Disease relevance of Pulse Radiolysis

• A pulse-radiolysis study of the manganese-containing superoxide dismutase from Bacillus stearothermophilus [1].
• Electron transfer process in cytochrome bd-type ubiquinol oxidase from Escherichia coli revealed by pulse radiolysis [2].
• Single-site mutants of the blue, single-copper protein, azurin, from Pseudomonas aeruginosa were reduced by CO2- radicals in pulse radiolysis experiments [3].
• A pulse-radiolysis study of cytochrome c3. Kinetics of the reduction of cytochrome c3 by methyl viologen radicals and the characterisation of the redox properties of cytochrome c3 from Desulfovibrio vulgaris (Hildenborough) [4].

High impact information on Pulse Radiolysis

• Internal electron transfer from the [(NH3)5RuII-] centre to the Fe(III) haem centre occurs with a rate constant k congruent to 53 s-1 (25 degrees C) (delta H = 3.5 kcal mol-1, delta S = -39 EU), as measured by pulse radiolysis [5].
• Equivalent results are obtained by pulse radiolysis using isopropanol radical as the reducing agent [6].
• We have studied the reaction kinetics of ten manganese porphyrins, differing in their meso substituents, with peroxynitrite (ONOO-) and carbonate radical anion (CO3.) using stopped-flow and pulse radiolysis, respectively [7].
• 1. The nitration yield was found to be dose rate-dependent, and the yield per radical produced by pulse radiolysis was identical to that obtained with authentic peroxynitrite [8].
• Purified metal-free (apoproteins) and various remetallated derivatives were analyzed by metal titrations monitored by UV-visible spectroscopy, histidine modification studies using diethylpyrocarbonate, and enzymatic activity measurements using pulse radiolysis [9].

Biological context of Pulse Radiolysis

• The kinetics of electron transfer within the molybdoflavoenzyme xanthine oxidase has been investigated using the technique of pulse radiolysis [10].
• The protective activities of four ginger-derived phenolic 1,3-diketones (1-4) and curcumin (5) against lipid peroxidation was studied by using different biologically relevant model systems and pulse radiolysis [11].
• To test thioredoxin resistance to oxidizing free radicals, we have studied the one-electron oxidation of wild-type thioredoxin and of two forms with the point mutations D30A and W35A, using azide radicals generated by gamma-ray or pulse radiolysis [12].
• Radiation effect on poly(vinylbenzyltrimethylammonium chloride) in aqueous solution: pulse radiolysis and steady-state study [13].

Associations of Pulse Radiolysis with chemical compounds

• The reaction of superoxide radical (O2-) with the heme protein indoleamine 2,3-dioxygenase has been investigated by the use of pulse radiolysis [14].
• The reduction of methemerythrin by e-aq and CO2- from pulse radiolysis studies [15].
• Electron transfer in milk xanthine oxidase as studied by pulse radiolysis [16].
• A direct demonstration of the catalytic action of monodehydroascorbate reductase by pulse radiolysis [17].
• The absorption spectra of the hydroxycyclohexadienyl radicals formed upon the addition of OH radicals to six substrates for phenol hydroxylase have been determined using pulse radiolysis [18].

Gene context of Pulse Radiolysis

• Moreover, with the use of pulse radiolysis, we show that human peroxiredoxin 5 reduces peroxynitrite with an unequalled high rate constant of (7+/-3)x10(7) M(-1)s(-1) [19].
• Mechanisms of free radical scavenging by the nutritional antioxidant beta-carotene have been investigated by pulse radiolysis [20].
• We have studied the time course of the absorption of bovine liver catalase after pulse radiolysis with oxygen saturation in the presence and absence of superoxide dismutase [21].
• Kinetic study by pulse radiolysis of the lactate dehydrogenase-catalyzed chain oxidation of nicotinamide adenine dinucleotide by HO2 and O2-RADICALS [22].
• Electron transfer process in milk xanthine dehydrogenase as studied by pulse radiolysis [23].

Analytical, diagnostic and therapeutic context of Pulse Radiolysis

• Time-resolved resonance Raman spectroscopy of cytochrome c reduced by pulse radiolysis [24].

References