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Gene Review

NUCB1  -  nucleobindin 1

Homo sapiens

Synonyms: CALNUC, Calnuc, NUC, Nucleobindin-1
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Disease relevance of NUCB1

  • Upregulation of nucleobindin expression in human-activated lymphocytes and non-Hodgkin's lymphoma [1].
  • The percentages of patients with plasma HIV RNA < or = 50 copies/ml at 48 weeks by drug class were: PI triple, 46% (95% CI, 41 to 52%); NNRTI triple, 51% (95% CI, 43 to 59%); triple NUC, 45% (95% CI, 36 to 54%) [2].
  • However, the frequency of autoantibody to Calnuc in colon cancer (11.5%) was significantly higher than that in normal individuals (1.2%) [3].
  • In tumors with lymph node metastasis, 41 (82%) were positive for u-PA and 28 (56%) were positive for Nuc [4].
  • These results suggest that immunohistochemical examination for the expression of u-PA and Nuc in tumor cells may help evaluate the potential of adenocarcinomas of the stomach for lymph node metastasis [4].

High impact information on NUCB1


Biological context of NUCB1


Anatomical context of NUCB1

  • These results indicate that cytosolic calnuc binds to Galphai3 on Golgi membranes in living cells and that Galphai3 must be anchored to the cytosolic surface of Golgi membranes via lipid anchors for the interaction to occur [7].
  • To investigate whether Galphai3 binds calnuc in living cells and where this interaction takes place we performed fluorescence resonance energy transfer (FRET) analysis between Galphai3 and calnuc in COS-7 cells expressing Galphai3-yellow fluorescent protein (YFP) and calnuc-cyan fluorescent protein (CFP) [7].
  • Expression of Nuc was first studied in human lymphocytes [1].
  • The numerical density Nv (NUC/EPI) of tumor cell nuclei turned out to be a most reliable morphological marker of the concordant ER and PR status (overall efficiency = 84%, p < 0.01) [10].

Associations of NUCB1 with chemical compounds

  • Calnuc has the properties of a Ca(2+) sensor protein capable of binding to and potentially regulating interactions of Galphai3 on Golgi membranes [7].
  • The TATA sequence of the human, estrogen-responsive pS2 promoter is complexed in vivo with a rotationally and translationally positioned nucleosome (NUC T) [11].
  • The treatment of antiretroviral-naive subjects with the 3TC/zidovudine combination: a review of North American (NUCA 3001) and European (NUCB 3001) trials [12].
  • Spectral deconvolution of TRLFS measurements on the NUC revealed the unexpected presence of two distinct chemical environments consistent with published spectra of U(VI)-substituted synthetic calcite and aragonite [13].
  • Calnuc is a calcium (Ca2+) binding protein found in both Golgi and cytoplasm, and it may play a role in G protein- and Ca2+-regulated signal transduction events [3].

Other interactions of NUCB1


Analytical, diagnostic and therapeutic context of NUCB1

  • By immunoelectron microscopy, CALNUC is localized to cis-Golgi cisternae and the cis-Golgi network (CGN) [6].
  • Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure [15].
  • METHODS: A systematic overview of results from clinical trials involving triple combination therapy with dual nucleoside reverse transcriptase inhibitors (NRTI) and: a protease inhibitor (PI triple); a non-nucleoside reverse transcriptase inhibitor (NNRTI triple); or a third NRTI (triple NUC) [2].
  • Analysis by circular dichroism spectrometry indicated that addition of Ca2+ ions induces a structural change in Nuc, presumably an increase in the amount of alpha helix [16].
  • Purified full-length recombinant Calnuc protein was used as an antigen in enzyme-linked immunoassay and Western blotting for the detection of autoantibodies in cancers [3].


  1. Upregulation of nucleobindin expression in human-activated lymphocytes and non-Hodgkin's lymphoma. Kubota, T., Miyauchi, M., Miura, K., Hirokawa, G., Awaya, A., Miyasaka, N., Kurosawa, Y., Kanai, Y., Maruyama, K. Pathol. Int. (1998) [Pubmed]
  2. Overview of the effectiveness of triple combination therapy in antiretroviral-naive HIV-1 infected adults. Bartlett, J.A., DeMasi, R., Quinn, J., Moxham, C., Rousseau, F. AIDS (2001) [Pubmed]
  3. Autoantibodies to Ca2+ binding protein Calnuc is a potential marker in colon cancer detection. Chen, Y., Lin, P., Qiu, S., Peng, X.X., Looi, K., Farquhar, M.G., Zhang, J.Y. Int. J. Oncol. (2007) [Pubmed]
  4. Antigen expression associated with lymph node metastasis in gastric adenocarcinomas. Wang, S.N., Miyauchi, M., Koshikawa, N., Maruyama, K., Kubota, T., Miura, K., Kurosawa, Y., Awaya, A., Kanai, Y. Pathol. Int. (1994) [Pubmed]
  5. Overexpression of CALNUC (nucleobindin) increases agonist and thapsigargin releasable Ca2+ storage in the Golgi. Lin, P., Yao, Y., Hofmeister, R., Tsien, R.Y., Farquhar, M.G. J. Cell Biol. (1999) [Pubmed]
  6. The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein. Lin, P., Le-Niculescu, H., Hofmeister, R., McCaffery, J.M., Jin, M., Hennemann, H., McQuistan, T., De Vries, L., Farquhar, M.G. J. Cell Biol. (1998) [Pubmed]
  7. Galpha i3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins. Weiss, T.S., Chamberlain, C.E., Takeda, T., Lin, P., Hahn, K.M., Farquhar, M.G. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  8. Interaction of the protein nucleobindin with G alpha i2, as revealed by the yeast two-hybrid system. Mochizuki, N., Hibi, M., Kanai, Y., Insel, P.A. FEBS Lett. (1995) [Pubmed]
  9. Organization of the human gene for nucleobindin (NUC) and its chromosomal assignment to 19q13.2-q13.4. Miura, K., Hirai, M., Kanai, Y., Kurosawa, Y. Genomics (1996) [Pubmed]
  10. Stereologic parameters of nuclear ultrastructure as markers of the steroid receptor status in breast cancer epithelium. Savjak, D., Pikula, B., Muss, W.H., Hacker, G.W., Amidzic, L., Hauser-Kronberger, C., Lelic-Marinovic, M., Dietze, O. Pathol. Res. Pract. (1994) [Pubmed]
  11. Binding of TATA binding protein to a naturally positioned nucleosome is facilitated by histone acetylation. Sewack, G.F., Ellis, T.W., Hansen, U. Mol. Cell. Biol. (2001) [Pubmed]
  12. The treatment of antiretroviral-naive subjects with the 3TC/zidovudine combination: a review of North American (NUCA 3001) and European (NUCB 3001) trials. Eron, J.J. AIDS (1996) [Pubmed]
  13. Cryogenic laser induced U(VI) fluorescence studies of a U(VI) substituted natural calcite: implications to U(VI) speciation in contaminated Hanford sediments. Wang, Z., Zachara, J.M., Mckinley, J.P., Smith, S.C. Environ. Sci. Technol. (2005) [Pubmed]
  14. Gene expression profile of serial samples of transformed B-cell lymphomas. de Vos, S., Hofmann, W.K., Grogan, T.M., Krug, U., Schrage, M., Miller, T.P., Braun, J.G., Wachsman, W., Koeffler, H.P., Said, J.W. Lab. Invest. (2003) [Pubmed]
  15. Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure. Miura, K., Titani, K., Kurosawa, Y., Kanai, Y. Biochem. Biophys. Res. Commun. (1992) [Pubmed]
  16. Calcium-binding activity of nucleobindin mediated by an EF hand moiety. Miura, K., Kurosawa, Y., Kanai, Y. Biochem. Biophys. Res. Commun. (1994) [Pubmed]
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