The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Nucb1  -  nucleobindin 1

Rattus norvegicus

Synonyms: Bone 63 kDa calcium-binding protein, CALNUC, Nuc, Nucleobindin-1
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Psychiatry related information on Nucb1

  • CONCLUSIONS: Our results suggest that the modulatory effect of OFQ on locomotor activity is not solely due to its inhibitory action on extracellular DA in the Nuc Acc [1].

High impact information on Nucb1

  • Calnuc, an EF-hand Ca(2+) binding protein, specifically interacts with the C-terminal alpha5-helix of G(alpha)i3 [2].
  • Peptide competition indicated that calnuc, with its coiled-coil domain constituted by the two EF-hands, binds to G(alpha)i3's C-terminal alpha5-helix [2].
  • By yeast two-hybrid analysis, the binding site on G(alpha)i3 for calnuc was mapped to the C-terminal region because removal of the last 12 amino acids (but not 11) abolished the interaction [2].
  • That calnuc interacts with G(alpha)i3 in vivo was verified by the finding that G(alpha)i3 could be crosslinked intracellularly to calnuc and co-immunoprecipitated from NIH 3T3 cells stably overexpressing either activated (Q204L) or inactivated (G203A) G(alpha)i3 [2].
  • In pulse-chase experiments, the two pools of calnuc had different mobilities, suggesting different posttranslational modifications [2].

Chemical compound and disease context of Nucb1


Biological context of Nucb1

  • Semiquantitative RT-PCR and Northern blot analysis showed Nuc expression to be low during cell proliferation, upregulated during differentiation and matrix maturation, but subsequently downregulated during mineralization [3].
  • Finally, the tissue distribution patterns of Nuc labeling were compared in non-retrieved HPF-FS sections to that of retrieved and non-retrieved PFF sections [4].

Anatomical context of Nucb1


Associations of Nucb1 with chemical compounds

  • OFQ (30 nmol, i.c.v.) also attenuated both the basal and the cocaine-induced increase in extracellular DA in the Nuc Acc. OFQ, at the highest dose, also decreased the motor stimulation induced by apomorphine [1].
  • In comparison to controls we observed an increase in the density of VILIP-1 immunoreactive (IR) hippocampal interneurons and presubicular nerve cells in ketamine treated rats, whereas the density of VILIP-1 expressing cells was decreased in the Nuc. accumbens of these rats [7].
  • In consideration of the known functional properties of nucleobindin, it may be postulated that nucleobindin may contribute to the accumulation and transport of Ca2+ ions to the mineralization front prior to hydroxyapatite deposition [6].
  • In addition, the existence in C62B cells of a cyclic AMP-induced, cycloheximide-sensitive desensitization process, such as that reported by others (Terasaki, et al. Adv. Cyclic Nuc. Res. 9: 33, 1978) is confirmed [8].

Other interactions of Nucb1

  • The presence of a signal sequence in Nuc suggests secretion from the cell and it has been found in bone extracellular matrix [3].
  • Compared to bone sialoprotein and osteopontin, Nuc demonstrated different localization pattern in bone trabeculae, with the majority of labeling restricted to nonmineralized osteoid [3].

Analytical, diagnostic and therapeutic context of Nucb1

  • By electron microscopy, immunolabeling for Nuc was seen in osteoid of newly formed bone, on all surfaces facing the various bone cells and also in compact bone [3].
  • Western blot analysis of tooth protein extracts demonstrated the presence of a 63 kDa protein, which showed immunologic affinity for a rat nucleobindin peptide antibody [6].
  • Nucleobindin mRNA transcripts were detected in the tooth, and in situ hybridization analysis substantiated the findings, showing nucleobindin expression within mature odontoblasts and within the cells of surrounding developing alveolar bone [6].
  • As an additional experiment, the effect of antigen retrieval (for Nuc) in sections of tissue primary stabilized by high pressure freezing with subsequent freeze substitution (HPF-FS) was studied [4].


  1. Orphanin FQ/nociceptin attenuates motor stimulation and changes in nucleus accumbens extracellular dopamine induced by cocaine in rats. Lutfy, K., Do, T., Maidment, N.T. Psychopharmacology (Berl.) (2001) [Pubmed]
  2. Calnuc, an EF-hand Ca(2+) binding protein, specifically interacts with the C-terminal alpha5-helix of G(alpha)i3. Lin, P., Fischer, T., Weiss, T., Farquhar, M.G. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  3. Nucleobindin is produced by bone cells and secreted into the osteoid, with a potential role as a modulator of matrix maturation. Petersson, U., Somogyi, E., Reinholt, F.P., Karlsson, T., Sugars, R.V., Wendel, M. Bone (2004) [Pubmed]
  4. Heat-induced retrieval of immunogold labeling for nucleobindin and osteoadherin from Lowicryl sections of bone. Solberg, L.B., Melhus, G., Brorson, S.H., Wendel, M., Reinholt, F.P. Micron (2006) [Pubmed]
  5. Characterization of Cos-7 cells overexpressing the rat secretory pathway Ca2+-ATPase. Reinhardt, T.A., Horst, R.L., Waters, W.R. Am. J. Physiol., Cell Physiol. (2004) [Pubmed]
  6. Nucleobindin--a Ca2+-binding protein present in the cells and mineralized tissues of the tooth. Somogyi, E., Petersson, U., Sugars, R.V., Hultenby, K., Wendel, M. Calcif. Tissue Int. (2004) [Pubmed]
  7. Brain region-specific changes in the expression of calcium sensor proteins after repeated applications of ketamine to rats. Bernstein, H.G., Becker, A., Keilhoff, G., Spilker, C., Gorczyca, W.A., Braunewell, K.H., Grecksch, G. Neurosci. Lett. (2003) [Pubmed]
  8. Characterization of agonist-induced beta-adrenergic receptor-specific desensitization in C62B glioma cells. Frederich, R.C., Waldo, G.L., Harden, T.K., Perkins, J.P. Journal of cyclic nucleotide and protein phosphorylation research. (1983) [Pubmed]
WikiGenes - Universities