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MSI1  -  Msi1p

Saccharomyces cerevisiae S288c

Synonyms: CAC3, CAF-1 p50 subunit, Chromatin assembly factor 1 subunit p50, IRA1 multicopy suppressor, Protein MSI1, ...
 
 
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High impact information on MSI1

  • RbAp48 share sequence homology with MSI1, a negative regulator of the Ras-cyclic AMP pathway in the yeast Saccharomyces cerevisiae [1].
  • Loss of the other CAF-I subunits (Cac2p and Cac3p/Msi1p) also results in the shmoo cluster phenotype, implying that loss of CAF-I activity gives rise to this unstable repression of HML [2].
  • MSI1 is a multicopy suppressor of a mutation in the IRA1 gene involved in the Ras-cAMP pathway that regulates cellular growth [3].
  • Overexpression of the MSI1 gene could suppress the heat shock sensitivity and the defect in sporulation caused by the ira1 and RAS2Val19 mutations but not those of the bcy1 mutation [4].
  • One such suppressor, MSI1, was found to encode a putative protein of 422 amino acids that shows homology to the beta subunit of the mammalian guanine nucleotide-binding regulatory proteins [4].
 

Biological context of MSI1

 

Associations of MSI1 with chemical compounds

 

Physical interactions of MSI1

  • In a screen for silencing-impaired cac1 alleles, we isolated a mutation that reduced binding to the Cac3p subunit and another that impaired binding to the DNA replication protein PCNA [9].
 

Other interactions of MSI1

  • Deletion of NPR1, like overexpression of Cac3p, suppressed the RAS/cAMP pathway [6].
  • Among the nine genes recovered were two genes from the cAMP-dependent protein kinase (cAPK) pathway, MSI1 and PDE2 [10].
  • The double cac3 hir3 mutation also caused the production of a short transcript from the MET3-URA3 fusion under both repressed and derepressed conditions [11].
  • Saccharomyces cerevisiae mutants lacking CAF-I subunit genes (CAC1 to CAC3) display reduced heterochromatic gene silencing [9].
  • The fortuitous presence of a mutation in the CAC3 gene in the strain in which this screen was undertaken enabled us to discover that double mutaions of cac3 and hir3, but neither of the two single mutations, caused a dramatic increase in the rate of retrotransposition [8].

References

  1. A retinoblastoma-binding protein related to a negative regulator of Ras in yeast. Qian, Y.W., Wang, Y.C., Hollingsworth, R.E., Jones, D., Ling, N., Lee, E.Y. Nature (1993) [Pubmed]
  2. Chromatin assembly factor I contributes to the maintenance, but not the re-establishment, of silencing at the yeast silent mating loci. Enomoto, S., Berman, J. Genes Dev. (1998) [Pubmed]
  3. A conserved family of WD-40 proteins binds to the retinoblastoma protein in both plants and animals. Ach, R.A., Taranto, P., Gruissem, W. Plant Cell (1997) [Pubmed]
  4. MSI1, a negative regulator of the RAS-cAMP pathway in Saccharomyces cerevisiae. Ruggieri, R., Tanaka, K., Nakafuku, M., Kaziro, Y., Toh-e, A., Matsumoto, K. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  5. RIM2, MSI1 and PGI1 are located within an 8 kb segment of Saccharomyces cerevisiae chromosome II, which also contains the putative ribosomal gene L21 and a new putative essential gene with a leucine zipper motif. Démolis, N., Mallet, L., Bussereau, F., Jacquet, M. Yeast (1993) [Pubmed]
  6. CAC3(MSI1) suppression of RAS2(G19V) is independent of chromatin assembly factor I and mediated by NPR1. Johnston, S.D., Enomoto, S., Schneper, L., McClellan, M.C., Twu, F., Montgomery, N.D., Haney, S.A., Broach, J.R., Berman, J. Mol. Cell. Biol. (2001) [Pubmed]
  7. MSI1 suppresses hyperactive RAS via the cAMP-dependent protein kinase and independently of chromatin assembly factor-1. Zhu, X., Démolis, N., Jacquet, M., Michaeli, T. Curr. Genet. (2000) [Pubmed]
  8. Yeast Ty1 retrotransposition is stimulated by a synergistic interaction between mutations in chromatin assembly factor I and histone regulatory proteins. Qian, Z., Huang, H., Hong, J.Y., Burck, C.L., Johnston, S.D., Berman, J., Carol, A., Liebman, S.W. Mol. Cell. Biol. (1998) [Pubmed]
  9. Chromatin assembly factor I mutants defective for PCNA binding require Asf1/Hir proteins for silencing. Krawitz, D.C., Kama, T., Kaufman, P.D. Mol. Cell. Biol. (2002) [Pubmed]
  10. Relationship of the cAMP-dependent protein kinase pathway to the SNF1 protein kinase and invertase expression in Saccharomyces cerevisiae. Hubbard, E.J., Yang, X.L., Carlson, M. Genetics (1992) [Pubmed]
  11. Host genes that affect the target-site distribution of the yeast retrotransposon Ty1. Huang, H., Hong, J.Y., Burck, C.L., Liebman, S.W. Genetics (1999) [Pubmed]
 
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