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Gene Review

RBP1  -  retinol binding protein 1, cellular

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Disease relevance of RBP1

  • RalDH(II) expressed in E. coli recognizes as substrates free retinal, with a Km of approximately 0.7 microM, and cellular retinol-binding protein-bound retinal, with a Km of approximately 0.2 microM [1].

High impact information on RBP1

  • RalDH(II) also can utilize as substrate retinal generated in situ by microsomal retinol dehydrogenases, from the physiologically most abundant substrate: retinol bound to cellular retinol-binding protein [1].
  • ApoCRBP proved to be a specific inhibitor of retinoic acid synthesis from CRBP-bound retinol [2].
  • NAD-supported reactions carried out in the presence or in the absence of dimethyl sulfoxide yielded apparent Km and Vmax values for the retinol-CRBP complex of 3.5 +/- 0.6 microM, 611 +/- 49 pmol h-1 (mg of protein)-1, and 0.84 +/- 0.12 microM, 601 +/- 38 pmol h-1 (mg of protein)-1, respectively [2].
  • Cytosolic retinoic acid synthesis from retinol-CRBP was strictly dependent on the exogenous supply of either NAD or NADP [2].
  • Reduction of retinaldehyde bound to cellular retinol-binding protein (type II) by microsomes from rat small intestine [3].

Biological context of RBP1


Anatomical context of RBP1


Associations of RBP1 with chemical compounds

  • Cellular retinol-binding protein (type II) (CRBP(II)), a newly described retinol-binding protein, is present in the small intestinal absorptive cell at high levels [9].
  • Mobilization of retinol stored as a membrane-bound retinyl-ester is mediated by a membrane-associated hydrolase activity selectively controlled by the level of apo-CRBP which acts as a carrier for the released retinol [4].
  • Both the induction and degradation of CRBP mRNA were inhibited by the protein synthesis inhibitor cycloheximide [7].
  • Mass spectrometric (MS) analysis of isotopically labeled retinoids showed that isomerization proceeds via alkyl cleavage mechanism, but the product of isomerization depended on the specificity of the retinoid-binding protein(s) as evidenced by the production of 13-cis-retinol in the presence of cellular retinoid-binding protein (CRBP) [11].
  • The results of these and previous studies indicate that CRBP is particularly sensitive to modifications of the retinol hydroxyl end group [12].

Physical interactions of RBP1


Other interactions of RBP1


Analytical, diagnostic and therapeutic context of RBP1

  • To complement the absorbance measurements, the IRBP and CRBP in these mixtures were subsequently separated by size-exclusion HPLC and individually analysed for retinol content by scanning the effluent with a multiple-diode-array detector [15].
  • Northern blot analysis demonstrated that thecal and granulosa cells from antral follicles and luteal tissue expressed RBP and CRBP mRNA [10].
  • The interactions with retinol and retinol analogs of bovine cellular retinol-binding protein (CRBP) have been investigated, by means of fluorescence titrations, to obtain more information on the structural features of retinoid that may be required for their interaction with the binding protein [12].


  1. Cloning of a cDNA encoding an aldehyde dehydrogenase and its expression in Escherichia coli. Recognition of retinal as substrate. Wang, X., Penzes, P., Napoli, J.L. J. Biol. Chem. (1996) [Pubmed]
  2. Retinol bound to cellular retinol-binding protein is a substrate for cytosolic retinoic acid synthesis. Ottonello, S., Scita, G., Mantovani, G., Cavazzini, D., Rossi, G.L. J. Biol. Chem. (1993) [Pubmed]
  3. Reduction of retinaldehyde bound to cellular retinol-binding protein (type II) by microsomes from rat small intestine. Kakkad, B.P., Ong, D.E. J. Biol. Chem. (1988) [Pubmed]
  4. Vitamin A uptake from retinol-binding protein in a cell-free system from pigment epithelial cells of bovine retina. Retinol transfer from plasma retinol-binding protein to cytoplasmic retinol-binding protein with retinyl-ester formation as the intermediate step. Ottonello, S., Petrucco, S., Maraini, G. J. Biol. Chem. (1987) [Pubmed]
  5. Identification of the endogenous retinoids associated with three cellular retinoid-binding proteins from bovine retina and retinal pigment epithelium. Saari, J.C., Bredberg, L., Garwin, G.G. J. Biol. Chem. (1982) [Pubmed]
  6. The primary structure of bovine cellular retinoic acid-binding protein. Sundelin, J., Das, S.R., Eriksson, U., Rask, L., Peterson, P.A. J. Biol. Chem. (1985) [Pubmed]
  7. Serum factors induce messenger ribonucleic acid levels for cellular retinol-binding protein in rat Sertoli cells. Kroepelien, C.F., Knutsen, H.K., Haugen, T.B., Hansson, V., Eskild, W. Endocrinology (1993) [Pubmed]
  8. Peroxidation stimulated by lipid hydroperoxides on bovine retinal pigment epithelium mitochondria: effect of cellular retinol-binding protein. Terrasa, A.M., Guajardo, M.H., Catalá, A. Int. J. Biochem. Cell Biol. (2003) [Pubmed]
  9. Acyl-CoA-independent esterification of retinol bound to cellular retinol-binding protein (type II) by microsomes from rat small intestine. Ong, D.E., Kakkad, B., MacDonald, P.N. J. Biol. Chem. (1987) [Pubmed]
  10. Expression of retinol-binding protein and cellular retinol-binding protein in the bovine ovary. Brown, J.A., Eberhardt, D.M., Schrick, F.N., Roberts, M.P., Godkin, J.D. Mol. Reprod. Dev. (2003) [Pubmed]
  11. Isomerization of all-trans-retinol to cis-retinols in bovine retinal pigment epithelial cells: dependence on the specificity of retinoid-binding proteins. McBee, J.K., Kuksa, V., Alvarez, R., de Lera, A.R., Prezhdo, O., Haeseleer, F., Sokal, I., Palczewski, K. Biochemistry (2000) [Pubmed]
  12. Interactions with retinol and retinoids of bovine cellular retinol-binding protein. Malpeli, G., Stoppini, M., Zapponi, M.C., Folli, C., Berni, R. Eur. J. Biochem. (1995) [Pubmed]
  13. Properties and immunocytochemical localization of three retinoid-binding proteins from bovine retina. Saari, J.C., Bunt-Milam, A.H., Bredberg, D.L., Garwin, G.G. Vision Res. (1984) [Pubmed]
  14. Isolation and characterization of an unsaturated fatty acid-binding protein from developing chick neural retina. Lee, L., Wiggert, B. J. Neurochem. (1984) [Pubmed]
  15. Exchange of retinol between IRBP and CRBP. Edwards, R.B., Adler, A.J. Exp. Eye Res. (1994) [Pubmed]
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