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MeSH Review

Operator Regions (Genetics)

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Disease relevance of Operator Regions (Genetics)


High impact information on Operator Regions (Genetics)

  • Purified Mu repressor, in addition to its primary binding in the operator region, also binds less strongly to the Mu ends at the same sites as the Mu A protein [6].
  • Here we present the high-resolution crystal structure of the trp operator region that is most important in the recognition process [7].
  • We report here that the N-terminal region of Mu transposase contains two distinct DNA-binding domains, one which binds the two Mu DNA ends, and another which binds an internal operator region [8].
  • We propose that the interaction of met repressor with DNA occurs through either a pair of symmetry-related alpha-helices or a pair of beta-strands, and suggest a model for binding of several dimers to met operator regions [9].
  • An increased frequency (3-fold) of highly focused base substitutions was also observed at 2 sites in the lac operator region (at lacO +6, which is a transition "hotspot" in the spontaneous spectra of both wild type and uvrB- organisms and at the adjacent +5 site) [10].

Chemical compound and disease context of Operator Regions (Genetics)


Biological context of Operator Regions (Genetics)


Associations of Operator Regions (Genetics) with chemical compounds

  • Purified ExpR, an N-acyl homoserine lactone-responsive regulatory protein, binds to the promoter/operator region of the expI and expR genes [16].
  • Cyclic AMP promotes specific binding to a DNA fragment encoding the lac operator region; the K(d) for the protein-DNA binding is approximately 200 nM, which is 2-fold higher than the K(d) for CAP under identical conditions [17].
  • Three 20-bp inverted repeated DNA segments (subsites O1, O2, and O3) and the two divergent (PL and PR) promoters were mapped within the 153-bp operator region [5].
  • The MerR protein bound less tightly to its operator region (ca. 50- to 100-fold) in the presence of mercuric ion; this reduced affinity was largely accounted for by an increased rate of dissociation of the MerR protein from the DNA [18].
  • The distribution of doxorubicin-induced mutants among subclasses of mutation involving the i-d and lac operator regions differed significantly between repair-proficient and -deficient strains [19].

Gene context of Operator Regions (Genetics)

  • In contrast, the homologous promoter/operator regions of argI and argF did not appear to share any homologies with pyrB [20].
  • One hundred forty nucleotides upstream of the beta-lactamase start codon were determined for an inducible staphylococcal beta-lactamase and were identical to those of the constitutively expressed enterococcal gene, indicating that the changes resulting in constitutive expression are not due to changes in the promoter or operator region [21].
  • The Eagle-type mutant strains harbored no mutation in the mecI gene or in the operator region of mecA gene to which MecI repressor is supposed to bind [22].
  • Mutants of one group had a mutation in the operator region of kdgT; mutants of the other group had a mutation in kdgR, a regulatory gene controlling kdgT expression [23].
  • This also supports our earlier suggestion regarding the possible complexity of the internal operator region situated between argE and C [24].


  1. DNA sequences of the repressor gene and operator region of bacteriophage P2. Ljungquist, E., Kockum, K., Bertani, L.E. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  2. Purification and characterization of the diphtheria toxin repressor. Schmitt, M.P., Twiddy, E.M., Holmes, R.K. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  3. Analysis of diversity of mutations in the mecI gene and mecA promoter/operator region of methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. Kobayashi, N., Taniguchi, K., Urasawa, S. Antimicrob. Agents Chemother. (1998) [Pubmed]
  4. Studies of the operator region of the Staphylococcus aureus beta-lactamase operon. Clarke, S.R., Dyke, K.G. J. Antimicrob. Chemother. (2001) [Pubmed]
  5. A2 cro, the lysogenic cycle repressor, specifically binds to the genetic switch region of Lactobacillus casei bacteriophage A2. Ladero, V., García, P., Alonso, J.C., Suárez, J.E. Virology (1999) [Pubmed]
  6. Site-specific recognition of the bacteriophage Mu ends by the Mu A protein. Craigie, R., Mizuuchi, M., Mizuuchi, K. Cell (1984) [Pubmed]
  7. Determinants of repressor/operator recognition from the structure of the trp operator binding site. Shakked, Z., Guzikevich-Guerstein, G., Frolow, F., Rabinovich, D., Joachimiak, A., Sigler, P.B. Nature (1994) [Pubmed]
  8. Interaction of distinct domains in Mu transposase with Mu DNA ends and an internal transpositional enhancer. Leung, P.C., Teplow, D.B., Harshey, R.M. Nature (1989) [Pubmed]
  9. Three-dimensional crystal structures of Escherichia coli met repressor with and without corepressor. Rafferty, J.B., Somers, W.S., Saint-Girons, I., Phillips, S.E. Nature (1989) [Pubmed]
  10. DNA sequence specificity of doxorubicin-induced mutational damage in uvrB- Escherichia coli. Anderson, R.D., Veigl, M.L., Baxter, J., Sedwick, W.D. Cancer Res. (1991) [Pubmed]
  11. Expression plasmid vectors containing Escherichia coli tryptophan promoter transcriptional units lacking the attenuator. Tacon, W.C., Bonass, W.A., Jenkins, B., Emtage, J.S. Gene (1983) [Pubmed]
  12. Cyclic AMP-dependent constitutive expression of gal operon: use of repressor titration to isolate operator mutations. Irani, M., Orosz, L., Busby, S., Taniguchi, T., Adhya, S. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
  13. Model for regulation of Escherichia coli DNA repair functions. Gudas, L.J., Pardee, A.B. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
  14. In vitro transcription of the Escherichia coli K-12 argA, argE, and argCBH operons. Sens, D., Natter, W., James, E. J. Bacteriol. (1977) [Pubmed]
  15. An experimental and theoretical study of the inhibition of Escherichia coli lac operon gene expression by antigene oligonucleotides. Cheng, B., Fournier, R.L., Relue, P.A., Schisler, J. Biotechnol. Bioeng. (2001) [Pubmed]
  16. Integration of the quorum-sensing system in the regulatory networks controlling virulence factor synthesis in Erwinia chrysanthemi. Reverchon, S., Bouillant, M.L., Salmond, G., Nasser, W. Mol. Microbiol. (1998) [Pubmed]
  17. A functioning chimera of the cyclic nucleotide-binding domain from the bovine retinal rod ion channel and the DNA-binding domain from catabolite gene-activating protein. Scott, S.P., Weber, I.T., Harrison, R.W., Carey, J., Tanaka, J.C. Biochemistry (2001) [Pubmed]
  18. Homologous metalloregulatory proteins from both gram-positive and gram-negative bacteria control transcription of mercury resistance operons. Helmann, J.D., Wang, Y., Mahler, I., Walsh, C.T. J. Bacteriol. (1989) [Pubmed]
  19. Excision repair reduces doxorubicin-induced genotoxicity. Anderson, R.D., Veigl, M.L., Baxter, J., Sedwick, W.D. Mutat. Res. (1993) [Pubmed]
  20. The DNA sequence of argI from Escherichia coli K12. Bencini, D.A., Houghton, J.E., Hoover, T.A., Foltermann, K.F., Wild, J.R., O'Donovan, G.A. Nucleic Acids Res. (1983) [Pubmed]
  21. Nucleotide sequence of the beta-lactamase gene from Enterococcus faecalis HH22 and its similarity to staphylococcal beta-lactamase genes. Zscheck, K.K., Murray, B.E. Antimicrob. Agents Chemother. (1991) [Pubmed]
  22. Eagle-type methicillin resistance: new phenotype of high methicillin resistance under mec regulator gene control. Kondo, N., Kuwahara-Arai, K., Kuroda-Murakami, H., Tateda-Suzuki, E., Hiramatsu, K. Antimicrob. Agents Chemother. (2001) [Pubmed]
  23. 2-keto-3-deoxygluconate transport system in Erwinia chrysanthemi. Condemine, G., Robert-Baudouy, J. J. Bacteriol. (1987) [Pubmed]
  24. Parameters of gene expression in the bipolar argECBH operon of E. coli K12. The question of translational control. Cunin, R., Boyen, A., Pouwels, P., Glansdorff, N., Crabeel, M. Mol. Gen. Genet. (1975) [Pubmed]
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