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Gene Review

ECs4160  -  DNA-directed RNA polymerase subunit alpha

Escherichia coli O157:H7 str. Sakai

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Disease relevance of ECs4160


High impact information on ECs4160


Chemical compound and disease context of ECs4160


Biological context of ECs4160


Anatomical context of ECs4160


Associations of ECs4160 with chemical compounds

  • Finally, we used alanine scanning to identify determinants in the C-terminal domain of the RNA polymerase alpha subunit that are important for MelR-dependent activation of the melAB promoter [20].
  • The highest level of CAP-independent lac expression (13-fold the level of the wild-type lac promoter) correlated with changes in the -40 to -45 sequence and required an intact RNA polymerase alpha subunit for in vitro expression, as expected for an upstream DNA recognition element [21].
  • Site-directed mutagenesis of a negatively charged patch ((162)EEDE(165)) within the N-terminal domain of the RNAP alpha subunit that interacts with the positively charged AR2 of the cyclic AMP receptor protein suggested that Lys49 and Lys50 of FNR interact with this region of the alpha subunit of RNAP [22].

Analytical, diagnostic and therapeutic context of ECs4160


  1. Domain organization of RNA polymerase alpha subunit: C-terminal 85 amino acids constitute a domain capable of dimerization and DNA binding. Blatter, E.E., Ross, W., Tang, H., Gourse, R.L., Ebright, R.H. Cell (1994) [Pubmed]
  2. The COOH-terminal domain of the RNA polymerase alpha subunit in transcriptional enhancement and deactivation at the bacteriophage T4 late promoter. Tinker, R.L., Sanders, G.M., Severinov, K., Kassavetis, G.A., Geiduschek, E.P. J. Biol. Chem. (1995) [Pubmed]
  3. Molecular analysis of RNA polymerase alpha subunit gene from Streptomyces coelicolor A3(2). Cho, E.J., Bae, J.B., Kang, J.G., Roe, J.H. Nucleic Acids Res. (1996) [Pubmed]
  4. Substitution of the C-terminal domain of the Escherichia coli RNA polymerase alpha subunit by that from Bacillus subtilis makes the enzyme responsive to a Bacillus subtilis transcriptional activator. Mencía, M., Monsalve, M., Rojo, F., Salas, M. J. Mol. Biol. (1998) [Pubmed]
  5. Chlamydia trachomatis RNA polymerase alpha subunit: sequence and structural analysis. Gu, L., Wenman, W.M., Remacha, M., Meuser, R., Coffin, J., Kaul, R. J. Bacteriol. (1995) [Pubmed]
  6. Bipartite functional map of the E. coli RNA polymerase alpha subunit: involvement of the C-terminal region in transcription activation by cAMP-CRP. Igarashi, K., Ishihama, A. Cell (1991) [Pubmed]
  7. Transcription factor recognition surface on the RNA polymerase alpha subunit is involved in contact with the DNA enhancer element. Murakami, K., Fujita, N., Ishihama, A. EMBO J. (1996) [Pubmed]
  8. Positioning of two alpha subunit carboxy-terminal domains of RNA polymerase at promoters by two transcription factors. Murakami, K., Owens, J.T., Belyaeva, T.A., Meares, C.F., Busby, S.J., Ishihama, A. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  9. Determinants of RNA polymerase alpha subunit for interaction with beta, beta', and sigma subunits: hydroxyl-radical protein footprinting. Heyduk, T., Heyduk, E., Severinov, K., Tang, H., Ebright, R.H. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  10. Analysis of interactions between Activating Region 1 of Escherichia coli FNR protein and the C-terminal domain of the RNA polymerase alpha subunit: use of alanine scanning and suppression genetics. Lee, D.J., Wing, H.J., Savery, N.J., Busby, S.J. Mol. Microbiol. (2000) [Pubmed]
  11. Location of the C-terminal domain of the RNA polymerase alpha subunit in different open complexes at the Escherichia coli galactose operon regulatory region. Belyaeva, T.A., Bown, J.A., Fujita, N., Ishihama, A., Busby, S.J. Nucleic Acids Res. (1996) [Pubmed]
  12. Dimeric association of Escherichia coli RNA polymerase alpha subunits, studied by cleavage of single-cysteine alpha subunits conjugated to iron-(S)-1-[p-(bromoacetamido)benzyl]ethylenediaminetetraacetate. Miyake, R., Murakami, K., Owens, J.T., Greiner, D.P., Ozoline, O.N., Ishihama, A., Meares, C.F. Biochemistry (1998) [Pubmed]
  13. Dissecting DNA-protein and protein-protein interactions involved in bacterial transcriptional regulation by a sensitive protein array method combining a near-infrared fluorescence detection. Snapyan, M., Lecocq, M., Guével, L., Arnaud, M.C., Ghochikyan, A., Sakanyan, V. Proteomics (2003) [Pubmed]
  14. Transcription activation at class I FNR-dependent promoters: identification of the activating surface of FNR and the corresponding contact site in the C-terminal domain of the RNA polymerase alpha subunit. Williams, S.M., Savery, N.J., Busby, S.J., Wing, H.J. Nucleic Acids Res. (1997) [Pubmed]
  15. Novel protein--protein interaction between Escherichia coli SoxS and the DNA binding determinant of the RNA polymerase alpha subunit: SoxS functions as a co-sigma factor and redeploys RNA polymerase from UP-element-containing promoters to SoxS-dependent promoters during oxidative stress. Shah, I.M., Wolf, R.E. J. Mol. Biol. (2004) [Pubmed]
  16. Spacing requirements for interactions between the C-terminal domain of the alpha subunit of Escherichia coli RNA polymerase and the cAMP receptor protein. Lloyd, G.S., Busby, S.J., Savery, N.J. Biochem. J. (1998) [Pubmed]
  17. Similarity and divergence between the RNA polymerase alpha subunits from hyperthermophilic Thermotoga maritima and mesophilic Escherichia coli bacteria. Braun, F., Marhuenda, F.B., Morin, A., Guevel, L., Fleury, F., Takahashi, M., Sakanyan, V. Gene (2006) [Pubmed]
  18. Metal- and DNA-binding properties and mutational analysis of the transcription activating factor, B, of coliphage 186: a prokaryotic C4 zinc-finger protein. Pountney, D.L., Tiwari, R.P., Egan, J.B. Protein Sci. (1997) [Pubmed]
  19. Spinach plastid genes coding for initiation factor IF-1, ribosomal protein S11 and RNA polymerase alpha-subunit. Sijben-Müller, G., Hallick, R.B., Alt, J., Westhoff, P., Herrmann, R.G. Nucleic Acids Res. (1986) [Pubmed]
  20. Transcription activation at the Escherichia coli melAB promoter: interactions of MelR with the C-terminal domain of the RNA polymerase alpha subunit. Grainger, D.C., Belyaeva, T.A., Lee, D.J., Hyde, E.I., Busby, S.J. Mol. Microbiol. (2004) [Pubmed]
  21. The -45 region of the Escherichia coli lac promoter: CAP-dependent and CAP-independent transcription. Czarniecki, D., Noel, R.J., Reznikoff, W.S. J. Bacteriol. (1997) [Pubmed]
  22. Transcription activation by FNR: evidence for a functional activating region 2. Blake, T., Barnard, A., Busby, S.J., Green, J. J. Bacteriol. (2002) [Pubmed]
  23. Antigenic variability of bacterial RNA polymerases. Nikiforov, V.G., Lebedev, A.N., Kalyaeva, E.S. Mol. Gen. Genet. (1981) [Pubmed]
  24. Molecular cloning of the gene encoding RNA polymerase alpha subunit from deep-sea barophilic bacterium. Nakasone, K., Kato, C., Horikoshi, K. Biochim. Biophys. Acta (1996) [Pubmed]
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