Disease relevance of 2-deoxy-D-glucose

• The glycosylation inhibitors 2-deoxy-D-glucose (2-dGlc) and, to a lesser extent, beta-hydroxynorvaline blocked the formation of syncytia in HIV (LAV/HTLV-III)-infected cells [1].
• Administration of 2-dGlc (1 mg/100 g body weight) produced a decrease in the tissue total glycosaminoglycans level [2].

Psychiatry related information on 2-deoxy-D-glucose

• For example, estrous cycles are inhibited by food deprivation or treatment with 2-deoxy-D-glucose (2DG), a drug that inhibits cellular glucose utilization [3].

High impact information on 2-deoxy-D-glucose

• RESULTS: CGRP produced a dose related inhibition of pancreatic secretion evoked by 2DG [4].
• CONCLUSIONS: Our data indicates that CGRP inhibits pancreatic enzyme secretion evoked by 2DG or CCK-8 via vagal pathways [4].
• The pattern of 2DG-induced gene expression indicates that the ventrolateral medulla contains the vast majority of catecholamine neurons responsive to glucoprivation [5].
• The inhibition of 2-dGlc uptake is consistent with a decrease in the coupling between endofacial hexokinase activity and the sugar transporter [6].
• Dexamethasone decreases 2-D-deoxyglucose (2-dGlc) uptake and accumulation into rat peritoneal macrophages in vitro in a concentration- and time-dependent manner (Ki for 1 microM-dexamethasone after a 2 h exposure = 0.71 +/- 0.21 microM; Ki for 0.1 microM-dexamethasone after exposure for 4 h = 0.10 +/- 0.06 microM) [6].

Biological context of 2-deoxy-D-glucose

• Subsequently, both groups were treated with doses of 2DG known to inhibit the estrous cycle (1750 mg/kg every 6 h on days 1 and 2 of the cycle) [3].
• This study was designed to examine the role of the AP in 2DG-induced anestrus [3].
• Bilateral cell counts from representative levels of the LHA, DMN, and PHA showed that in each structure, a greater proportion of ORX-A neurons were immunostained for Fos in response to systemic than following i.c.v. treatment with 2DG [7].
• In contrast, in the presence of 2DG or 2,5-AM, E reduced food intake only by 22% and 24%, respectively (not significant) [8].
• Intracellular phosphorylation of 2DG abstracts phosphorus from its normal pathways [9].

Anatomical context of 2-deoxy-D-glucose

• 1. Suspensions of rat thymocytes accumulate free 2-deoxy-D-glucose (2-dGlc) within the cytosol to a concentration approx. 25-fold above the external concentration [10].
• The mCSF-dependent increase in 2-dGlc uptake by macrophages was enhanced by preincubation of the cells in mCSF-free solution [11].
• Thus, 2DG induces ERalpha expression in specific brain nuclei and expression of ERalpha in catecholaminergic neurons of the brainstem indicates a role for estrogen in activating those neurons projecting to the hypothalamic paraventricular nucleus to suppress LH secretion during glucoprivation [12].

Associations of 2-deoxy-D-glucose with other chemical compounds

• 4. Coupling of 2-dGlc transport to hexokinase activity was determined by observing the effects of various concentrations of unlabelled cytosolic 2-dGlc on influx of labelled 2-dGlc into the hexose phosphate pool [10].
• The inhibition of protein synthesis by cycloheximide did not prevent the relative stimulation of 2-dGlc and choline utilization by serum and pH treatment [13].
• 5. The high-affinity inhibitor of hexokinase, mannoheptulose, inhibited uptake of 2-dGlc in both control and phorbol-treated cells [10].
• In the muscle loaded with 2-dGlc "in vitro" (this sugar is accumulated as hexosephosphate) glycogen synthase I levels are changed by a mechanism which is additive to those of hormones such as insulinor epinephrine [14].

Analytical, diagnostic and therapeutic context of 2-deoxy-D-glucose

• Therefore, rats implanted with fourth ventricular cannulas were tested for feeding and glycemic responses to centrally administered 5-thioglucose (5TG, 120 micrograms/5 microliter) and subcutaneously administered 2-deoxy-D-glucose (2DG, 250 mg/kg, IP) [15].
• Oxygen consumption per unit work remained constant during 2DG perfusion [9].

References