Disease relevance of CENPK

• In transient transfection assays in CHO cells with SoxLZ/Sox6 containing the transactivational region of herpes simplex virus VP16, expression of a reporter gene that carries a cis binding region for Sox proteins was significantly enhanced by the co-expression of Solt and Ca(2+)/calmodulin-dependent protein kinase IV [1].
• A novel interaction involving a polypeptide chain (P33) in covalent linkage with IgM on the surface of a Burkitt lymphoma cell line (Daudi) [2].
• The linkage of P33 to surface immunoglobulin has not been observed with any other human lymphoma or lymphoblastoid cell lines [2].
• After 3 rounds of biopanning, the positive phages were confirmed by competitive ELISA using HBsAg/P33 [3].
• These results indicate that P33 is an active component of SILK, conferring protection against Salmonella Enteritidis by augmenting the antimicrobial activities of heterophils [4].

High impact information on CENPK

• In a previous paper (Barboro et al., 1993, Biophys. J. 65, 1690-1699) we have shown that cancer development in the resistant hepatocyte model of Solt and Farber is characterized by the progressive unfolding of the higher-order structure of chromatin [5].
• The results presented here provide direct evidence that one or more of the three proteins that have cross-linked to the P33 are responsible for binding the manganese of the oxygen-evolving complex [6].
• The extrinsic 33-kDa protein (P33) was cross-linked covalently to the binding site on P33-depleted PSII preparations which is responsible for reconstitution of photosynthetic water oxidation after PSII preparations have been washed with 1 M CaCl2 [6].
• Since P33 and kappa chains cosegregate in all primary hybrid clones and subclones, it is concluded that they are coded for by the same human chromosome 2 [7].
• This novel polypeptide chain (P33) is similar in several properties to the heavy chain of the Ia antigen: glycoprotein nature; apparent molecular weight; isoelectric behavior and cysteine content [2].

Anatomical context of CENPK

• We have expressed mim-1 in Escherichia coli and demonstrated in vitro that recombinant P33 is chemotactic for heterophils, the avian equivalent of mammalian neutrophils [8].
• P33 protein was isolated from the cell walls of Candida utilis [9].
• In normal subjects N21 was consistently recorded over L1 vertebra and in most subjects a complex, N27/N29/P33, was recorded over the cervical spine referred to the vertex [10].

Associations of CENPK with chemical compounds

• Because the liver is a major site for bioactivation of Tris-BP to 2BA in vivo, we tested the initiating capacity of Tris-BP in the rat liver in a modified Solt & Farber initiation and promotion system [11].
• In situ hybridization with a P33-labelled cDNA probe was used to analyze the effects of castration and replacement therapy by testosterone on the number of neurons expressing vasotocin mRNA in the male quail brain [12].
• Eleven patients were paced in VAT mode (Cordis 208A or Siemens-Elema 625 generator), two patients in DVI mode (Intermedics Cyberlith IV generator) and four patients in DDD mode (Siemens-Elema 664/P33 or Telectronics Autima unit) [13].

References