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Gene Review

ECs1601  -  sensor protein PhoQ

Escherichia coli O157:H7 str. Sakai

 
 
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Disease relevance of ECs1601

  • The Salmonella typhimurium PhoP/PhoQ-activated gene pagP is required both for biosynthesis of hepta-acylated lipid A species containing palmitate and for resistance to cationic anti-microbial peptides [1].
  • In Escherichia coli, low Mg(2+) leads to only modest RpoS stabilization, and iraP is not regulated by PhoP/PhoQ [2].
  • Here, we apply this method to probe the regulatory networks governed by the PhoP/PhoQ two-component system in the enteric bacteria Escherichia coli and Salmonella enterica [3].
  • Comparison of the Pseudomonas aeruginosa and Escherichia coli PhoQ sensor domains: evidence for distinct mechanisms of signal detection [4].
  • One PhoP/PhoQ-activated OMP was identified by semiautomated tandem mass spectrometry coupled with electronic database searching as PgtE, a member of the Escherichia coli OmpT and Yersinia pestis Pla family of outer membrane proteases [5].
 

High impact information on ECs1601

  • This process requires the two-component regulatory system PhoP/PhoQ, which is specifically activated in low Mg(2+) [2].
  • PhoQ is a transmembrane histidine kinase belonging to the family of two-component signal transducing systems common in prokaryotes and lower eukaryotes [6].
  • A PhoP/PhoQ-induced Lipase (PagL) that catalyzes 3-O-deacylation of lipid A precursors in membranes of Salmonella typhimurium [7].
  • Structural and mutational analysis of the PhoQ histidine kinase catalytic domain. Insight into the reaction mechanism [6].
  • We have determined the structure of the kinase domain of Escherichia coli PhoQ complexed with the non-hydrolyzable ATP analog adenosine 5'-(beta,gamma-imino)triphosphate and Mg(2+) [6].
 

Chemical compound and disease context of ECs1601

  • Characterization of the catalytic activities of the PhoQ histidine protein kinase of Salmonella enterica serovar Typhimurium [8].
  • A Salmonella enterica serovar Typhimurium PhoQ sensor kinase mutant, in which the threonine at residue 48 in the periplasmic sensor domain is changed to an isoleucine, was shown previously to result in elevated expression of PhoP-activated genes and to affect mouse virulence, epithelial cell invasion, and sensitivity to macrophage killing [9].
 

Biological context of ECs1601

  • S. typhimurium mutated in the PhoP/PhoQ signal transduction system caused significantly less killing of C. elegans [10].
  • Co-regulation of Salmonella enterica genes required for virulence and resistance to antimicrobial peptides by SlyA and PhoP/PhoQ [11].
  • However, when Mg2) or Mn2+ was present at concentrations higher than 0.1 mM, the kinetics of PhoQ autophosphorylation were strongly biphasic, with a rapid autophosphorylation phase followed by a slower dephosphorylation phase [8].
  • The amino acid sequences of PhoP and PhoQ of E. coli were 93 and 86% identical, respectively, to those of S. typhimurium [12].
  • We characterized a complete set of proteins having amino acid substitutions at position 48 in the closely related Escherichia coli PhoQ protein [9].
 

Associations of ECs1601 with chemical compounds

  • Acetyl coenzyme A inhibited the autokinase activity of PhoQ in vitro, suggesting that the in vivo repressing effect may be due to a direct inhibition mechanism [13].
  • Considering the fact that the PhoQ/P signaling system responds to external magnesium, we obtained evidence which supports the view that there is a signaling network that connects the Rcs system with the PhoQ/P system, which coordinately regulates extracellular polysaccharide synthesis in response to the external concentrations of divalent cations [14].
 

Analytical, diagnostic and therapeutic context of ECs1601

References

  1. Transfer of palmitate from phospholipids to lipid A in outer membranes of gram-negative bacteria. Bishop, R.E., Gibbons, H.S., Guina, T., Trent, M.S., Miller, S.I., Raetz, C.R. EMBO J. (2000) [Pubmed]
  2. The PhoP/PhoQ two-component system stabilizes the alternative sigma factor RpoS in Salmonella enterica. Tu, X., Latifi, T., Bougdour, A., Gottesman, S., Groisman, E.A. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  3. Dissecting the PhoP regulatory network of Escherichia coli and Salmonella enterica. Zwir, I., Shin, D., Kato, A., Nishino, K., Latifi, T., Solomon, F., Hare, J.M., Huang, H., Groisman, E.A. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  4. Comparison of the Pseudomonas aeruginosa and Escherichia coli PhoQ sensor domains: evidence for distinct mechanisms of signal detection. Lesley, J.A., Waldburger, C.D. J. Biol. Chem. (2001) [Pubmed]
  5. A PhoP-regulated outer membrane protease of Salmonella enterica serovar typhimurium promotes resistance to alpha-helical antimicrobial peptides. Guina, T., Yi, E.C., Wang, H., Hackett, M., Miller, S.I. J. Bacteriol. (2000) [Pubmed]
  6. Structural and mutational analysis of the PhoQ histidine kinase catalytic domain. Insight into the reaction mechanism. Marina, A., Mott, C., Auyzenberg, A., Hendrickson, W.A., Waldburger, C.D. J. Biol. Chem. (2001) [Pubmed]
  7. A PhoP/PhoQ-induced Lipase (PagL) that catalyzes 3-O-deacylation of lipid A precursors in membranes of Salmonella typhimurium. Trent, M.S., Pabich, W., Raetz, C.R., Miller, S.I. J. Biol. Chem. (2001) [Pubmed]
  8. Characterization of the catalytic activities of the PhoQ histidine protein kinase of Salmonella enterica serovar Typhimurium. Montagne, M., Martel, A., Le Moual, H. J. Bacteriol. (2001) [Pubmed]
  9. Mutational analysis of the Escherichia coli PhoQ sensor kinase: differences with the Salmonella enterica serovar Typhimurium PhoQ protein and in the mechanism of Mg2+ and Ca2+ sensing. Regelmann, A.G., Lesley, J.A., Mott, C., Stokes, L., Waldburger, C.D. J. Bacteriol. (2002) [Pubmed]
  10. Salmonella typhimurium proliferates and establishes a persistent infection in the intestine of Caenorhabditis elegans. Aballay, A., Yorgey, P., Ausubel, F.M. Curr. Biol. (2000) [Pubmed]
  11. Co-regulation of Salmonella enterica genes required for virulence and resistance to antimicrobial peptides by SlyA and PhoP/PhoQ. Navarre, W.W., Halsey, T.A., Walthers, D., Frye, J., McClelland, M., Potter, J.L., Kenney, L.J., Gunn, J.S., Fang, F.C., Libby, S.J. Mol. Microbiol. (2005) [Pubmed]
  12. Molecular analysis of the Escherichia coli phoP-phoQ operon. Kasahara, M., Nakata, A., Shinagawa, H. J. Bacteriol. (1992) [Pubmed]
  13. Repression of Escherichia coli PhoP-PhoQ signaling by acetate reveals a regulatory role for acetyl coenzyme A. Lesley, J.A., Waldburger, C.D. J. Bacteriol. (2003) [Pubmed]
  14. Genome-wide analyses revealing a signaling network of the RcsC-YojN-RcsB phosphorelay system in Escherichia coli. Hagiwara, D., Sugiura, M., Oshima, T., Mori, H., Aiba, H., Yamashino, T., Mizuno, T. J. Bacteriol. (2003) [Pubmed]
  15. Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli. Minagawa, S., Ogasawara, H., Kato, A., Yamamoto, K., Eguchi, Y., Oshima, T., Mori, H., Ishihama, A., Utsumi, R. J. Bacteriol. (2003) [Pubmed]
 
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