High impact information on SLC22A7

• The human organic anion transporter 2 (hOAT2, SLC22A7) mediates the sodium-independent uptake of numerous drugs, including cephalosporins, salicylates, dicarboxylates, and prostaglandins, and is mainly expressed in hepatocytes [1].
• Because HNF-4 alpha is a known target for bile acid-mediated repression of gene transcription, we studied whether chenodeoxycholic acid (CDCA) suppresses hOAT2 gene expression by inhibiting HNF-4 alpha-mediated transactivation [1].
• Only ranitidine was efficiently transported by hOAT2 (Km, 396 microM). rOct1 accepts all of the H2 receptor antagonists with a similar activity, whereas the transport activities of ranitidine and famotidine (Km, 61/56 microM) by r/hOCT2 were markedly lower than that of cimetidine (Km, 69/73 microM) [2].
• The K(i) value of probenecid for hOAT2 was 766 microM, whereas those of probenecid, KW-3902, and betamipron for hOAT4 were 54.9, 20.7, and 502 microM, respectively [3].
• Probenecid, but not KW-3902, betamipron, and cilastatin, significantly inhibited hOAT2-mediated PGF(2alpha) uptake [3].

Biological context of SLC22A7

• Assignment of liver-specific organic anion transporter (SLC22A7) to human chromosome 6 bands p21.2-->p21.1 using radiation hybrids [4].
• Transport mechanism and substrate specificity of human organic anion transporter 2 (hOat2 [SLC22A7]) [5].
• The amino acid sequence of NLT shows 42% identity to that of the renal multispecific organic anion transporter, OAT1 [6].

Anatomical context of SLC22A7

• When expressed in Xenopus laevis oocytes, NLT mediated uptake of organic anions, such as salicylate, acetylsalicylate, PGE2, dicarboxylates and p-aminohippurate [6].
• The ChE activity noticed in some parts of the ependyma and in some NLT cells suggest their probable differential cholinergic control [7].

Associations of SLC22A7 with chemical compounds

• Cells expressing hOAT2 showed uptake of p-aminohippurate, methotrexate, cAMP, and alpha-ketoglutarate [8].
• Bile acid transporters, i.e., bile salt export pump and Na(+)-taurocholate cotransporting polypeptide, and the sinusoidal transporter, OAT2, were down-regulated by all the tested chemicals [9].
• In addition, oxytetracycline, minocycline and doxycycline inhibited the organic anion uptake by hOAT1, whereas oxytetracycline, minocycline but not doxycycline inhibited the organic anion uptake by hOAT2 [10].
• The aim of this study was to examine whether mOAT2/3, as well as hOAT2/3, transports the diuretic bumetanide using a Xenopus laevis oocyte expression system [11].
• Varying degrees of ascorbic acid activity are noticed inside the ventricle, among the tanycytes and in the neurons of the NLT [7].

Other interactions of SLC22A7

• The effects of OAT inhibitors on OAT1, OAT2, and OAT3 exhibited some but not so remarkable interspecies differences between humans and rats [12].
• We further isolated homologs of OAT series such as liver-specific OAT2 and kidney-, liver- and brain-expressing OAT3 [13].
• The levels of Mrp2, Oat1 and Oat2 on day 1 after the treatment showed no significant change [14].
• Short interfering RNAs inhibiting endogenous HNF-4 alpha expression markedly reduced endogenous expression of hOAT2 in Huh7 cells [1].

References