however, that cytopathic bovine viral diarrhea viruses arise by mutation of noncytopathic viruses, and it is known that p80 is the marker protein for cytopathic viruses [5].

High impact information on TAF6

• A serine protease residing in the nonstructural (NS) protein NS3 (p80) has been shown to be essential for the release of the NS proteins located downstream of NS3 [6].
• Expression experiments demonstrated that p80, the marker protein of cytopathogenic BVDV, is translated from the defective genome [7].
• Identification of effector binding sites on S100 beta: studies with guanylate cyclase and p80, a retinal phosphoprotein [8].
• The protein was recently found to activate a retinal membrane guanylate cyclase, and in this paper, we report that it inhibits the phosphorylation of an 80 kDa retinal protein (p80) [8].
• Significant biotype-specific differences were also observed in the lymphocyte proliferative responses of cattle following in vitro stimulation by non-cytopathic/cytopathic BVDV and the non-structural p80 protein (NS3) [9].

Chemical compound and disease context of TAF6

• Based on the presence of specific amino acid sequence motifs, pestivirus nonstructural protein p80 was predicted to be both a serine-type proteinase and a nucleoside triphosphatase (NTPase)/RNA helicase [1].

Biological context of TAF6

• Comparison of the DNA sequence in the p80 region revealed greater homology within the "pairs" than to NADL, which lend further support to the hypothesis that a cp virus is originated from a ncp virus [10].
• The most conserved nucleotide and amino acid sequences between BVDV2-890 and other pestiviruses are located in the region coding for the nonstructural protein p80 [11].
• Sixteen cytopathogenic (cp) bovine viral diarrhea virus (BVDV) strains/isolates were screened for the existence of RNA insertions in the p125 gene region and/or for p80 gene duplications using the polymerase chain reaction after reverse transcription [12].
• Finally, the potential role of p80 proteinase activity in the phenotype of cytopathic biotypes of BVDV is discussed [13].
• We previously demonstrated p80 possesses the former activity (Wisherchen and Collett, Virology 184, 341-350, 1991) [1].

Anatomical context of TAF6

• Detection of bovine viral diarrhoea virus p80 protein in subpopulations of bovine leukocytes [14].

Associations of TAF6 with chemical compounds

• By determining the effects of including or excluding dithiothreitol in the assays, we observed that the cysteine residue in the C-terminal region of S100 beta (Cys84) participates in the regulation of guanylate cyclase but not of p80 phosphorylation [8].
• Bovine viral diarrhea virus proteins: relatedness of p175 with p80 and p125 and evidence of glycoprotein processing [15].

Analytical, diagnostic and therapeutic context of TAF6

• We have used four pairs of cp/ncp BVDV isolated from cattle with mucosal disease, to examine the genomic sequence of the region of the genome coding for the nonstructural protein p125 (processed to p54/p80 in cp viruses) by PCR analysis and sequencing [10].
• Expression of the bovine viral diarrhoea virus Osloss p80 protein: its use as ELISA antigen for cattle serum antibody detection [2].
• BVDV2-890 is noncytopathic in cell culture and does not produce a p80 viral polypeptide [11].
• Rabbit antisera were raised against this p80 recombinant antigen and assayed for the immunoprecipitation of either p120 or p80 protein from cytopathic or non-cytopathic BVDV biotype-infected bovine cells [2].
• In addition, p80 gene duplications in the genomes of the cpBVDV clones were analysed using the polymerase chain reaction and subsequent restriction enzyme analysis of the amplicons [16].

References