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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

Endpoint Determination

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Disease relevance of Endpoint Determination


High impact information on Endpoint Determination

  • Many attempts have been made to design universal ligand-screening systems such that any GPCR can be screened using a common assay end-point [2].
  • With the three endpoint assays, each of which was applied 12 times at 2-hour intervals, maximum apoptotic responses varied from 22.5 to 72% in etoposide-treated cells and from 30 to 57% in cisplatin-treated cells [3].
  • To detect activated Stat5 in CML patient specimens, we developed an immunocytochemical assay that can be used as a molecular end-point assay to monitor inhibition of Bcr-Abl signaling [4].
  • Potential difference measurements could form a useful functional endpoint assay for future studies of either the CFTR gene or protein-based therapies in future trials in the pediatric age group [1].
  • Tumor samples were tested for sensitivity to Apomine, carboplatin, cisplatin, paclitaxel, and topotecan using an in vitro clonogenic [(3)H]thymidine end point assay [5].

Biological context of Endpoint Determination


Anatomical context of Endpoint Determination

  • Whereas the interleukin-8 induced locomotory changes were correctly represented by end point determination, relatively slight but significant modulation in lymphocyte behaviour by anti-integrin antibodies was revealed solely by analysis of the complete cell trajectory [7].

Associations of Endpoint Determination with chemical compounds


Gene context of Endpoint Determination

  • Lysyl oxidase activity in biological samples is traditionally and most reliably assessed by tritium release end-point assays using radiolabeled collagen or elastin substrates involving laborious vacuum distillation of the released tritiated water [13].
  • The advantages of this assay include the use of noninfectious antigens, the requirement for only small quantities of serum, the capability of screening large numbers of sera, the speed of execution, and the objectivity of end point determination [14].
  • There was close agreement of endpoint determinations of sera by both methods [15].
  • Accurate end-point determinations of the maximally oxygenated and deoxygenated myoglobin were made [16].
  • Monoclonal antibody 47-10/10, prepared using D80-100 virus and directed at the NS1 non-structural glycoprotein, had a significantly lower (100-fold) solid phase radioimmune assay endpoint titre for D80-141 antigen than for D80-100 antigen [17].

Analytical, diagnostic and therapeutic context of Endpoint Determination


  1. Potential difference measurements in the lower airway of children with and without cystic fibrosis. Davies, J.C., Davies, M., McShane, D., Smith, S., Chadwick, S., Jaffe, A., Farley, R., Collins, L., Bush, A., Scallon, M., Pepper, J., Geddes, D.M., Alton, E.W. Am. J. Respir. Crit. Care Med. (2005) [Pubmed]
  2. Chimaeric G alpha proteins: their potential use in drug discovery. Milligan, G., Rees, S. Trends Pharmacol. Sci. (1999) [Pubmed]
  3. Comparative analysis of different methodological approaches to the in vitro study of drug-induced apoptosis. Kravtsov, V.D., Daniel, T.O., Koury, M.J. Am. J. Pathol. (1999) [Pubmed]
  4. Inhibition of Bcr-Abl kinase activity by PD180970 blocks constitutive activation of Stat5 and growth of CML cells. Huang, M., Dorsey, J.F., Epling-Burnette, P.K., Nimmanapalli, R., Landowski, T.H., Mora, L.B., Niu, G., Sinibaldi, D., Bai, F., Kraker, A., Yu, H., Moscinski, L., Wei, S., Djeu, J., Dalton, W.S., Bhalla, K., Loughran, T.P., Wu, J., Jove, R. Oncogene (2002) [Pubmed]
  5. Phase I pharmacokinetic trial and correlative in vitro phase II tumor kinetic study of Apomine (SR-45023A), a novel oral biphosphonate anticancer drug. Alberts, D.S., Hallum, A.V., Stratton-Custis, M., Garcia, D.J., Gleason-Guzman, M., Salmon, S.E., Santabarbara, P., Niesor, E.J., Floret, S., Bentzen, C.L. Clin. Cancer Res. (2001) [Pubmed]
  6. A fully automated enzyme immunoassay for the measurement of cortisol in biological fluids. Bacarese-Hamilton, T., Cattini, R., Shandley, C., Howard, C., Palmer, R., McFarthing, K. European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies. (1992) [Pubmed]
  7. Lymphocyte locomotion in three-dimensional collagen gels. Comparison of three quantitative methods for analysing cell trajectories. Friedl, P., Noble, P.B., Zänker, K.S. J. Immunol. Methods (1993) [Pubmed]
  8. Quantitation of Candida albicans ergosterol content improves the correlation between in vitro antifungal susceptibility test results and in vivo outcome after fluconazole treatment in a murine model of invasive candidiasis. Arthington-Skaggs, B.A., Warnock, D.W., Morrison, C.J. Antimicrob. Agents Chemother. (2000) [Pubmed]
  9. Comparison of visual and spectrophotometric methods of broth microdilution MIC end point determination and evaluation of a sterol quantitation method for in vitro susceptibility testing of fluconazole and itraconazole against trailing and nontrailing Candida isolates. Arthington-Skaggs, B.A., Lee-Yang, W., Ciblak, M.A., Frade, J.P., Brandt, M.E., Hajjeh, R.A., Harrison, L.H., Sofair, A.N., Warnock, D.W. Antimicrob. Agents Chemother. (2002) [Pubmed]
  10. An integrated approach to lipid profiling: enzymatic determination of cholesterol and triglycerides with a centrifugal analyzer. Wentz, P.W., Cross, R.E., Savory, J. Clin. Chem. (1976) [Pubmed]
  11. Direct assays of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate with a centrifugal analyzer. Hansen, J.L., Freier, E.F. Clin. Chem. (1978) [Pubmed]
  12. Enzyme-coupled measurement of uric acid in serum with a centrifugal analyzer. White, R.M., Cross, R.E., Savory, J. Clin. Chem. (1977) [Pubmed]
  13. A fluorometric assay for detection of lysyl oxidase enzyme activity in biological samples. Palamakumbura, A.H., Trackman, P.C. Anal. Biochem. (2002) [Pubmed]
  14. Antigenic analysis of strains of Ebola virus: identification of two Ebola virus serotypes. Richman, D.D., Cleveland, P.H., McCormick, J.B., Johnson, K.M. J. Infect. Dis. (1983) [Pubmed]
  15. Detection of antibody to murine cytomegalovirus by enzyme-linked immunosorbent and indirect immunofluorescence assays. Classen, D.C., Morningstar, J.M., Shanley, J.D. J. Clin. Microbiol. (1987) [Pubmed]
  16. Cardiac performance as a function of intracellular oxygen tension in buffer-perfused hearts. Schenkman, K.A. Am. J. Physiol. Heart Circ. Physiol. (2001) [Pubmed]
  17. Variation in dengue type 2 viruses isolated in Bangkok during 1980. Walker, P.J., Henchal, E.A., Blok, J., Repik, P.M., Henchal, L.S., Burke, D.S., Robbins, S.J., Gorman, B.M. J. Gen. Virol. (1988) [Pubmed]
  18. Synthesis of a cortisol-biotin conjugate and evaluation as a tracer in an immunoassay for salivary cortisol measurement. Dressendörfer, R.A., Kirschbaum, C., Rohde, W., Stahl, F., Strasburger, C.J. J. Steroid Biochem. Mol. Biol. (1992) [Pubmed]
  19. 3,3',5,5' - Tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay. Bos, E.S., van der Doelen, A.A., van Rooy, N., Schuurs, A.H. Journal of immunoassay. (1981) [Pubmed]
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