Disease relevance of SLC25A1

• Expression of Na+-dependent citrate transport in a strongly metastatic human prostate cancer PC-3M cell line: regulation by voltage-gated Na+ channel activity [1].
• The human mitochondrial citrate transporter gene (SLC20A3) maps to chromosome band 22q11 within a region implicated in DiGeorge syndrome, velo-cardio-facial syndrome and schizophrenia [2].

High impact information on SLC25A1

• Although fatty acid synthesis in adipose tissue or the liver is initiated by citrate transport in exchange for malate across the mitochondrial membrane, the transporter responsible for supplying malate during citrate transport has not been identified [3].
• A detailed comparison of citrate uptake into the vacuole-like lutoids of rubber tree (Hevea brasiliensis Muell. Arg.) and of malate and citrate transport into barley (Hordeum vulgare L.) vacuoles revealed very similar transport specificities [4].
• In the present study, we investigated the functional characteristics of Na+-dependent succinate and citrate transport in primary cultures of astrocytes and neurons from rat cerebral cortex [5].
• In our previous study, we characterized electrophysiologically the mechanism of citrate transport in a normal prostatic epithelial (PNT2-C2) cell line [1].
• Using this methodology, we have mapped the human homolog of a rodent citrate transport protein to the DGCR [6].

Biological context of SLC25A1

• The gene encoding the human mitochondrial citrate transporter designated SLC20A3 was mapped to chromosome 22 by analyzing its segregation in a panel of human-hamster somatic cell hybrids [2].
• Cells transfected with the plasmid pSV-201, containing the cDNA for the Na+/dicarboxylate cotransporter, expressed sodium-dependent succinate and citrate transport after 48 h [7].

Anatomical context of SLC25A1

• In the present study, we investigated citrate transport in a normal human prostate cell line, PNT2-C2, using mainly electrophysiological methods [8].
• Characterization of citrate transport through the plasma membrane in a carrot mutant cell line with enhanced citrate excretion [9].
• Citrate transport in liposomes reconstituted with triton extracts from mitochondria [10].
• The effect of agaric acid on citrate transport in rat liver mitochondria [11].

Associations of SLC25A1 with chemical compounds

• In primary cultures of neurons, uptake of citrate was also Na+ dependent and saturable with a Kt value of 16.2 microM, which was different from that observed in astrocytes, suggesting that different Na+-dependent citrate transport systems are expressed in neurons and astrocytes [5].
• The mitochondrial citrate transport protein: Evidence for a steric interaction between glutamine 182 and leucine 120 and its relationship to the substrate translocation pathway and identification of other mechanistically essential residues [12].

Other interactions of SLC25A1

• Cloning, genomic organization, and chromosomal localization of human citrate transport protein to the DiGeorge/velocardiofacial syndrome minimal critical region [6].

References